Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	718,659	0	T	A	43.9%	66.3 / 14.8	41	D601V (GAT→GTT)	speF	ornithine decarboxylase isozyme, inducible
Reads supporting (aligned to +/- strand):  ref base T (1/22);  new base A (18/0);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 9.40e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAG  >  NC_000913/718515‑718807                                                                                                                                                 |                                                                                                                                                     tCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATg                                                                                                                                                  <  1:692797/149‑1 (MQ=255)    gCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCAttt                                                                                                                                             <  1:380238/151‑1 (MQ=255)      aTCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCAttt                                                                                                                                             >  1:202993/1‑149 (MQ=255)        ccGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCt                                                                                                                                          <  1:615074/150‑1 (MQ=255)              gACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTac                                                                                                                                       >  2:712379/1‑147 (MQ=255)                   aCCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGAc                                                                                                                              <  2:389974/151‑1 (MQ=255)                    ccAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTACAAAAAATGCAttttttttcacaa                                                                                                                                   >  1:732837/1‑135 (MQ=255)                     cAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATTCATTTTCTTACACAAccg                                                                                                                                >  2:561694/1‑145 (MQ=255)                               tCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAAAGCATTTTCTTACACAAAAGACGCAgggggaaa                                                                                                                   >  2:422185/1‑145 (MQ=255)                                 aCCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCg                                                                                                                <  2:385785/151‑1 (MQ=255)                                  ccGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACACCTGACGCAGGGTATAACCgg                                                                                                               <  1:798016/151‑1 (MQ=255)                                  ccGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCg                                                                                                                <  2:604993/150‑1 (MQ=255)                                       tAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACAACTGACGCCGGGGATAACCGGCg                                                                                                             >  2:702715/1‑148 (MQ=255)                                         aaTAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATAAAAAATAAGCATTTCCCTACACAACTGACGCAGGGgattaacag                                                                                                               >  2:236359/1‑138 (MQ=255)                                            aGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGctc                                                                                                     <  2:312361/151‑1 (MQ=255)                                             ggCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAAAATGCCTTTTCTGTCACAAATGACGCAGGGGGTAAACgggggggggctc                                                                                                     >  1:212058/1‑140 (MQ=255)                                                gTAGTTGGCTTCTTGCGGGTTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGGTAAAAAAAATGCATTTTCTTACACAAAAGACGCAGGGGATAAACGGCGGAGCGccct                                                                                                    >  1:140433/1‑148 (MQ=255)                                                gTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACAAGCATTTTCTTACACAACTGACGCCGGGGATAAACCGCGTAGCGCTCtt                                                                                                   >  2:581895/1‑149 (MQ=255)                                                 tAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAATTCATTTTTTTACACAACAGAGGCCGGGGATAAACCGCGTAGGGCTCtt                                                                                                   >  2:795728/1‑148 (MQ=255)                                                          tcttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGttt                                                                                         <  2:238513/149‑1 (MQ=255)                                                          tcttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTg                                                                                        <  1:232217/150‑1 (MQ=255)                                                             tGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTa                                                                                      <  2:732920/149‑1 (MQ=255)                                                              gCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGAt                                                                                   <  1:355235/151‑1 (MQ=255)                                                                ggATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATgt                                                                                 <  1:670376/151‑2 (MQ=255)                                                                  aTTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATAAAAAACATGCATTTTCTTATCAAAATCACGCAGGGGATAAACCGCGGAGGGCCCTTTTTTCTGGTTGTtggtgg                                                                                 >  1:383302/1‑149 (MQ=255)                                                                            aCGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACtt                                                                     <  1:426714/151‑1 (MQ=255)                                                                              gcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCt                                                                   <  1:330393/151‑1 (MQ=255)                                                                                       gTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGgc                                                          <  2:494196/151‑1 (MQ=255)                                                                                              ttACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCCTTTCCTGACACAAATGACGCCGGGGGAAAACCGCGGAGCGCGCTTCCTGATGTTTTTGTATGGGAGGGAGGACCTTTGCTAATggcggggcgg                                                   >  2:662475/1‑151 (MQ=255)                                                                                                aCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATCCATTTTCTGACACAACATACGCAGGGGGTAAACCGCGGATGGGGCTTCATGCTGTTTGTTAAAGGGAGGGAGCAccttctctaaaaggg                                                         >  1:611167/1‑129 (MQ=255)                                                                                                aCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGa                                                  <  2:57527/150‑1 (MQ=255)                                                                                                aCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAc                                                 <  2:630650/151‑1 (MQ=255)                                                                                                     aCATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAATATGTATTTTCTTTCCCAACTTACGCCGGGGGTAAACGGCGGAGGGCTCTTTCTTCTGTTTTTTGAGGGAAGGGAGCACTTCTGTTAATGGGGCGTCggg                                                  >  1:218744/1‑144 (MQ=255)                                                                                                              tttGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGttttt                                    <  2:755373/150‑1 (MQ=255)                                                                                                                 gCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTCCTGACACAACTGACCGCGGGGGTAAACCGCGGAGGGCTCTTCCTTCTGTTTTTAGATGGGAGGGAGCACCTCTTCTAATGGCGCGGCGGGCTCCAGCAGgtttt                                    >  1:586287/1‑147 (MQ=255)                                                                                                                     ttgttTCACGTTGTGGCGGGCCTAAAAAACATACACTTCCTTTCCCAACACACCGAGGGGGTAAACGGGGGAGCGCCCCTCATTCTGTTTGTTGGTGGGAGGGAGGAACTCTTCTTAAGGCGCGTCGGGCCCAAACAGttttttgt                                 >  1:3181/1‑143 (MQ=255)                                                                                                                      tgttTCACGTTGTGGCGGGCATACAAAAAATGCATTTTCTGTCACAACACAAGCAGGGGGTAAACGGCGGAGCGCCCTTCTTTCTTTTTTTTGATGGGAGGGAGCACTTCTTCTCATGGGGCGGCGGGCCCCAACAGgttttttaaggg                             >  1:67100/1‑142 (MQ=255)                                                                                                                          tCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACca                        <  2:470190/150‑1 (MQ=255)                                                                                                                                       ggCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGt          <  1:624875/151‑1 (MQ=255)                                                                                                                                          aTACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGAGTCGGACTCAAGCAGGTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGc       >  1:659555/1‑151 (MQ=255)                                                                                                                                               aaTCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAg  <  2:235550/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     TCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAG  >  NC_000913/718515‑718807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑