Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,947,8500CT44.0% 55.6 / 29.6 50E40K (GAA→AAA) hdfRflhDC operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (6/22);  new base T (22/0);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 5.24e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAT  >  NC_000913/3947705‑3948000
                                                                                                                                                    |                                                                                                                                                       
tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGGAAAGGTTCCCAACCAGGTGGATTTTCAGtt                                                                                                                                                    >  1:596434/1‑151 (MQ=255)
tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTCACACCCCGGTGCTTTTTCCGtt                                                                                                                                                    >  2:635981/1‑151 (MQ=255)
 gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTTATACCCCGTTGTTTTTTCAGtt                                                                                                                                                    >  1:501033/1‑150 (MQ=255)
 gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGGAAAAGGTCACACCCCGTTGATTTTTCTGtt                                                                                                                                                    >  1:772623/1‑150 (MQ=255)
 gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTCCCACCCCGTTGGTTTTTCTGttt                                                                                                                                                   >  1:677867/1‑150 (MQ=255)
                  aCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTCACACCCCGTTGGTTTTTCCGTTTTTTGTTTCGaaa                                                                                                                                       >  2:328879/1‑146 (MQ=255)
                    ctcctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTTACACCCCGGTGATTTTTCCGTTGTTTTATTTGAAAACTCa                                                                                                                                  >  2:194495/1‑149 (MQ=255)
                        ttACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCACATGATTTTTCCAGTTTTTGATTCCAAAGCTCCctgc                                                                                                                              >  2:93813/1‑149 (MQ=255)
                                          gTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCg                                                                                                          <  1:175295/151‑1 (MQ=255)
                                             cTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCACACCCACTTGATTTTTCTGTTTTTTTATTTGAAAACTCACTGCTGACTGGGGCAGaaaaaacgaat                                                                                                       >  1:749280/1‑141 (MQ=255)
                                                       tttCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTCACACCCAGGTGGTTTTTCCGTTGGCTGATTCGAAAGATCACTGCTGACTGGGGCCGATAGAGCGATTCAGCCGCTc                                                                                              >  2:273168/1‑150 (MQ=255)
                                                                aaGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGTTCACACCCAGTTGATTTTTCAGTTGTCTTATTTGAAAGATCACTGCTGACTGGGTCAGATAGAGCGATTTAGCCGCCCGACCaaa                                                                                      >  2:713985/1‑148 (MQ=255)
                                                                  ggCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGAc                                                                                 <  1:139476/151‑1 (MQ=255)
                                                                    cAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGTTCACACCCCGTTGATTTTTCCGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGGCCGATAGAGGGATTTAGCCGCTCCACCAAAggg                                                                                   >  2:303249/1‑145 (MQ=255)
                                                                      gtagtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGTTCACACCCACTTGATTTTTTAGTTTTTTGATTCGCAAAATCACTGCTGACTTGGGGCGATAGAGCGAGTTAGCCGCCCCACCaaaacgaccc                                                                               >  1:523626/1‑142 (MQ=255)
                                                                        agtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGtt                                                                            <  1:778972/150‑1 (MQ=255)
                                                                        agtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTc                                                                           <  1:378026/151‑1 (MQ=255)
                                                                         gtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGTAAAAGGTCACACCCCCCTGATTTTTCAGTTTTCTTGTTTGAAAGATCACTGCTGACGGGGGCCGATAAAGCGCTTCAGCCGCCCGACCAAAAAGAcgcg                                                                              >  1:183173/1‑147 (MQ=255)
                                                                          tttttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTTTAAAAGGGCTGACCCCGATTATTTTTCTAGTGGCTGTTTCGAAAGATAACCGCTTACTGGGGGAGGTAAAGAGGTTGAAACGCCCGAACAAAATGGcgcggtggg                                                                         >  1:567503/1‑146 (MQ=255)
                                                                          tttttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTCACACCCAGTTGATTTTTCAGTTGTTTTGTTCGAAAACTCACTTCTGACTGGGTCAGATAAAGCGATTCAGCCCCCCGACCAAAAGGACGCGTTTgg                                                                         >  2:668188/1‑151 (MQ=255)
                                                                             ttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCt                                                                       <  2:530489/150‑1 (MQ=255)
                                                                              tCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTa                                                                      <  2:174176/150‑1 (MQ=255)
                                                                               cACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGGAAAAGGTCGCACCCCCTTGTTTTTCCTGTTTTCTGGTTTGGAAGCTCACCGCTGACTGGGTCAGATAGAACGATTCAAGCCCTCGACCCAAGGGAAGCGTTTgg                                                                         >  1:756663/1‑146 (MQ=255)
                                                                                  cGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGTTCACACCCAGTTGATTTTTCCGTTTTCTGATTTGAAAACTCCCTGCTGACTGGGTCCGATAGAGCGATTCAGCCCCTCGACCAAAGGGACGCGTTCGGCTAACtt                                                                  >  2:416957/1‑150 (MQ=255)
                                                                                      aGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGGAAAAGGTTACACCCCGATGATTTTTCAGTTTTTTGATTCTAAAACTCACTGCTGACTGGGTGAGATAGAGCGATTTCGCCGCTCGACCCCAATGAGGCGGTCGGGTAACTTCCAgg                                                             >  1:546734/1‑151 (MQ=255)
                                                                                         ggTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGaaa                                                          <  2:550950/151‑1 (MQ=255)
                                                                                         ggTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGTTTACCCCCAGTTTGTTTTTCAGTTGTTTGGTTCGAAAACTCACTGCTGACTGGGGCCGATAGAGCGATTTAGCCGCTCGACCCAAATGACGCGTTTGGCTGACTTCCAGGaa                                                           >  2:233472/1‑150 (MQ=255)
                                                                                            tAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACCCCCAGGTGATTTTTCCGTTGTCTTTTTCGAAAGCTCACCGCTGACTGGGGTAGATAAAGCGGTTAAACCCCCCGATCAAAGAGACGGGTTTGGCTAACTTCCCGGaa                                                           >  2:322771/1‑147 (MQ=255)
                                                                                             aaaCGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCACACCCAGTTGGTTTTTCAGTTTTTTTATTTGAAAACTCACTGCTGACTGGGTCAGATAAAGCGATTCAGCCGCTCGACCCAAGTGAAGCGTTCGGCTTACTTCCCGGAAAGgtt                                                      >  2:92501/1‑151 (MQ=255)
                                                                                                 ggATATTGTTTCTGTGGCGGGTGAAAAGGTTCACGCCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGATGACTGGGTCAGATAGAGCGATTCAGCCGCTAGCCCAAAGTGACGCGTTCGGCTAACTTCCAGAAAAGTTTTTaa                                                  <  1:731878/151‑1 (MQ=255)
                                                                                                 ggATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTaa                                                  <  2:284284/151‑1 (MQ=255)
                                                                                                 ggATATTGTTTCTGTGGCGGGTGAAAAAGGTCACACCCAGGTGATTTTTCAGTTGTCTGATTCGAAAACTCACTGCTGGCTGGGGCAGATAGAGCGATTCAGCCGCTCGACCAAAGAGACGCGTTCGGCTAACTTCCAGGGAAGttttt                                                    >  2:665120/1‑149 (MQ=255)
                                                                                                   atatTGTTTCTGTGGCGGGTGAAAAGGGTGCCACCCCGGTGTTTTTTCCGTTTTTTGGTTGGGAAGCTCCATTCTGACTGGGGCAGATAGAGCGATTTAGCCGCCCGACCCAAAGGGAGCGGTTTGGTTACTTCCAGGAAAGttttt                                                    >  1:751571/1‑147 (MQ=255)
                                                                                                           tCTGTGGCGGGTGAAAAGGTTCTCTCCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGGCTGGGTCAGATAGAGCGATACAGCCGCTCGACCAAAGTGACGCGTTCGGCTATCTTCCAGGAAAGTTTTTAACAATTCCg                                          <  1:675163/149‑1 (MQ=255)
                                                                                                           tCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGt                                         <  1:225798/150‑1 (MQ=255)
                                                                                                              gtgGCGGGTGAAAAGGTTCACTCCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTAt                                       <  1:731241/149‑1 (MQ=255)
                                                                                                                    gggTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGt                                <  1:25936/151‑1 (MQ=255)
                                                                                                                            aaGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTcc                         >  1:774455/1‑151 (MQ=255)
                                                                                                                               gTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCa                      <  2:361314/151‑1 (MQ=255)
                                                                                                                               gTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCa                      <  1:177732/151‑1 (MQ=255)
                                                                                                                                  cacaCCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCaaaa                   <  1:15188/151‑1 (MQ=255)
                                                                                                                                       ccAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAattatt              <  1:751911/151‑1 (MQ=255)
                                                                                                                                       ccAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAattatt              >  1:232045/1‑151 (MQ=255)
                                                                                                                                        cAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTAttt             <  1:291818/151‑1 (MQ=255)
                                                                                                                                         aGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTg            <  2:289281/151‑1 (MQ=255)
                                                                                                                                             gATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCg         <  2:731402/150‑1 (MQ=255)
                                                                                                                                                ttttCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGtt       >  1:562998/1‑150 (MQ=255)
                                                                                                                                                  ttCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTAt     <  1:462123/150‑1 (MQ=255)
                                                                                                                                                  ttCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATg    >  1:629621/1‑151 (MQ=255)
                                                                                                                                                   tCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATg    <  1:204625/150‑1 (MQ=255)
                                                                                                                                                    ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGa   <  2:137741/150‑1 (MQ=255)
                                                                                                                                                    ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGa   <  1:157092/150‑1 (MQ=255)
                                                                                                                                                    ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAt  >  2:222341/1‑151 (MQ=255)
                                                                                                                                                    ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAt  <  1:343587/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAT  >  NC_000913/3947705‑3948000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: