Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	50.9%	54.0 / 83.8	53	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (4/22);  new base G (27/0);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 3.23e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-08

GGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGG  >  NC_000913/354142‑354426                                                                                                                                                   |                                                                                                                                            gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                         <  1:458485/151‑1 (MQ=255)   gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                       <  1:353963/151‑1 (MQ=255)       ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTTGGTGGGGGGGAAg                                                                                                                                        >  1:186969/1‑146 (MQ=255)         gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGaaa                                                                                                                                         >  2:162222/1‑143 (MQ=255)           gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCt                                                                                                                                 >  2:549414/1‑149 (MQ=255)           gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGGAAGATggg                                                                                                                                   >  1:373717/1‑145 (MQ=255)                   cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGGAGCGCTggctcag                                                                                                                          >  1:480275/1‑142 (MQ=255)                   cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGAGCTGgaaag                                                                                                                           >  2:447249/1‑145 (MQ=255)                     gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                                                                     <  2:821960/151‑1 (MQ=255)                      ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGGAAGGGGAgggg                                                                                                                    >  2:414474/1‑149 (MQ=255)                           aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCAGCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt‑ggcggt                                                                                                              <  1:744500/151‑1 (MQ=255)                             tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGGCGGAAGGGGAggg‑gggggggg                                                                                                            >  1:389387/1‑151 (MQ=255)                              ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGGTGCCGAAGTGGCGGTGGGGGGGGGGTAAGATGCGCTGAAAGGGCAggt‑gggggcgg                                                                                                            >  1:570274/1‑150 (MQ=255)                                   ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGG‑GGGGGGGGCGttt                                                                                                       >  2:371864/1‑150 (MQ=255)                                      gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGGGCTGAAAGGGGAggg‑gggggggggggtt                                                                                                       >  2:405918/1‑142 (MQ=255)                                          gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTcc                                                                                               <  2:819426/151‑1 (MQ=255)                                             cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAGATGCGCTGAAAAGGCAGGG‑GGGGGGGGGGGTTGGCATTTCg                                                                                              >  1:841709/1‑149 (MQ=255)                                               gACGATGTAATTAACGTTGCCGGTCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAag                                                                                          <  1:560945/151‑1 (MQ=255)                                                  gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAagaga                                                                                       <  2:790895/151‑1 (MQ=255)                                                  gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGG‑GGGGGGGGCGGTTGTGTTTCCGgaa                                                                                           >  1:814796/1‑147 (MQ=255)                                                     gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGCGGG‑GGGGGGGGGGGTTTTCATTTCGgaa                                                                                           >  1:573114/1‑144 (MQ=255)                                                       aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                                                                                  <  2:376097/151‑1 (MQ=255)                                                        aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                 <  1:333155/151‑1 (MQ=255)                                                           aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAAGGCTGGAAGGGCGGGTGGGGGGTGGGGTTTGTCTTTCTGaagaaagcgga                                                                                   >  1:724742/1‑138 (MQ=255)                                                              gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                           <  2:154013/151‑1 (MQ=255)                                                              gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAGTGCGCCGGAAGGGGAGGG‑GGGGGGGGCGGTTGTCCTTCCGAAAGAGAGGGGCAGGCTgg                                                                           >  1:14423/1‑151 (MQ=255)                                                                tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGAAAAGGCGGGG‑GGCGGGGGGGGTTGTCCTTTCGCAAGAGAGCGGCAggc                                                                              >  1:169561/1‑144 (MQ=255)                                                                  ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCCGAAAAGGCAGGG‑GGGGGGGGGGGTTggcctttccgaa                                                                                           >  1:692859/1‑120 (MQ=255)                                                                   cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGG‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                           >  2:425776/1‑146 (MQ=255)                                                                    gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                     <  1:137763/151‑1 (MQ=255)                                                                      gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGCGGG‑GGGGGGGGGGGTTGTCATTCCGGAAGAGAGCGGAAGTCTGGgaga                                                                       >  2:481874/1‑147 (MQ=255)                                                                       cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTg                                                                  <  2:353861/151‑1 (MQ=255)                                                                        aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCTGAAAGGGCAGGG‑GGGGGGGGGGTTTGTCATTCCGAAAAAGAGCGACAGTCTGGGAGATCg                                                                    >  2:674025/1‑148 (MQ=255)                                                                          cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAGGCGCTGGAAAGGCAGGG‑GGGGGTGGGGTGTGTGACTCCGAGAGAGAGCGACAGTCTGGGAGAACggg                                                                  >  1:359734/1‑146 (MQ=255)                                                                           ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt                                                              <  1:616377/151‑1 (MQ=255)                                                                           ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGG‑GGGGGGGGGGGTTGTCATTTCGAAAAAGAGGGACAGTCTGGAAGAACGGGAtgt                                                              >  2:599788/1‑151 (MQ=255)                                                                             cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAGATGCGCTGAAAGGGGAGGG‑GGGGGGGGGGGTTGTTATTCCGAAAGAGAGCGACAGGCTGGAAGATTGTGa                                                                 >  2:749243/1‑146 (MQ=255)                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                          <  2:814892/151‑1 (MQ=255)                                                                                         gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGCGGG‑GGCGGGGGCGTTTGTCCTTCCGAAAGAGAGCGGCAGGCTTGAAGAGCGGGATGTGGCGCACTc                                                     >  1:803663/1‑146 (MQ=255)                                                                                          agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAAATGCGCTGGAAGGGGAGGG‑GGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGGGCACTCGCa                                                  >  1:309440/1‑148 (MQ=255)                                                                                                    gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGACAGATGCGCTGAAAGGGCCGGT‑AGCGGTGGCGTTTGTCATTCCGAAAGCGAGCGACAGTCTGGACGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAta                                     <  1:583066/151‑2 (MQ=255)                                                                                                    gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCCGGT‑GGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                     >  2:762331/1‑151 (MQ=255)                                                                                                      gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTa                                    <  1:848593/150‑1 (MQ=255)                                                                                                         tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGAATCGCCGAAAGGGCAGGG‑GGCGGGGGGGGTTGGCCTTCCCGAAGAGGGCGGCAGTCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGCTTATgg                                 >  1:405221/1‑150 (MQ=255)                                                                                                            ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgc                                <  2:515654/148‑1 (MQ=255)                                                                                                             cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg                            <  1:312441/151‑1 (MQ=255)                                                                                                                  aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgc                                <  1:668345/142‑1 (MQ=255)                                                                                                                  aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGGGGGGGTTGTCCTTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                        >  2:489852/1‑150 (MQ=255)                                                                                                                     cGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAg                    <  2:602339/151‑1 (MQ=255)                                                                                                                      gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGc                   <  2:785074/151‑1 (MQ=255)                                                                                                                              ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg           <  1:202452/151‑1 (MQ=255)                                                                                                                                 aaGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGGGGCGGTTGTCATTCCGAAAAAGAGCGACAGTCTGGGAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCa         >  1:85209/1‑150 (MQ=255)                                                                                                                                       cGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGAGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGCTTATGGCGCTGGGGGACAGCCAGATTGGCAACTTTgg  >  2:600127/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                            GGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGT‑GGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGG  >  NC_000913/354142‑354426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑