Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	718,690	0	T	G	45.7%	44.1 / 19.8	35	T591P (ACC→CCC)	speF	ornithine decarboxylase isozyme, inducible
Reads supporting (aligned to +/- strand):  ref base T (5/14);  new base G (16/0);  total (21/14)
Fisher's exact test for biased strand distribution p-value = 9.65e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCG  >  NC_000913/718540‑718827                                                                                                                                                       |                                                                                                                                          ccACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGt                                                                                                                                           <  2:558695/151‑1 (MQ=255)       cACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATGCATTTTCTTACACAACTGACGCAGGGGATaaac                                                                                                                                     >  2:347956/1‑149 (MQ=255)       cACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAAcc                                                                                                                                     <  1:284814/151‑1 (MQ=255)              tAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTa                                                                                                                               <  2:310624/150‑1 (MQ=255)               aaaTAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTTCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAg                                                                                                                              <  1:668113/150‑1 (MQ=255)               aaaTAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATAATGAATTTTCTGACACAAATGACGCAGGGGATaaac                                                                                                                                     >  2:100677/1‑141 (MQ=255)                       gTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACCTGCATTTTCTGTCACAACCGACGCAGGGGTTAAccagaggagag                                                                                                                            >  1:191403/1‑135 (MQ=255)                       gTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCAGACAAAACAAGCGTTTTCTGACACAACTAACGCCGGGGATAACCCGCGGAGCGCCCtt                                                                                                                       >  1:124494/1‑149 (MQ=255)                        tAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAATCATGCATTTCCTGTCACAAACGACGCAGGGGATAAACGGCGCAGCGCTCtt                                                                                                                       >  2:312710/1‑148 (MQ=255)                        tAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAATGCATTTCCTTTCACACCCGACGCAGGGGGTAACCGGCGTAGCGCTCtt                                                                                                                       >  1:162733/1‑148 (MQ=255)                         aGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCAAACAAAAAATTCATTTCCTTACACAAATGAAGCAGGGGATAAACCGCGTAACGCCCtt                                                                                                                       >  2:676142/1‑147 (MQ=255)                              gCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGtt                                                                                                              <  2:300170/151‑1 (MQ=255)                                ttcttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAAAAAGCTTTTTCTGGAACAAATGAAGCAGGGGATAAACCGGGTGGGGCTCTTTATGCTGttt                                                                                                             >  2:702030/1‑150 (MQ=255)                                     gCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAAGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGAt                                                                                                       <  2:637753/151‑1 (MQ=255)                                      cGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATg                                                                                                      <  2:550422/151‑1 (MQ=255)                                       ggATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTCCTTACCAAACAAAAGCCGGGGATAACCAGCGGAGCGCTCTTCATGCTGTTTGt                                                                                                           >  2:425289/1‑145 (MQ=255)                                        gATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGa                                                                                                    <  2:399916/151‑1 (MQ=255)                                              tGCTGACGCGTGGGACGTGCTCCTTCCGGCCCCTCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATAAAAATCAAACATTTTCTTCCACAACTGAACCCGGGGATAAACGCCGAAAAGCTTCTCATCGTGTTTTTAGATGGGAg                                                                                                  >  2:366129/1‑147 (MQ=255)                                               gCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATAAAAAAAAATGATTTTCTTGCACAAATAAAGCCGGGGAGAACCCGCGTCGCGCCCTTCTTTTTGTTTGTTGATGGAAgg                                                                                                 >  1:227271/1‑147 (MQ=255)                                                 tGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAAATCCTTTTCTGTCACAAATGACGCAGGGGGTAAACCGCGGAGCGCCCTTTCTGCTGTTTTTAGATGGAAgg                                                                                                 >  2:42107/1‑145 (MQ=255)                                                  gACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCAc                                                                                           <  2:465804/150‑1 (MQ=255)                                                    cgcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCAATCAAAACAAGCATTTTCTTTCACAAATTAAGCAGGGGATAACCCGGGGGGCATTCTTCATTTTGTTTTTAgggtggaggaa                                                                                               >  2:464992/1‑134 (MQ=255)                                                     gcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCt                                                                                       <  2:334819/151‑1 (MQ=255)                                                         gggAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTa                                                                                   <  1:765667/151‑1 (MQ=255)                                                            aaGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATACCTTTTCTTACCCAACCTACGCAGGGGGTAAACCGCGGAGCGCTCTTCCTGCTGTTTGTTGGTGGAAAGGAGCACctctt                                                                                      >  1:468593/1‑144 (MQ=255)                                                                              aTCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATAATGCATTTTCTTACACAAATGACGCAGGGGATAACCCGCGTAGCGCTCTTCATGCTGTTTTTTGGTGGGAGGGAGCACTTTTTCTAAAGGCGCGGCGGGCGCaa                                                                 >  2:552077/1‑148 (MQ=255)                                                                                  ctTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAg                                                             <  1:572473/148‑1 (MQ=255)                                                                                                 tCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACca                                            <  1:188723/150‑1 (MQ=255)                                                                                                  cACGTTGTGGCGGGCATACAAAACAATCATTTCCTTACCCAACTGACGCCGGGGGTAACACGCGTAGGGCCCTTCTTTCTGTTTGTTGTTGGAAGGTAGAACCTCTGCTCATTGCGGGTCGGGCCCCAGCAGGTTTTTTTAGCGTAcc                                             >  1:650371/1‑148 (MQ=255)                                                                                                           gCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGcaaca                                 <  2:674837/151‑1 (MQ=255)                                                                                                             gggCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGcaaca                                 >  1:119442/1‑149 (MQ=255)                                                                                                                  tACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCt                          >  1:393636/1‑151 (MQ=255)                                                                                                                            gCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGc                >  1:88487/1‑151 (MQ=255)                                                                                                                             cATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGcc               >  2:613149/1‑151 (MQ=255)                                                                                                                                     gACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATAtctt        <  1:334330/150‑1 (MQ=255)                                                                                                                                          aaCTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCg  >  2:474501/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                          CCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCG  >  NC_000913/718540‑718827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑