Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,760,384	0	A	C	46.3%	39.6 / 17.3	41	V289G (GTG→GGG)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand):  ref base A (16/6);  new base C (0/19);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 7.33e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCT  >  NC_000913/3760242‑3760533                                                                                                                                               |                                                                                                                                                      ggCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACt                                                                                                                                                <  2:492309/150‑1 (MQ=255)      ccAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGAcgcc                                                                                                                                          <  2:575128/151‑1 (MQ=255)              gcgAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGCTCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCCAACAACTTGTCGCCGGAGAAACTCACCAGACCGACGCCcgccgc                                                                                                                                    >  2:355610/1‑149 (MQ=255)                ggggttctttttACCCGCGCGTAATGCCCTCTTCAGGGGGTGGCTTGGCGGGCGGGCGATCATCTCTTTTTTCCAAACAATAATCCCTGCCTGCGCCCCGCCTAACAACTTGTCGCCGGAGAAACTCCCCAGCCTGACCCCCGCCGCAAt                                                                                                                                 <  1:362998/142‑1 (MQ=255)                        ttttATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTTCTTCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCCGACTGACGCCCCCCGCAATCAACTcc                                                                                                                          >  1:425197/1‑149 (MQ=255)                          ttATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGc                                                                                                                       <  2:285958/150‑1 (MQ=255)                              ccGCGCGTAAGGCACGCTTAAGGGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTCCCAAAAATAATCCCTGCCGGCGGCCCGCCTAACAACTTGGCGCCGGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCAt                                                                                                                  <  2:442017/151‑1 (MQ=255)                                 cgcgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTgg                                                                                                               >  1:491848/1‑151 (MQ=255)                                      aaTGCCCGCTCCAGGGGGTGGCTTGGCAGGCGGGCGACCATCTCTTTTTTCCCAACAATATTCCCTGCCGGCGGCCCGCCTAACACCTTGTCGCCGGAGAAACCCCCCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCtt                                                                                                          <  1:65183/151‑1 (MQ=255)                                           ccGCTACAGCGGGGGCCTTTGAGGGGGGGCGATCATCTTTTTTTACCCAAAAATAATCCCTCCCTGCGGCCCCCCAAACAACTTGTCGCGGGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCttt                                                                                                         <  1:46205/146‑1 (MQ=255)                                                  aGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAAATCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCg                                                                                               >  2:368850/1‑150 (MQ=255)                                                   gCGGGTGGCTTTGCGGGCGGGCGATCATCTCTTTTTTACAAACAATAATCCCGGCCTGCGGCCCGCCTAACAACTTGCCGCCGGAAAAACTCCCCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCg                                                                                               <  1:188595/149‑1 (MQ=255)                                                     gggTGGTTTTGGGGGCGGGCGACCATCTTTTTTTTCCCAAAAATACTCCCTCCCGGCGCCCCGCTTAACAACTTGTCGCCGGAGAAACCCACCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGt                                                                                              <  1:419683/148‑1 (MQ=255)                                                     gggTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTCCCAACAATAATCCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAAAACCTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGctct                                                                                                           <  1:406626/135‑1 (MQ=255)                                                     gggTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGctct                                                                                                           >  2:406663/1‑135 (MQ=255)                                                            tttGCAGGCGGGCGACCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCt                                                                                       <  2:178165/148‑1 (MQ=255)                                                                cAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTa                                                                                      >  1:252387/1‑145 (MQ=255)                                                                 aGGCGGGCGATCATCTCTTTTTTCCCAACAATAATCCCGGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAACCTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGa                                                                                   <  2:414309/147‑1 (MQ=255)                                                                 aGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCg                                                                                >  2:685113/1‑150 (MQ=255)                                                                  ggcgggcgATCTTCTCTTTTTTACAAAAAATATTCCCTGCCTGCGCCCCGCGTAACAACTTGTCGCCGGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGAc                                                                              <  1:547204/151‑1 (MQ=255)                                                                  ggcgggcgATCATCTCTTTTTTACCAAAAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGAt                                                                                  <  2:620841/147‑1 (MQ=255)                                                                     ggggtatcttcTCTTTTTTACCAAAAATAATTCCTCCCGGCGGCCCGCCTAAAAACTTGGCGCCGGAGAAACTCCCCAGACGGAAGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGa                                                                                   <  2:181033/138‑1 (MQ=255)                                                                     gggcgATCATCTCTTTTTTACCAAAAATAATTCCTGCCTGCGCCCCGCCTAAAACTTTCCCGCCGGAGAAACCCCCCAGACAGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCa                                                                            <  2:47328/150‑1 (MQ=255)                                                                       gcgATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCg                                                                         >  1:350459/1‑151 (MQ=255)                                                                              tctcTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGATcc                                                                     >  1:409422/1‑148 (MQ=255)                                                                                  tttttcCCCAAAAAAAACCCCTCCCGGCGCCCCGCTTAACACCTTGCCGCGGGAAAACCCCCCCAGACGGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACt                                                                  <  2:104447/147‑1 (MQ=255)                                                                                      ttACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAAt                                                          >  2:344617/1‑151 (MQ=255)                                                                                       tACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTATATc                                                         >  2:295449/1‑151 (MQ=255)                                                                                        aCCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAAt                                                          >  1:165017/1‑149 (MQ=255)                                                                                           aacaaAAATCCCGGCCGGGGCCCCGCCTAAAACCTGGTCGCGGGAGAACCCCCCCAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTc                                                     <  1:251330/151‑1 (MQ=255)                                                                                                taatTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAc                                                >  2:304632/1‑151 (MQ=255)                                                                                                     cctgcctgCGCCCCGCCTAACAACTTGTCGCGGGAGAACCTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGa                                            <  2:52157/150‑1 (MQ=255)                                                                                                            gggccccGCTAAACAACTTGCCGCCGGAAAACCCCCCCACACGGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAg                                    <  1:314367/147‑1 (MQ=255)                                                                                                            gcGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATcc                                      >  2:684899/1‑149 (MQ=255)                                                                                                            gcGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATcc                                      >  1:79307/1‑149 (MQ=255)                                                                                                              gccccGCATAACATCTGGCCGCGGAAAAAACCCCCCGGCCTACCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAg                                    <  1:105982/147‑1 (MQ=255)                                                                                                                ccccccaaaaattttGCCGCGGGAAAACCCCACCAGACGACCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAg                                    <  1:488028/134‑1 (MQ=255)                                                                                                                       aacaacTTGTCGCGGGAAAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGa                         <  2:395117/151‑1 (MQ=255)                                                                                                                                 cGCCGGAAAAACCCCCCAGCCTGGCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGccacca                <  2:227759/150‑1 (MQ=255)                                                                                                                                  gCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGt              >  2:406955/1‑151 (MQ=255)                                                                                                                                     ggAGAACCCCCCAAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGtt             <  2:15260/149‑1 (MQ=255)                                                                                                                                               aCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCt  >  2:662562/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                      GGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCT  >  NC_000913/3760242‑3760533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑