Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,152	T→G	L268R (CTG→CGG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,152	0	T	G	100.0%	176.3 / NA	45	L268R (CTG→CGG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/27);  total (18/27)

AAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAT  >  NC_000913/4186013‑4186296                                                                                                                                            |                                                                                                                                                 aaaCTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTcgcg                                                                                                                                       <  2:433015/151‑1 (MQ=255)         ggAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAAc                                                                                                                               >  1:310023/1‑151 (MQ=255)                 tcgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaaca                                                                                                                        <  2:48535/150‑1 (MQ=255)                 tcgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaaca                                                                                                                        <  1:396376/150‑1 (MQ=255)                  cgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaacaac                                                                                                                      <  2:655092/151‑1 (MQ=255)                  cgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaacaac                                                                                                                      <  2:36766/151‑1 (MQ=255)                              tAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGt                                                                                                          >  1:405531/1‑151 (MQ=255)                               aaCAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGt                                                                                                          <  2:504652/150‑1 (MQ=255)                                       agagTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGAt                                                                                                 <  2:290881/151‑1 (MQ=255)                                       agagTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGAt                                                                                                 <  1:76088/151‑1 (MQ=255)                                                gatCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTgcg                                                                                        >  2:199882/1‑151 (MQ=255)                                                     tGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCgg                                                                                    >  1:657614/1‑150 (MQ=255)                                                      gACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAc                                                                                  >  2:449823/1‑151 (MQ=255)                                                          gTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAcatc                                                                               >  2:646882/1‑150 (MQ=255)                                                               gCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgt                                                                          >  2:15980/1‑150 (MQ=255)                                                               gCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcg                                                                           <  2:53683/149‑1 (MQ=255)                                                                   gTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgtacgta                                                                     <  2:17300/151‑1 (MQ=255)                                                                        gccgccAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACg                                                                  <  1:348019/149‑1 (MQ=255)                                                                          cgccAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACTTATGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGaaaa                                                              <  1:6474/151‑1 (MQ=255)                                                                          ccccAGATCTGCGTCCGGTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATGGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGaaaa                                                              <  1:199934/149‑1 (MQ=255)                                                                             cAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACg                                                           >  1:556238/1‑151 (MQ=255)                                                                              aGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGt                                                          >  2:506794/1‑151 (MQ=255)                                                                                  cTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGATATCATCGTACGTAACGAAAAACGTAtgc                                                      >  1:659079/1‑151 (MQ=255)                                                                                    gCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTAtgctg                                                    <  1:556428/151‑1 (MQ=255)                                                                                     cGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTAtgctgc                                                   <  2:579454/151‑1 (MQ=255)                                                                                            tGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGc                                            >  2:142335/1‑151 (MQ=255)                                                                                             ggTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGc                                            <  1:14264/150‑1 (MQ=255)                                                                                             ggTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGc                                            <  1:244757/150‑1 (MQ=255)                                                                                             ggTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCg                                           <  2:294274/151‑1 (MQ=255)                                                                                                 ccGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTa                                        <  2:117970/150‑1 (MQ=255)                                                                                                 ccGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAg                                       <  2:515023/151‑1 (MQ=255)                                                                                                 ccGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAg                                       <  1:499235/151‑1 (MQ=255)                                                                                                       gATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGccc                                 >  2:239251/1‑151 (MQ=255)                                                                                                               tCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGAt                         >  2:396380/1‑151 (MQ=255)                                                                                                                     cgcgACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGgt                   <  1:116730/151‑1 (MQ=255)                                                                                                                     cccgACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGgt                   >  1:144733/3‑151 (MQ=255)                                                                                                                       cgACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGgtcg                 >  1:299851/1‑151 (MQ=255)                                                                                                                            tCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAAACGCCCTGCTGGATAACGGTCGTcgcg            >  1:172536/1‑151 (MQ=255)                                                                                                                               gACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTc         <  2:488891/151‑1 (MQ=255)                                                                                                                                 ccTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGt       <  1:685119/151‑1 (MQ=255)                                                                                                                                 ccTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGt       >  1:91358/1‑151 (MQ=255)                                                                                                                                    gAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTg      <  2:556275/149‑1 (MQ=255)                                                                                                                                    gAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGc     <  1:646834/150‑1 (MQ=255)                                                                                                                                     aaCGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGc     <  2:495768/149‑1 (MQ=255)                                                                                                                                      aCGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAt  >  2:62079/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                 AAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAT  >  NC_000913/4186013‑4186296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑