Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,947,850 | 0 | C | T | 44.0% | 55.6 / 29.6 | 50 | E40K (GAA→AAA) | hdfR | flhDC operon transcriptional repressor |
Reads supporting (aligned to +/- strand): ref base C (6/22); new base T (22/0); total (28/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.24e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAT > NC_000913/3947705‑3948000 | tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GGAAAGGTTCCCAACCAGGTGG‑ATTTTCAGtt > 1:596434/1‑151 (MQ=255) tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GGAAAAGGTCACACCCCGGTGC‑TTTTTCCGtt > 2:635981/1‑151 (MQ=255) gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGG‑GAAAAGGTT‑ATACCCCGTTGT‑TTTTTCAGtt > 1:501033/1‑150 (MQ=255) gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GGAAAAGGTCACACCCCGTTGA‑TTTTTCTGtt > 1:772623/1‑150 (MQ=255) gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GGAAAAGGTCCCACCCCGTTGG‑TTTTTCTGttt > 1:677867/1‑150 (MQ=255) aCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GGAAAAGGTCACACCCCGTTGG‑TTTTTCCGTTTTTTGTTTCGaaa > 2:328879/1‑146 (MQ=255) ctcctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GAAAAAGGTTACACCCCGGTGA‑TTTTTCCGTTGTTTTATTTGAAAACTCa > 2:194495/1‑149 (MQ=255) ttACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GAAAAGGGTCACACCCACATGATTTTTCCAGTT‑TTTGATTCCAAAGCTCCctgc > 2:93813/1‑149 (MQ=255) gTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCg < 1:175295/151‑1 (MQ=255) cTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GAAAAAGGTCACACCCACTTGA‑TTTTTCTGTTTTTTTATTTGAAAACTCACTGCTGACTGGGGCAGaaaaaacgaat > 1:749280/1‑141 (MQ=255) tttCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAAGGTCACACCCAGGTGG‑TTTTTCCGTTGGCTGATTCGAAAGATCACTGCTGACTGGGGCCGATAGAGCGATTCAGCCGCTc > 2:273168/1‑150 (MQ=255) aaGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GAAAAAGTTCACACCCAGTTGA‑TTTTTCAGTTGTCTTATTTGAAAGATCACTGCTGACTGGGTCAGATAGAGCGATTTAGCCGCCCGA‑CCaaa > 2:713985/1‑148 (MQ=255) ggCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGAc < 1:139476/151‑1 (MQ=255) cAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GAAAAAGTTCACACCCCGTTGA‑TTTTTCCGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGGCCGATAGAGGGATTTAGCCGCTCCA‑CCAAAggg > 2:303249/1‑145 (MQ=255) gtagtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAAGTTCACACCCACTTGA‑TTTTTTAGTTTTTTGATTCGCAAAATCACTGCTGACTTGGGGCGATAGAGCGAGTTAGCCGCCCCA‑CCaaaacgaccc > 1:523626/1‑142 (MQ=255) agtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGtt < 1:778972/150‑1 (MQ=255) agtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTc < 1:378026/151‑1 (MQ=255) gtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GTAAAAGGTCACACCCCCCTGA‑TTTTTCAGTTTTCTTGTTTGAAAGATCACTGCTGACGGGGGCCGATAAAGCGCTTCAGCCGCCCGA‑CCAAAAAGAcgcg > 1:183173/1‑147 (MQ=255) tttttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑TTAAAAGGGCTGACCCCGATTA‑TTTTTCTAGTGGCTGTTTCGAAAGATAACCGCTTACTGGGGGAGGTAAAGAGGTTGAAACGCCCGA‑ACAAAATGGcgcggtggg > 1:567503/1‑146 (MQ=255) tttttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAAGGTCACACCCAGTTGA‑TTTTTCAGTTGTTTTGTTCGAAAACTCACTTCTGACTGGGTCAGATAAAGCGATTCAGCCCCCCGA‑CCAAAAGGACGCGTTTgg > 2:668188/1‑151 (MQ=255) ttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCt < 2:530489/150‑1 (MQ=255) tCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTa < 2:174176/150‑1 (MQ=255) cACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GGAAAAGGTCGCACCCCCTTGT‑TTTTCCTGTTTTCTGGTTTGGAAGCTCACCGCTGACTGGGTCAGATAGAACGATTCAAGCCCTCGA‑CCCAAGGGAAGCGTTTgg > 1:756663/1‑146 (MQ=255) cGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GAAAAAGTTCACACCCAGTTGA‑TTTTTCCGTTTTCTGATTTGAAAACTCCCTGCTGACTGGGTCCGATAGAGCGATTCAGCCCCTCGA‑CCAAAGGGACGCGTTCGGCTAACtt > 2:416957/1‑150 (MQ=255) aGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGGAAAAGGTT‑ACACCCCGATGA‑TTTTTCAGTTTTTTGATTCTAAAACTCACTGCTGACTGGGTGAGATAGAGCGATTTCGCCGCTCGA‑CCCCAATGAGGCGGTCGGGTAACTTCCAgg > 1:546734/1‑151 (MQ=255) ggTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGaaa < 2:550950/151‑1 (MQ=255) ggTTAAACGGATATTGTTTCTGTGGC‑GGGG‑GAAAAAGTTTACCCCCAGTTTG‑TTTTTCAGTTGTTTGGTTCGAAAACTCACTGCTGACTGGGGCCGATAGAGCGATTTAGCCGCTCGA‑CCCAAATGACGCGTTTGGCTGACTTCCAGGaa > 2:233472/1‑150 (MQ=255) tAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAAGGTTACCCCCAGGTGA‑TTTTTCCGTTGTCTTTTTCGAAAGCTCACCGCTGACTGGGGTAGATAAAGCGGTTAAACCCCCCGA‑TCAAAGAGACGGGTTTGGCTAACTTCCCGGaa > 2:322771/1‑147 (MQ=255) aaaCGGATATTGTTTCTGTGGC‑GGGG‑GAAAAAGGTCACACCCAGTTGG‑TTTTTCAGTTTTTTTATTTGAAAACTCACTGCTGACTGGGTCAGATAAAGCGATTCAGCCGCTCGA‑CCCAAGTGAAGCGTTCGGCTTACTTCCCGGAAAGgtt > 2:92501/1‑151 (MQ=255) ggATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACGCCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGATGACTGGGTCAGATAGAGCGATTCAGCCGCTAGC‑CCAAAGTGACGCGTTCGGCTAACTTCCAGAAAAGTTTTTaa < 1:731878/151‑1 (MQ=255) ggATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTaa < 2:284284/151‑1 (MQ=255) ggATATTGTTTCTGTGGC‑GGGT‑GAAAAAGGTCACACCCAGGTGA‑TTTTTCAGTTGTCTGATTCGAAAACTCACTGCTGGCTGGGGCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGAGACGCGTTCGGCTAACTTCCAGGGAAGttttt > 2:665120/1‑149 (MQ=255) atatTGTTTCTGTGGC‑GGGT‑GAAAAGGGTGCCACCCCGGTGT‑TTTTTCCGTTTTTTGGTTGGGAAGCTCCATTCTGACTGGGGCAGATAGAGCGATTTAGCCGCCCGACCCAAAGGGA‑GCGGTTTGGTTACTTCCAGGAAAGttttt > 1:751571/1‑147 (MQ=255) tCTGTGGC‑GGGT‑GAAAAGGTTCTCTCCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGGCTGGGTCAGATAGAGCGATACAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTATCTTCCAGGAAAGTTTTTAACAATTCCg < 1:675163/149‑1 (MQ=255) tCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGt < 1:225798/150‑1 (MQ=255) gtgGC‑GGGT‑GAAAAGGTTCACTCCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTAt < 1:731241/149‑1 (MQ=255) gggT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGt < 1:25936/151‑1 (MQ=255) aaGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTcc > 1:774455/1‑151 (MQ=255) gTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCa < 2:361314/151‑1 (MQ=255) gTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCa < 1:177732/151‑1 (MQ=255) cacaCCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCaaaa < 1:15188/151‑1 (MQ=255) ccAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAattatt < 1:751911/151‑1 (MQ=255) ccAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAattatt > 1:232045/1‑151 (MQ=255) cAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTAttt < 1:291818/151‑1 (MQ=255) aGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTg < 2:289281/151‑1 (MQ=255) gA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCg < 2:731402/150‑1 (MQ=255) ttttCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGtt > 1:562998/1‑150 (MQ=255) ttCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTAt < 1:462123/150‑1 (MQ=255) ttCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATg > 1:629621/1‑151 (MQ=255) tCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATg < 1:204625/150‑1 (MQ=255) ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGa < 2:137741/150‑1 (MQ=255) ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGa < 1:157092/150‑1 (MQ=255) ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAt > 2:222341/1‑151 (MQ=255) ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAt < 1:343587/151‑1 (MQ=255) | TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGT‑GAAAAGGTTCACACCCAGTTGA‑TTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGA‑CCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAT > NC_000913/3947705‑3948000 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |