Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,668,878	IS2 (+) +5 bp	coding (307‑311/1398 nt)	yhjA ←	putative cytochrome C peroxidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2996361 =	NA (NA)	53 (1.220)	48/294	0.1	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	3668878 =	0 (0.000)					coding (311/1398 nt)	yhjA	putative cytochrome C peroxidase
*	?	NC_000913	3186096 =	NA (NA)	43 (0.970)	38/298	0.3	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 3668882	0 (0.000)					coding (307/1398 nt)	yhjA	putative cytochrome C peroxidase

TTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3186239‑3186096 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCCTGGATTG  <  NC_000913/3668882‑3668735                                                                                                                                                                                                                                                                                                      TTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTG                                                                                                                                               >  2:452485/1‑151  TTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTG                                                                                                                                                   <  2:510260/146‑1   TTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGA                                                                                                                                              <  2:696771/150‑1    TCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGAT                                                                                                                                             <  2:618530/150‑1    TCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGAT                                                                                                                                             >  2:62957/1‑150     CCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATA                                                                                                                                            <  2:148833/150‑1        GTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAA                                                                                                                                          <  1:453585/149‑1         TGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCC                                                                                                                                       >  2:196788/1‑151          GTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCG                                                                                                                                      >  1:88587/1‑151            GGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTT                                                                                                                                    <  2:635467/151‑1              TACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTC                                                                                                                                  >  1:696762/1‑151               ACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCG                                                                                                                                 >  2:345167/1‑151                        GCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGA                                                                                                                         <  2:663381/150‑1                                 CGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAG                                                                                                               <  1:435904/151‑1                                    CCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATT                                                                                                            >  2:163707/1‑151                                    CCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATT                                                                                                            >  1:319284/1‑151                                        AAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGA                                                                                                          <  2:166155/149‑1                                         AGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATG                                                                                                       <  2:376503/151‑1                                          GACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGG                                                                                                      <  2:254739/151‑1                                                AATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATG                                                                                                <  1:595101/151‑1                                                        GTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTAT                                                                                          <  2:443176/149‑1                                                         TTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGA                                                                                        >  2:411408/1‑150                                                           CTCCAATGACTAGTCTAAAAACTAGTATTAAGACTCTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAAC                                                                                     >  1:347938/1‑151                                                                    CTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACC                                                                            >  1:38868/1‑151                                                                    CTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACC                                                                            >  1:80232/1‑151                                                                        TCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACG                                                                        >  1:180447/1‑151                                                                        TCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACG                                                                        >  2:312232/1‑151                                                                            AAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTT                                                                    >  2:58015/1‑151                                                                              AACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTAT                                                                  >  2:554410/1‑151                                                                                    TATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCG                                                            >  2:402411/1‑151                                                                                         AGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACA                                                       <  2:527626/151‑1                                                                                         AGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACA                                                       <  2:549535/151‑1                                                                                           ACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACT                                                     >  2:660644/1‑151                                                                                            CTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTG                                                    >  2:639202/1‑151                                                                                                  CTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGG                                              >  1:187059/1‑151                                                                                                   TTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGG                                              >  1:178185/1‑150                                                                                                     ATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTA                                            >  2:19278/1‑150                                                                                                     ATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTATCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTA                                            >  2:281363/1‑150                                                                                                        TAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGCAAGG                                         >  1:581439/1‑150                                                                                                                          TGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGC                      >  2:212289/1‑151                                                                                                                                   AGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACT             <  1:660596/151‑1                                                                                                                                       GGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCC         <  2:455301/151‑1                                                                                                                                                ACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCCTGGATTG  <  2:652091/149‑1                                                                                                                                                                                                                                                                                                      TTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3186239‑3186096 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCCTGGATTG  <  NC_000913/3668882‑3668735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.