Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,931,668	T→G	L263W (TTG→TGG)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,931,668	0	T	G	100.0%	145.5 / NA	40	L263W (TTG→TGG)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/17);  total (23/17)

CGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTC  >  NC_000913/1931522‑1931811                                                                                                                                                   |                                                                                                                                                cGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGttt                                                                                                                                             >  1:296731/1‑151 (MQ=255)    ccATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGgt                                                                                                                                          <  1:661292/151‑1 (MQ=255)            gtgCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCt                                                                                                                                  <  1:687686/151‑1 (MQ=255)              gCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTa                                                                                                                                 <  2:21883/150‑1 (MQ=255)                    gTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCt                                                                                                                          <  1:421546/151‑1 (MQ=255)                        gTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTgtgg                                                                                                                      >  2:270374/1‑151 (MQ=255)                        gTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTgtgg                                                                                                                      >  2:132341/1‑151 (MQ=255)                          cATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTgtggt                                                                                                                     <  2:334149/150‑1 (MQ=255)                           aTCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGtga                                                                                                                   <  1:138768/151‑1 (MQ=255)                                     aGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGAtt                                                                                                         <  1:157877/151‑1 (MQ=255)                                           cGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGt                                                                                                   <  2:651852/151‑1 (MQ=255)                                            gACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTg                                                                                                  <  2:385635/151‑1 (MQ=255)                                               tACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAgg                                                                                               >  2:301052/1‑151 (MQ=255)                                                   gTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGtctc                                                                                           <  2:78809/151‑1 (MQ=255)                                                          cTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGt                                                                                    >  2:661421/1‑151 (MQ=255)                                                             gCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCca                                                                                 <  1:155558/151‑1 (MQ=255)                                                               aaCCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCcac                                                                                >  1:430335/1‑150 (MQ=255)                                                                    caGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGAt                                                                           <  2:256468/150‑1 (MQ=255)                                                                     aGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATAc                                                                         >  1:125563/1‑151 (MQ=255)                                                                      gCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATAcc                                                                        <  1:557459/151‑1 (MQ=255)                                                                      gCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATAcc                                                                        <  1:336487/151‑1 (MQ=255)                                                                      gCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATAcc                                                                        >  2:47515/1‑151 (MQ=255)                                                                                ccGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTg                                                               >  1:157056/1‑150 (MQ=255)                                                                                    ttGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGAGGATTGGGGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGtt                                                          >  1:159966/1‑151 (MQ=255)                                                                                       cccTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAAt                                                       <  2:85215/151‑1 (MQ=255)                                                                                       cccTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAAt                                                       >  2:563932/1‑151 (MQ=255)                                                                                               cGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCaa                                                <  2:201402/150‑1 (MQ=255)                                                                                                    gcAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGAtctc                                          >  2:4227/1‑151 (MQ=255)                                                                                                    gcAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGAtctc                                          >  1:95781/1‑151 (MQ=255)                                                                                                          gaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCag                                      >  1:612109/1‑149 (MQ=255)                                                                                                             tGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGtt                                  <  2:589987/150‑1 (MQ=255)                                                                                                              gCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTg                                >  2:189927/1‑151 (MQ=255)                                                                                                                  gTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCt                            >  2:669504/1‑151 (MQ=255)                                                                                                                     aaGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGc                          >  2:433837/1‑150 (MQ=255)                                                                                                                      aGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCAt                        >  2:663775/1‑151 (MQ=255)                                                                                                                           tgCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACg                   >  1:299933/1‑151 (MQ=255)                                                                                                                            gCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGt                  >  2:607625/1‑151 (MQ=255)                                                                                                                                  aCTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTc            >  2:682530/1‑151 (MQ=255)                                                                                                                                           ttATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACtt   >  1:165855/1‑151 (MQ=255)                                                                                                                                            tATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTc  >  1:619192/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                CGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTC  >  NC_000913/1931522‑1931811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑