Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,930,993	T→G	V38G (GTG→GGG)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,930,993	0	T	G	100.0%	221.3 / NA	57	V38G (GTG→GGG)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/29);  total (28/29)

CAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTG  >  NC_000913/1930866‑1931139                                                                                                                                |                                                                                                                                                   cAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCg                                                                                                                             <  1:627790/151‑1 (MQ=255) cAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCg                                                                                                                             >  1:544935/1‑151 (MQ=255) cAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCg                                                                                                                             >  2:700070/1‑151 (MQ=255) cAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCg                                                                                                                             >  1:771769/1‑151 (MQ=255) cAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCg                                                                                                                             >  2:578482/1‑151 (MQ=255)  aGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCg                                                                                                                             >  2:438095/1‑150 (MQ=255)       atatTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCAc                                                                                                                       >  2:509289/1‑151 (MQ=255)          ttCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCAcc                                                                                                                      >  1:35928/1‑149 (MQ=255)               tATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCcat                                                                                                                <  2:268789/150‑1 (MQ=255)                  gACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCcatgcat                                                                                                            <  1:700035/151‑1 (MQ=255)                    cGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGt                                                                                                          >  2:762382/1‑151 (MQ=255)                     gTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTc                                                                                                         <  1:261481/151‑1 (MQ=255)                       tattaGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgc                                                                                                       >  1:729303/1‑151 (MQ=255)                          taGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCgcgc                                                                                                     >  2:194514/1‑150 (MQ=255)                           aGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCAt                                                                                                   <  1:24155/151‑1 (MQ=255)                           aGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCAt                                                                                                   >  1:507372/1‑151 (MQ=255)                             gCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCg                                                                                                 <  1:684001/151‑1 (MQ=255)                               aCTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCt                                                                                               <  1:502786/151‑1 (MQ=255)                                   cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTccc                                                                                            >  2:472336/1‑150 (MQ=255)                                    gctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCAt                                                                                          <  2:729126/151‑1 (MQ=255)                                         gTCCGCCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGAGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCCTCGCTCCCATGTCa                                                                                      >  1:354518/1‑150 (MQ=255)                                            cGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTaa                                                                                  <  2:264161/151‑1 (MQ=255)                                              gcagcaACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAat                                                                                 >  1:144029/1‑150 (MQ=255)                                              gcagcaACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAat                                                                                 >  1:363522/1‑150 (MQ=255)                                                agcaACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATg                                                                              <  2:476891/151‑1 (MQ=255)                                                agcaACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATg                                                                              <  2:580295/151‑1 (MQ=255)                                                  caACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCt                                                                            <  2:241336/151‑1 (MQ=255)                                                   aaCTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCtt                                                                           <  1:704101/151‑1 (MQ=255)                                                       cgcgTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGAc                                                                                                                           >  2:301759/1‑99 (MQ=255)                                                       cgcgTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGAc                                                                                                                           <  1:301736/99‑1 (MQ=255)                                                       cgcgTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGa                                                                         <  1:22465/149‑1 (MQ=255)                                                          gTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTg                                                                    >  2:505231/1‑151 (MQ=255)                                                          gTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGGGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTg                                                                    >  1:482479/1‑151 (MQ=255)                                                            gATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGa                                                                   <  1:472303/150‑1 (MQ=255)                                                            gATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGa                                                                   <  2:209785/150‑1 (MQ=255)                                                            gATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAt                                                                  >  1:649161/1‑151 (MQ=255)                                                                 tattaGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCAtt                                                             >  1:617585/1‑151 (MQ=255)                                                                  attaGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCAtt                                                             >  2:329342/1‑150 (MQ=255)                                                                        cTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCgt                                                      >  2:552226/1‑151 (MQ=255)                                                                                 aCTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGa                                              >  1:92192/1‑150 (MQ=255)                                                                                    gggTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACt                                           <  1:495812/150‑1 (MQ=255)                                                                                    gggTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACt                                           <  2:576393/150‑1 (MQ=255)                                                                                       tAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGa                                        >  2:152327/1‑150 (MQ=255)                                                                                         aagaagTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGaaaa                                     <  1:358316/151‑1 (MQ=255)                                                                                                 gCAATCGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACAtt                             >  2:580249/1‑151 (MQ=255)                                                                                                   aaTCGAGTGTCAGCTTCTCGGCGTAGAGGGGATTGCCGCCGATCGCTATGCCGACGCACCAGCCATGCATGTAGCGCATCGCTCCCATGTCATTACTATGCTTGATGGTGATGCTTTACGCCGTGTGCTTGAACTGGAAAAACCACATtat                           >  1:358041/1‑151 (MQ=255)                                                                                                      cGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATc                        <  2:333376/151‑1 (MQ=255)                                                                                                      cGAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATc                        >  1:569684/1‑151 (MQ=255)                                                                                                       gAGTGTCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCg                       <  1:469169/151‑1 (MQ=255)                                                                                                           gtCAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGcc                   >  1:51554/1‑151 (MQ=255)                                                                                                             cAGCGTCTCGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCg                  <  2:35950/150‑1 (MQ=255)                                                                                                                     cGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATc            <  2:617619/148‑1 (MQ=255)                                                                                                                     cGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCg           <  1:201798/149‑1 (MQ=255)                                                                                                                     cGGCGTAGAGGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCATGATAGTGATGCATTACGCAGTGTGGTTGAACTGGAAAAACCACACTATATCGTGCCGGAGATCGa          <  1:137467/150‑1 (MQ=255)                                                                                                                           agagGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTAtt   <  1:67458/151‑1 (MQ=255)                                                                                                                            gagGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTg  <  1:62587/151‑1 (MQ=255)                                                                                                                            gagGGGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTg  <  2:703038/151‑1 (MQ=255)                                                                                                                                |                                                                                                                                                   CAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTG  >  NC_000913/1930866‑1931139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑