Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,466,841 IS5 (+) +4 bp coding (403‑406/948 nt) treR ← trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2288919 =NA (NA)61 (1.100) 44/298 0.5 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 4466841 = 0 (0.000)coding (406/948 nt) treR trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor
* ? NC_000913 = 2290113NA (NA)65 (1.170) 47/298 0.3 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 4466844 0 (0.000)coding (403/948 nt) treR trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor

Only 100 of 115 total aligned reads displayed.

TGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTGGGTCGTAACCTTGTTCATAGAACGCTG  >  NC_000913/4466691‑4466990
                                                                                                                                                      |                                                                                                                                                     
ctaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccc                                                                                                                                                       >  1:779555‑M2/151‑151 (MQ=255)
 taaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                                      >  2:643328‑M2/150‑151 (MQ=255)
 taaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                                      >  2:43247‑M2/150‑151 (MQ=255)
 taaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                                      >  1:403837‑M2/150‑151 (MQ=255)
  aaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                                      >  2:176865‑M2/149‑150 (MQ=255)
  aaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                                      <  1:835272‑M2/2‑1 (MQ=255)
  aaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTa                                                                                                                                                     <  2:627056‑M2/3‑1 (MQ=255)
   aaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTaa                                                                                                                                                    <  1:166982‑M2/4‑1 (MQ=255)
   aaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTaa                                                                                                                                                    <  1:595771‑M2/4‑1 (MQ=255)
   aaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTa                                                                                                                                                     >  2:271837‑M2/148‑150 (MQ=255)
       ctggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACAtt                                                                                                                                                >  2:668851‑M2/144‑151 (MQ=255)
       cACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAgga                                                                                                                                                 <  1:576663‑M2/150‑4 (MQ=255)
         ggggctaacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATttc                                                                                                                                              >  1:675240‑M2/142‑151 (MQ=255)
          gggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATttct                                                                                                                                             <  1:565528‑M2/11‑1 (MQ=255)
              agaTAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcga                                                                                                                                         >  1:601679‑M2/1‑140 (MQ=255)
               gaTAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcga                                                                                                                                         <  2:339354‑M2/150‑12 (MQ=255)
               aacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGt                                                                                                                                        >  2:178714‑M2/136‑151 (MQ=255)
                   ccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTAt                                                                                                                                     <  1:87108‑M2/19‑1 (MQ=255)
                   ccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATg                                                                                                                                    <  1:492676‑M2/20‑1 (MQ=255)
                   ccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATg                                                                                                                                    <  2:682132‑M2/20‑1 (MQ=255)
                       aaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATgccg                                                                                                                                 <  2:148634‑M2/23‑2 (MQ=255)
                       aaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGcc                                                                                                                                  >  2:581020‑M2/128‑149 (MQ=255)
                       aaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCa                                                                                                                                 <  1:89413‑M2/23‑1 (MQ=255)
                          ttACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctg                                                                                                                              <  1:4927‑M2/150‑23 (MQ=255)
                           ggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTaa                                                                                                                             <  2:76969‑M2/27‑1 (MQ=255)
                             cgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACAttt                                                                                                                                               >  2:39890‑M2/122‑130 (MQ=255)
                             cgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACAttt                                                                                                                                               <  1:39858‑M2/9‑1 (MQ=255)
                             cGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatat                                                                                                                          <  2:471091‑M2/151‑27 (MQ=255)
                               aTGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatg                                                                                                                         <  2:728459‑M2/150‑28 (MQ=255)
                                  aaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAAc                                                                                                                     >  1:88326‑M2/117‑151 (MQ=255)
                                    ccTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatc                                                                                                                    <  1:545712‑M2/150‑33 (MQ=255)
                                         tCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgttt                                                                                                              <  1:671331‑M2/151‑39 (MQ=255)
                                         tCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgttt                                                                                                              <  2:613125‑M2/151‑39 (MQ=255)
                                          taaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTAc                                                                                                             >  2:750647‑M2/109‑151 (MQ=255)
                                          cATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttg                                                                                                             <  1:485703‑M2/151‑40 (MQ=255)
                                            aataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGc                                                                                                           >  1:625919‑M2/107‑151 (MQ=255)
                                             aCAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtc                                                                                                           >  1:440407‑M2/1‑109 (MQ=255)
                                             aCAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtc                                                                                                           >  2:597461‑M2/1‑109 (MQ=255)
                                               aggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCg                                                                                                         <  2:338945‑M2/47‑1 (MQ=255)
                                               aGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatc                                                                                                        <  1:63800‑M2/151‑45 (MQ=255)
                                                ggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTc                                                                                                       <  2:152390‑M2/49‑1 (MQ=255)
                                                      ttcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATAtt                                                                                                 >  1:344171‑M2/97‑151 (MQ=255)
                                                      gCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagc                                                                                                  >  2:325872‑M2/1‑100 (MQ=255)
                                                       cATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagcca                                                                                                >  2:695570‑M2/1‑99 (MQ=255)
                                                         aaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACg                                                                                              <  1:301212‑M2/58‑1 (MQ=255)
                                                           aGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccataga                                                                                            >  1:613059‑M2/1‑95 (MQ=255)
                                                            ggATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccataga                                                                                            <  2:680001‑M2/150‑57 (MQ=255)
                                                              atttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgaggcagccgagaagaatgcccctgcttatccgcacctcccCTAGCATTTCCTCTGTTATGCCTGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGtt                                                                                         <  1:589847‑M2/63‑1 (MQ=255)
                                                                       gCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcat                                                                                 >  2:4936‑M2/1‑83 (MQ=255)
                                                                         aaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTccc                                                                              >  1:745293‑M2/78‑151 (MQ=255)
                                                                         aaaaaaacggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccgtccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTccc                                                                              <  1:459802‑M2/74‑1 (MQ=255)
                                                                          ccTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatg                                                                             >  1:187013‑M2/1‑80 (MQ=255)
                                                                          ccTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatg                                                                             <  1:824286‑M2/151‑72 (MQ=255)
                                                                            aaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCaaa                                                                           <  2:429618‑M2/77‑1 (MQ=255)
                                                                              atcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATg                                                                         <  1:657700‑M2/79‑1 (MQ=255)
                                                                               gtcgtcATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtc                                                                         <  2:859014‑M2/150‑76 (MQ=255)
                                                                                 ggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTc                                                                      <  2:471116‑M2/82‑1 (MQ=255)
                                                                                 cgtcATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatc                                                                      <  2:8581‑M2/151‑79 (MQ=255)
                                                                                     aTAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaact                                                                  >  1:185514‑M2/1‑69 (MQ=255)
                                                                                     aTAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttggcatctggagccatagaacagggttcatcatgagtcatcaac                                                                   <  1:761140‑M2/150‑82 (MQ=255)
                                                                                      tAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaactt                                                                 <  1:7972‑M2/151‑84 (MQ=255)
                                                                                          aacccgaaGCAAAGCATTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacct                                                             <  1:657668‑M2/148‑88 (MQ=255)
                                                                                            aCCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttc                                                           >  2:426283‑M2/1‑62 (MQ=255)
                                                                                              agaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGc                                                         <  1:170021‑M2/95‑1 (MQ=255)
                                                                                                  gCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgac                                                     <  1:417628‑M2/151‑96 (MQ=255)
                                                                                                   cAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgac                                                     >  2:516710‑M2/1‑55 (MQ=255)
                                                                                                    gaactgactgagtcagccgagaagaatttcccagattattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTCAAACCGAACAGCACTACGCCGTCGATATTACGCCGATTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGa                                                    >  1:781827‑M2/51‑150 (MQ=255)
                                                                                                     aaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGaaa                                                  <  2:454243‑M2/102‑1 (MQ=255)
                                                                                                      aGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtg                                                 <  1:253551‑M2/151‑100 (MQ=255)
                                                                                                       atgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAAc                                                >  2:101155‑M2/48‑151 (MQ=255)
                                                                                                        ccTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaa                                               >  2:262431‑M2/1‑50 (MQ=255)
                                                                                                            ttGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattc                                            >  2:716889‑M2/1‑46 (MQ=255)
                                                                                                                gTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagca                                        >  1:257505‑M2/1‑42 (MQ=255)
                                                                                                                 cagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTcc                                       >  1:191632‑M2/38‑150 (MQ=255)
                                                                                                                 cagccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTcc                                       >  1:295742‑M2/38‑150 (MQ=255)
                                                                                                                   gccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGAGATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCcatc                                    <  1:673853‑M2/116‑1 (MQ=255)
                                                                                                                    cgcgCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagta                                     <  2:403761‑M2/149‑112 (MQ=255)
                                                                                                                    ccgagaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCcatc                                    >  2:739310‑M2/35‑150 (MQ=255)
                                                                                                                     gcgcCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggcgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaag                                   <  2:162017‑M2/150‑114 (MQ=255)
                                                                                                                      cgcCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcg                                 >  1:235417‑M2/1‑36 (MQ=255)
                                                                                                                       gcCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgc                                <  2:551138‑M2/151‑117 (MQ=255)
                                                                                                                       gcCAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgc                                <  1:603376‑M2/151‑117 (MQ=255)
                                                                                                                       agaagaatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATg                                 >  2:40809‑M2/32‑150 (MQ=255)
                                                                                                                        ccAGCAGAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgcc                               <  2:586187‑M2/151‑118 (MQ=255)
                                                                                                                         cagcagAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccg                              <  2:733085‑M2/151‑119 (MQ=255)
                                                                                                                          agcagAACCAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgt                             >  2:729354‑M2/1‑32 (MQ=255)
                                                                                                                            aatttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTggg                           >  1:211829‑M2/27‑151 (MQ=255)
                                                                                                                               ttccccgcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTGGGTCg                        >  2:252804‑M2/24‑151 (MQ=255)
                                                                                                                                  cAGCGATGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagacc                       <  2:406288‑M2/149‑126 (MQ=255)
                                                                                                                                     gcttattcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTGGGTCGTAACCt                  >  2:428716‑M2/18‑151 (MQ=255)
                                                                                                                                        tGACTGCCAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaa                  <  1:17619‑M2/148‑131 (MQ=255)
                                                                                                                                         attcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTGGGTCGTAACCTTGtt              >  2:564360‑M2/14‑151 (MQ=255)
                                                                                                                                         attcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTGGGTCGTAACCTTGtt              >  2:287974‑M2/14‑151 (MQ=255)
                                                                                                                                          ttcgcaccttccCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTGGGTCGTAACCTTGTTc             >  1:560727‑M2/13‑151 (MQ=255)
                                                                                                                                              ccAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagatttt         <  1:271971‑M2/151‑140 (MQ=255)
                                                                                                                                              ccAGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagatttt         >  2:419823‑M2/1‑12 (MQ=255)
                                                                                                                                                aGTGGGCTAAggaaggtgcgaacaagtccctgatatgagatcgtgtttggcatctggagcgatagaacagggttcatcatgagtcatcaacttaccgacgccgacagtgaagtcagcagtaagctccgtcagaccagaaaagagatta          >  1:19257‑M2/1‑10 (MQ=255)
                                                                                                                                                   tccCTACCATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTGGGTCGTAACCTTGTTCATAGAACGc    >  1:478049‑M2/4‑151 (MQ=255)
                                                                                                                                                     gCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcc  >  2:603442‑M2/1‑5 (MQ=255)
                                                                                                                                                     cCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTGGGTCGTAACCTTGTTCATAGAACGCTg  <  2:418063‑M2/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACATTTCTTCTGTTATGCCAGTAAAACCGAACAGCACTACGCCGTCGATATTACGCCGTTTCAGCACTCCCAAATGTTCGGCAACTAATTGCGGGGAAAACTGACTTTCCATCATGATTGGGTCGTAACCTTGTTCATAGAACGCTG  >  NC_000913/4466691‑4466990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.