Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,443	0	A	C	47.9%	63.6 / 17.0	48	Y40S (TAC→TCC)	folX	D‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase
Reads supporting (aligned to +/- strand):  ref base A (24/1);  new base C (0/23);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.55e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTG  >  NC_000913/2421296‑2421589                                                                                                                                                     |                                                                                                                                                   aTCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTAccc                                                                                                                                                 <  2:836103/151‑1 (MQ=255)       aGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTAACGATCCACTACCccgcc                                                                                                                                             >  1:495404/1‑149 (MQ=255)         tAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCccgccg                                                                                                                                            >  2:617534/1‑148 (MQ=255)          aaaaCAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATa                                                                                                                                         >  2:432144/1‑150 (MQ=255)               aacGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAgcgc                                                                                                                                   >  1:27644/1‑151 (MQ=255)               aacGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAgcgc                                                                                                                                   >  2:84594/1‑151 (MQ=255)                  ggAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAgcgcgc                                                                                                                                 >  2:819181/1‑150 (MQ=255)                       aCCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGc                                                                                                                           >  1:637153/1‑151 (MQ=255)                          tGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGa                                                                                                                         >  1:727486/1‑150 (MQ=255)                              aTGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGat                                                                                                                     >  2:236153/1‑150 (MQ=255)                                      cccctctattattatTCTAAAAAAGCCCCTTCTTTTGCTTACTTTTACCGAAATAAAGAAAAAAAAAATAAACACCCGTAAGGATT‑TTTTTAACAATGTGCCCCCCCCCCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAAc                                                                                                               <  1:517937/139‑1 (MQ=255)                                      aCCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGAt                                                                                                            >  2:95659/1‑151 (MQ=255)                                           ccGCTATTATTCGTAAAAAGACCCTTCTTTTGCGTCCTTTTACGAAAATAAAGAAAGAAAAATTAAACACCCGTCAGTATT‑TTTTTAAAAATGTAACGTCCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAAc                                                                                                               <  2:375772/143‑1 (MQ=255)                                               tattatTGGAATAAAAAACCTCCGTTTGTTTTCTTTAAGAAAAAAAAAGGAA‑AAAAAATTAAACACCGTGAAGGTATTTGTTACAAATGTGCCGACCCTCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATACCAACGa                                                                                                             <  1:51820/141‑1 (MQ=255)                                                    ttctaaaaaaaacccTTCTTTTGCGTTCGTTTATCGAAATTAAGAAAGAAAAAATAACCACCCGTCAGGATA‑TTGTTATAAAGGGGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATc                                                                                               <  2:611329/137‑1 (MQ=255)                                                    ttCGTAAAAAGACCCTTTTTTGGCGTACTTTTATGGAAATAAAGAAAAAAAAAATAAACACCCGTCAGAATT‑TTTTTATAAATGTGACGATCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATc                                                                                               <  2:378050/150‑1 (MQ=255)                                                    ttCGTAAAAAGACCCTTCGTTGGGGTGTTTTTATCGAAATTAGGAAAAAAAAAATAACCACCCGTAAGAATT‑TTTTTATAATTGTGTCGACCCACTCCTCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTa                                                                                                 <  2:380592/148‑1 (MQ=255)                                                             aGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAAc                                                                                     >  1:312027/1‑151 (MQ=255)                                                                   ttCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCTGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaaa                                                                                >  1:695834/1‑150 (MQ=255)                                                                    tCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAAc                                                                              >  2:547380/1‑151 (MQ=255)                                                                             tccttttATCGAAATTAAGAAAAAAAAAATTAACACCCGTCAGAATA‑TTGTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCa                                                                      <  2:806998/146‑1 (MQ=255)                                                                              aCGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGc                                                                    >  2:431100/1‑151 (MQ=255)                                                                                 tttATCGAAATAAAGGAAAAAAAAATAAACACCCGTAAGGATT‑TTTTTATAAAGGTGACGACCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTc                                                                       <  1:237542/145‑1 (MQ=255)                                                                                 tttATCGAAATAAAGAAAGAAAAAATAAACAACGTCCAGGATT‑TTGTTAACAATGGCACTACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCa                                                                      <  2:429362/146‑1 (MQ=255)                                                                                  ttATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGt                                                                >  1:744334/1‑151 (MQ=255)                                                                                  ttAGCGAAATAAAGGAAAAAAAAATAAACACCCGTCAGAATA‑TTTTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATg                                                                 <  2:495718/150‑1 (MQ=255)                                                                                    aTCGAAATAAAGGAAAAAAAAATTAACACCCGTCAGGATT‑TTGTTATCAATGTGACGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTag                                                              <  2:392051/151‑1 (MQ=255)                                                                                     tggaaaTAAAGGAAAAAAAAATTAAAACCCGTGAGTATT‑TTTTTATAAATGTGACTTTCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTag                                                              <  1:188627/146‑1 (MQ=255)                                                                                        gAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAgaga                                                           >  1:375501/1‑150 (MQ=255)                                                                                         aaTAAAGGAAAAAAAAATAAACACCCAACAGGATT‑TTGTTATAAATGTGCCCCCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGaa                                                          <  2:684531/150‑1 (MQ=255)                                                                                                    aagaaATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTtctc                                              >  2:694409/1‑151 (MQ=255)                                                                                                    aaaaaaTAAACACCCGTCAGGATT‑TTTTTATAAATGTGACGCCCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTtctc                                              <  1:763093/148‑1 (MQ=255)                                                                                                       aaaTTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCttt                                           >  2:483619/1‑151 (MQ=255)                                                                                                       aaaTAAACACCCGAAAGTATT‑TTGTTATCATTGTGACTACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCttt                                           <  2:413855/151‑1 (MQ=255)                                                                                                               acccataaGGATT‑TTTTTACCAAGGTAACCACCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTg                                       <  1:464088/140‑1 (MQ=255)                                                                                                               acccGTAAGAATT‑TTTTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGa                                     <  2:785172/147‑1 (MQ=255)                                                                                                                  cGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAAt                                >  1:577101/1‑151 (MQ=255)                                                                                                                      aGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAAc                            >  1:731559/1‑151 (MQ=255)                                                                                                                           tttttttATCAATGTGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACt                           <  2:299185/143‑1 (MQ=255)                                                                                                                              ttttAACAATGTGACGCCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGa                      <  2:577165/148‑1 (MQ=255)                                                                                                                                ttATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGAtg                    >  2:578779/1‑150 (MQ=255)                                                                                                                                 taccAATGTGCCGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGAtgt                   <  2:509108/147‑1 (MQ=255)                                                                                                                                    cAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCt                >  2:517919/1‑150 (MQ=255)                                                                                                                                     aaTGGGACGACCAACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCt                <  2:835932/149‑1 (MQ=255)                                                                                                                                          taCGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGatat          <  1:361766/149‑1 (MQ=255)                                                                                                                                              atacACTACCCCGCAGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTAAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCa      >  1:204319/4‑150 (MQ=255)                                                                                                                                              aTCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCAc     >  2:484396/1‑151 (MQ=255)                                                                                                                                                 cACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCAAGTg  <  2:874065/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATA‑TTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTG  >  NC_000913/2421296‑2421589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑