| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,412,906 | 0 | T | A | 45.8% | 26.2 / 10.3 | 24 | Y187F (TAT→TTT) | acrS | acrAB operon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (9/4); new base A (0/11); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAG > NC_000913/3412757‑3413050 | aaCTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTtat > 2:335587/1‑150 (MQ=255) gACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAAccc < 2:109938/150‑1 (MQ=255) aCGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGc < 2:455011/151‑1 (MQ=255) gAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTATTTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCa < 2:72969/151‑1 (MQ=255) ggAAAGAATGTAAATTTAATTCACACTTAATTCTTTTGTTTGATTAATTAATTTTT‑TTATTTTTTCATCTAGCATAAACATTCTTAATTCTTAATCACCCAGGGGGGGGGTTTTTTTAAAAAAATCATAACCCGCCATATTCATTAACCAg < 1:502327/151‑1 (MQ=255) cATGACACTTAATTCATTCGTTTGATGAATTAATTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTg > 2:452447/1‑151 (MQ=255) atcacatTTATTTCATTCTTTTAAAGAATTATTTTCT‑TTTTTTTTTCATCGGAAATAAACATTTTTAATCCGTTATCGACCAGAGGGGGGTTTTTTTTAAAAAAATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCt < 1:694137/146‑1 (MQ=255) aaTTCTTTCTTTTGATAAATTAATTTCT‑TTTTTTTTTCATCTGGCATAAACATTCTTAATACTTTATCGACCAGGGCGGGGGTTTTTTTAAAAAAATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCa < 2:659382/150‑1 (MQ=255) ttaattaaataattTCCTTTATGTTTTAATCTGGCATAAACTTTTTTAATCCTTTTTCGCCCGGGGGGGGGGTTTTTTTAAAAATACCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaataat < 2:199783/139‑1 (MQ=255) ttGATGAATTAATTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAatc > 2:553460/1‑151 (MQ=255) atgaATTAATTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAatcatc > 1:75481/1‑151 (MQ=255) atgaATTAATTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAatcatc > 2:395848/1‑151 (MQ=255) atttatttTCT‑TTTTTTTTTCTTTTGGAAAAAACATTTTTAATTCGTTAACGCCCAGGGGGGGGTTTTGTTTAAAAAGATCAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAAtcatca < 1:425104/147‑1 (MQ=255) taattTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCacaac > 1:409560/1‑150 (MQ=255) tttGTTTTCATTTGCCATAAACATTATAAATTCTCTAGCACCCAGAGCGGGGTTTTTTTAAAAAAGTTAACAGCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATc < 1:265783/148‑1 (MQ=255) tGTTTTCATCTGTAAAAAACATTCTAAATCCTTTATCGCCCAGGGCGGGGTTTTGTTAAAAAAGATAAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCg < 1:544519/149‑1 (MQ=255) tGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCg > 2:689805/1‑149 (MQ=255) ttttCATCGGGAAAAAACATTTTTAATACTTTATCGACCAGAGGGGGGTTTTTTTAAAAAAGATAAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAg < 1:439367/149‑1 (MQ=255) acaaaaaaaTTTTTAATACTTTATCGACAAGGGGGGGGTTTTTTTTATAAAAATAATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTac < 2:277553/143‑1 (MQ=255) aCATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAAcc > 1:552098/1‑151 (MQ=255) ttAATTCTTTATCAACCAGGGCGGGTTTTTTTTAAAAAAAATAAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCt < 1:276572/146‑1 (MQ=255) cAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAAt > 2:405738/1‑151 (MQ=255) gggggggTTTTTTAAAAAAAATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACtt < 1:79678/149‑1 (MQ=255) ggCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCgg > 2:458298/1‑151 (MQ=255) tttAAAAATATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGagag < 2:440045/149‑1 (MQ=255) | AACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCG‑TTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAG > NC_000913/3412757‑3413050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |