Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9180AC46.4% 88.2 / 14.0 56intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base A (27/3);  new base C (0/26);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 5.54e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCA  >  NC_000913/2001769‑2002067
                                                                                                                                                       |                                                                                                                                                     
aGCGGTCTAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAc                                                                                                                                                      >  2:638912/1‑151 (MQ=255)
aGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAc                                                                                                                                                      >  2:169190/1‑151 (MQ=255)
     tataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgc                                                                                                                                                 >  1:630992/1‑151 (MQ=255)
          agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTg                                                                                                                                            >  1:177919/1‑151 (MQ=255)
              aaTCCACAATAACCAGCCCGGTTTTATTTGAGTTATCGGCATGATTATCCTTTTCTGCGGGGTTTTTAATGGGACGATTGGGGGGTAAAAGGAGGGGTTATTGGGGGGTTACAGAAAAATTCCAGGCAAAAAAAAACCCCGCCGGTGGc                                                                                                                                          <  1:758771/149‑1 (MQ=255)
                      aaaaaCAGCCCTGCTTTATATGATTTATCGGCATGATTTTCCTTTTCTGCGGGTTTTTTAATCGGACGATTAGGGGGTGAAAGGGGGGTTTTTTTGGGGTTTACAGGAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCa                                                                                                                                 <  2:334849/148‑1 (MQ=255)
                              ccttGCTTTATATGTTTTACCGGCATGATTATCCTTTTCTGCGGGTTTTTAAATCGGACGATTAGGGGGAAAAATGAGGGGTTTTTGGGGGTTTAAGGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTg                                                                                                                            <  2:467137/144‑1 (MQ=255)
                              cccTGCTTTATATGAGTTATCGGCATAATTATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGGGGGTAAAAGGGGGGTTTTTTTGGGGTTTAAAGGAAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGa                                                                                                                        <  2:269650/151‑1 (MQ=255)
                              cccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                                         >  2:778239/1‑150 (MQ=255)
                                     ttATATGAGTTATCGGAATGATTATCCGTTTCGGAAGGGTTTTTAATCGGAGGATTAGTGGGTGAAAGGAGGGTTTTTTGGGGGGTTACAGGTAAATCCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                                  <  2:269063/150‑1 (MQ=255)
                                         atGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                                  >  1:765646/1‑146 (MQ=255)
                                          tGATTTATCGTCATGATTATCCTTTTCTGCAGGGTTTTTATTCGGCCAATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCt                                                                                                             <  2:206394/150‑1 (MQ=255)
                                          tGATTTATCGGCATTATTATCCGTTTCTGCGGGGTTTTTAATCGGACAATTAGTGGTTGAAAGGAGGGGTTATTGGGGGGTTAAAGTAAATTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                                 <  1:387251/146‑1 (MQ=255)
                                              ttATCGGCATGATTATCCTTTTCGGCGGGGTTTTTAATCGGACGTTTAGGGGGTAAAATGAGGGTTTTTTGGGGGGTTAAAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                           <  2:203941/148‑1 (MQ=255)
                                                       tcatttcccGTTTCTGCAGGTTTTTTAATGGGACTATTAGTGGGGGAAATGAGGGGTTTTTTGGGGGTTCCAGAAAAATCCAAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgg                                                                                                 <  2:282438/142‑1 (MQ=255)
                                                          ttaccggTTTCGGCGGGTTTTTTAATGGGACGATTGGGGGGGAAAATGAGGGGTTATTGGGGGTTTACAGGAAATTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                               <  1:114890/142‑1 (MQ=255)
                                                          ttATCCTTTTCTGCGGGTTTTTTAACCGGAGGATTAGTGGGTAAAAGGGGGGTTTATTTGGGGTTTACGGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCg                                                                                                  <  2:284756/145‑1 (MQ=255)
                                                          ttATCCTTTTCTGCGGGTTTTTTAACCGGACGTTTAGGGGGTAAAATGGGGGTTTATTGGGGGTTTACAGTAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgg                                                                                                 <  1:344131/146‑1 (MQ=255)
                                                                gTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                                       >  2:211140/1‑150 (MQ=255)
                                                                     tGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGcc                                                                                 >  2:154825/1‑151 (MQ=255)
                                                                      gCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCt                                                                                >  2:390492/1‑151 (MQ=255)
                                                                           gTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                           >  2:611422/1‑151 (MQ=255)
                                                                            ttttaaaggggACGATTAGTGGGTAAAAGGGGGGGTTATGGGGGGGTTAAAGATAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                            <  1:529584/140‑1 (MQ=255)
                                                                                 aagcGGACGATTAGTGGGTAAAAGGGGGGTTTGTTTGGGGGTTACAGTTAAATCCCAGGGAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                     <  2:126370/148‑1 (MQ=37)
                                                                                  aTCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                    >  2:762686/1‑151 (MQ=255)
                                                                                   tCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                    >  1:556556/1‑150 (MQ=255)
                                                                                     ggACGTTTGGTGGGTAAAATGAGGGTTTTTTGGGGGTTTAAAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                                 <  2:365192/151‑1 (MQ=37)
                                                                                          aTTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                             >  1:109190/1‑150 (MQ=255)
                                                                                             ggggggTAAAATGGGGGGTTTTTGGGGGTTTAAAGGTAATTCCCGGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                              <  1:279665/143‑1 (MQ=25)
                                                                                              gggggTGAAAGGAGGGGTTTTTGGGGGTTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg                                                           <  2:179903/146‑1 (MQ=37)
                                                                                                gggTAAAATGGGGGGTTTTTGGGGGTTTACAGTAAAATTCCGGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaaa                                                       <  2:370046/150‑1 (MQ=37)
                                                                                                 gggaaaaatgggggTTTTTTGGGGGTTTACAGTAAATTCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAt                                                      <  2:620184/141‑1 (MQ=25)
                                                                                                  gTGAAATGAGGGTTTATTGGGGGGTTACGTGAAAATCCCAGAAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                                    <  1:252050/151‑1 (MQ=37)
                                                                                                   tGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGTGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                                    >  2:175421/1‑150 (MQ=255)
                                                                                                   tGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                                    >  2:583166/1‑150 (MQ=255)
                                                                                                     aaaTGGGGGGTTTTTGGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCa                                                   <  2:526448/149‑1 (MQ=255)
                                                                                                        tGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa                                               >  1:44010/1‑150 (MQ=255)
                                                                                                              gTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                         >  2:161849/1‑150 (MQ=255)
                                                                                                               ttATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                                       >  2:518651/1‑151 (MQ=255)
                                                                                                               ttATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                                       >  2:53597/1‑151 (MQ=255)
                                                                                                                 aTTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                                     >  2:121716/1‑151 (MQ=255)
                                                                                                                  ttggggggTTACAGGAAATTTCCAGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                      <  2:732150/146‑1 (MQ=255)
                                                                                                                  ttggggggTTACAGGAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                      <  2:305004/146‑1 (MQ=255)
                                                                                                                      ggggTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACtt                                >  1:577781/1‑151 (MQ=255)
                                                                                                                       gggTTACAGTAAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                               <  1:346689/151‑1 (MQ=37)
                                                                                                                         gTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                              >  2:429872/1‑150 (MQ=255)
                                                                                                                            tACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGACGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                           >  2:16589/1‑151 (MQ=255)
                                                                                                                             aCAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc                          >  1:338725/1‑151 (MQ=255)
                                                                                                                             aCAGGAAAATTCAGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                            <  1:226263/149‑1 (MQ=37)
                                                                                                                              cAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc                         >  2:546296/1‑151 (MQ=255)
                                                                                                                                   aaaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt                     >  1:590586/1‑150 (MQ=255)
                                                                                                                                   aaaTTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt                     <  2:561174/150‑1 (MQ=255)
                                                                                                                                   aaaTACAAGACAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGa                      <  1:237798/149‑1 (MQ=255)
                                                                                                                                    aattcccAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg                       <  2:765730/143‑1 (MQ=255)
                                                                                                                                     aTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa                    >  2:685174/1‑149 (MQ=255)
                                                                                                                                       tcccAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag                  <  2:148313/148‑1 (MQ=255)
                                                                                                                                                aaaaaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt        <  1:264588/150‑1 (MQ=255)
                                                                                                                                                 aaaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATc       <  2:89107/151‑1 (MQ=255)
                                                                                                                                                   aaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATc       >  1:172074/1‑149 (MQ=255)
                                                                                                                                                      aaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc   >  2:401796/1‑150 (MQ=255)
                                                                                                                                                       aCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCa  >  1:384277/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
AGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCA  >  NC_000913/2001769‑2002067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: