Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	484,102	+AT	coding (302/3150 nt)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	484,098	1	.	A	100.0%	134.5 / NA	34	I102I (ATC→ATT)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (16/18);  total (16/18)
*	NC_000913	484,098	2	.	T	100.0%	133.0 / NA	34	I102I (ATC→ATA)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (16/18);  total (16/18)

CGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCG‑‑ATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTT  >  NC_000913/483954‑484239                                                                                                                                                  ||                                                                                                                                              cGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCG‑‑atatat                                                                                                                                         >  1:287893/1‑149 (MQ=255) cGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCG‑‑atata                                                                                                                                          >  1:465404/1‑149 (MQ=255)  gTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCG‑‑atatatc                                                                                                                                        <  2:702416/151‑4 (MQ=255)    cATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCG‑‑atatatccg                                                                                                                                      >  2:777906/1‑146 (MQ=255)      tGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCG‑‑atatatccgc                                                                                                                                     >  2:362773/1‑144 (MQ=255)          gCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAg                                                                                                                                  <  1:394226/151‑1 (MQ=255)            cATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGt                                                                                                                                 >  2:535497/1‑150 (MQ=255)            cATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTa                                                                                                                                <  1:400641/151‑1 (MQ=255)             aTCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTAc                                                                                                                               >  2:578796/1‑151 (MQ=255)                   gtTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACt                                                                                                                         >  2:458358/1‑151 (MQ=255)                          aCGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAg                                                                                                                    <  2:402098/149‑1 (MQ=255)                              cGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAgg                                                                                                              <  1:655367/151‑1 (MQ=255)                                    ccATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCCGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc                                                                                                        <  2:69034/151‑1 (MQ=255)                                      aTCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg                                                                                                      <  1:682583/151‑1 (MQ=255)                                                              tcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGtt                                                                              >  2:160528/1‑151 (MQ=255)                                                               cAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt                                                                               <  1:344402/149‑1 (MQ=255)                                                                   gCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAgg                                                                         >  2:716221/1‑151 (MQ=255)                                                                     tCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGAc                                                                       >  2:233865/1‑151 (MQ=255)                                                                      cACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGAc                                                                       >  1:730621/1‑150 (MQ=255)                                                                         cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGt                                                                   <  1:33972/151‑1 (MQ=255)                                                                                tgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACAt                                                               <  1:585594/148‑1 (MQ=255)                                                                                tgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACAt                                                               >  2:585646/1‑148 (MQ=255)                                                                                 gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCa                                                             <  1:160496/149‑1 (MQ=255)                                                                                    gAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGtt                                                         <  1:599876/150‑1 (MQ=255)                                                                                     aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAt                                                       <  2:314405/151‑1 (MQ=255)                                                                                                       cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCt                                     >  1:723671/1‑151 (MQ=255)                                                                                                         gCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCAACAGTCCCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTc                                      <  1:512885/148‑1 (MQ=37)                                                                                                             cGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCg                                >  1:23844/1‑150 (MQ=255)                                                                                                                  gCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAAcc                          <  1:460215/151‑1 (MQ=255)                                                                                                                     gCAGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCtg                        <  2:165949/150‑1 (MQ=255)                                                                                                                       aGTTTGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTgtgt                     <  1:370053/151‑1 (MQ=255)                                                                                                                          ttGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCAc                  >  1:700480/1‑151 (MQ=255)                                                                                                                           tGTTCTGCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCAcc                 >  2:427918/1‑151 (MQ=255)                                                                                                                                 gCACCTGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtgt             <  1:634538/149‑1 (MQ=255)                                                                                                                                      tGAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCAc      >  1:325933/1‑151 (MQ=255)                                                                                                                                       gAACCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCAc      <  1:282341/150‑1 (MQ=255)                                                                                                                                         aCCTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGt   >  1:621859/1‑151 (MQ=255)                                                                                                                                          ccTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtt  >  1:89431/1‑151 (MQ=255)                                                                                                                                          ccTGCGCGATATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtt  >  2:464170/1‑151 (MQ=255)                                                                                                                                                  ||                                                                                                                                              CGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCG‑‑ATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTT  >  NC_000913/483954‑484239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑