Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,043,030	0	A	G	52.9%	13.4 / 22.0	17	N206D (AAT→GAT)	srkA	Cpx stress response Thr/Ser protein kinase; MazF antagonist protein
Reads supporting (aligned to +/- strand):  ref base A (3/5);  new base G (9/0);  total (12/5)
Fisher's exact test for biased strand distribution p-value = 9.05e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04

CAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTAT  >  NC_000913/4042885‑4043170                                                                                                                                                  |                                                                                                                                             cAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGGTTTTACCGTTCTTCGGCTACCTTGAGGATGCCCCGCCGGGGGTAtt                                                                                                                                         >  1:666309/1‑151 (MQ=255)  aaGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATtc                                                                                                                                        <  1:236849/151‑1 (MQ=255)    gCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATtctc                                                                                                                                      <  2:641010/151‑1 (MQ=255)       gTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTg                                                                                                                                    <  1:381689/150‑1 (MQ=255)                         gATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAAtgtt                                                                                                                  <  2:211330/150‑1 (MQ=255)                               ttCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTCTGCGGCCACATGGAGAATGCCACGCCGGGGATATTTTTTGGCGCGGTGGGCCCATGTTTGTTGAt                                                                                                           >  2:441859/1‑151 (MQ=255)                               ttCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTCACCGTTCTGCGGGGACAAGGAGACTGGCACGCCGGGGATATTTTCTGGCGCGGTGGGCCAATGTTTGTTGAt                                                                                                           >  2:674172/1‑151 (MQ=255)                                  cGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCGCATAGAGACTGCCCCGCCGGGGATATTCTCTTGCGCGCTGGTCCAATGTTTTTTGATCt                                                                                                         >  1:731496/1‑150 (MQ=255)                                    ggTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGGGAATTCACCCGTCTGGGGCGACATGGCGGCTGCCACGCCCGGGATTTTCTCTTGCCCGGTTGGCTGATGTTTTTTGATCTg                                                                                                        >  1:95552/1‑149 (MQ=255)                                       tGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCCTTCTGCGGCTACATGGAGACTGCCCCCCCGGGGATATTTTCTGGCGCGATGGGCCAATGTTTGTTGATCTgg                                                                                                       >  2:683597/1‑147 (MQ=255)                                          aaGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGCTGTGACCGTTCTGCCGCTACACGGAGACGGCCCCGCCCCGGACATTTTCTGGTGCGTGGGGCCAACGTTTGTTGTTGTGgatta                                                                                                   >  1:595308/1‑146 (MQ=255)                                              ggCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACg                                                                                             >  2:280036/1‑150 (MQ=255)                                                                            tGCCGCCGTTACAGCACACTGGCGGGGAGATTTTCCCGGTCTGCGGCTACATGGGGAATGCCACCCCGGGGATATTCTCTGGCGCGGTGGTCCCATGTTTGTTGTTCTGGGTGATGCCCGCAATGGGCCAGCCGGTCAGGAttt                                                                     >  1:28875/1‑144 (MQ=255)                                                                                                             cACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATg                             <  1:591763/151‑1 (MQ=255)                                                                                                                             cATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACtatt              >  2:57480/1‑150 (MQ=255)                                                                                                                                        ccACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTAt  >  2:534947/1‑151 (MQ=255)                                                                                                                                        ccACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTAt  >  1:273227/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                             CAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTAT  >  NC_000913/4042885‑4043170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑