Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,178,172	T→G	F144V (TTC→GTC)	nusG →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,178,172	0	T	G	100.0%	242.4 / NA	62	F144V (TTC→GTC)	nusG	transcription termination factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/31);  total (31/31)

GTACTTCCGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG  >  NC_000913/4178026‑4178319                                                                                                                                                   |                                                                                                                                                    gTACTTCCGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCaa                                                                                                                                                 <  1:197503/151‑1 (MQ=255)   aCTTCCGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAAc                                                                                                                                                <  2:769812/150‑1 (MQ=255)   aCTTCCGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACg                                                                                                                                               >  2:146273/1‑151 (MQ=255)    cTTCCGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACgg                                                                                                                                              >  1:221943/1‑151 (MQ=255)     ttCCGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgt                                                                                                                                             >  2:710261/1‑151 (MQ=255)      tCCGATCGTCCCGCGCAAATCAGCGATAAAGAAGTCGATGCGATTAAGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgt                                                                                                                                             <  2:608989/150‑1 (MQ=255)       ccGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtg                                                                                                                                            <  1:745476/150‑1 (MQ=255)            cGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGttgttg                                                                                                                                      >  2:663026/1‑151 (MQ=255)            cGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGttgttg                                                                                                                                      >  2:809798/1‑151 (MQ=255)             gTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTga                                                                                                                                     <  2:673844/151‑1 (MQ=255)             gTCCGGCGCCAATCGGCGATAAAGAAGTCGATGCGATTAGGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTga                                                                                                                                     <  1:355428/151‑1 (MQ=255)              tCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTga                                                                                                                                     >  2:722948/1‑150 (MQ=255)                 tGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaaga                                                                                                                                  >  2:532971/1‑150 (MQ=255)                  gcgcCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaagaa                                                                                                                                 <  2:489564/150‑1 (MQ=255)                  gcgcCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaagaa                                                                                                                                 <  1:408045/150‑1 (MQ=255)                  gcgcCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaagaa                                                                                                                                 <  1:1760/150‑1 (MQ=255)                  gcgcCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaaga                                                                                                                                  <  2:190779/149‑1 (MQ=255)                     ccAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGt                                                                                                                               <  2:103618/149‑1 (MQ=255)                     ccAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTgg                                                                                                                             <  2:734516/151‑1 (MQ=255)                     ccAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTg                                                                                                                              <  1:389000/150‑1 (MQ=255)                       aaTCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGGGGAt                                                                                                                           >  1:126311/1‑151 (MQ=255)                         tCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGAtt                                                                                                                          <  1:255663/150‑1 (MQ=255)                             cGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACgag                                                                                                                     <  2:547346/151‑1 (MQ=255)                               aTAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaa                                                                                                                   <  2:48088/151‑1 (MQ=255)                                     aagTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCg                                                                                                             <  1:246843/151‑1 (MQ=255)                                         cGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTg                                                                                                         >  2:56569/1‑151 (MQ=255)                                            tGCGATTATGAACCGCCTGCAGAAGGTTGGTGATAAGGCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTAGTCTGaa                                                                                                       >  1:726694/1‑150 (MQ=255)                                             gCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGaa                                                                                                       >  2:59954/1‑149 (MQ=255)                                              cGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAgt                                                                                                    >  2:584154/1‑151 (MQ=255)                                                   aTGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTg                                                                                               >  1:392319/1‑151 (MQ=255)                                                     gAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGtt                                                                                             >  1:691303/1‑151 (MQ=255)                                                      aaCCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTc                                                                                                 <  2:117054/146‑1 (MQ=255)                                                              gcagcaGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCt                                                                                      <  1:600062/149‑1 (MQ=255)                                                               cagcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCg                                                                                   >  1:540602/1‑151 (MQ=255)                                                                   agGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCg                                                                               >  1:132243/1‑151 (MQ=255)                                                                                   gcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTagag                                                               <  2:799073/151‑1 (MQ=255)                                                                                   gcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTag                                                                 >  2:445260/1‑149 (MQ=255)                                                                                    cgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGc                                                              <  1:84852/151‑1 (MQ=255)                                                                                         gAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGAc                                                         >  2:490331/1‑151 (MQ=255)                                                                                          aaaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACt                                                        >  1:105882/1‑151 (MQ=255)                                                                                           aaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACtt                                                       >  2:649785/1‑151 (MQ=255)                                                                                            aaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTc                                                      <  2:536282/151‑1 (MQ=255)                                                                                            aaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTc                                                      <  2:88282/151‑1 (MQ=255)                                                                                               gCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGc                                                   <  2:44347/151‑1 (MQ=255)                                                                                               gCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGc                                                   <  2:251306/151‑1 (MQ=255)                                                                                                    ttGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGt                                              >  2:141399/1‑151 (MQ=255)                                                                                                          cGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGaaa                                         >  1:655655/1‑150 (MQ=255)                                                                                                              tGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGcc                                    >  2:190456/1‑151 (MQ=255)                                                                                                                  aTGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAAc                                <  2:495748/151‑1 (MQ=255)                                                                                                                     gTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCa                             <  1:677600/151‑1 (MQ=255)                                                                                                                       ccGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAg                            <  2:733079/150‑1 (MQ=255)                                                                                                                           gtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATc                       >  1:287781/1‑151 (MQ=255)                                                                                                                           gtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATc                       >  1:115592/1‑151 (MQ=255)                                                                                                                            ttAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGAGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATc                       >  1:53358/1‑150 (MQ=255)                                                                                                                              aaTGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaa                     >  1:763370/1‑150 (MQ=255)                                                                                                                              aaTGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaa                     >  1:548463/1‑150 (MQ=255)                                                                                                                                tgatgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaaaa                   >  1:675551/1‑150 (MQ=255)                                                                                                                                       ccGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggcg           >  2:647964/1‑151 (MQ=255)                                                                                                                                       ccGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggcg           >  1:448939/1‑151 (MQ=255)                                                                                                                                            cGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa      <  2:223263/151‑1 (MQ=255)                                                                                                                                            cGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa      <  1:450057/151‑1 (MQ=255)                                                                                                                                                gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCg  <  1:62309/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTACTTCCGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG  >  NC_000913/4178026‑4178319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑