Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,369,969	T→A	T128S (ACC→TCC)	nanK ←	N‑acetylmannosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,369,969	0	T	A	100.0%	200.9 / NA	52	T128S (ACC→TCC)	nanK	N‑acetylmannosamine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (16/36);  total (16/36)

CCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATT  >  NC_000913/3369828‑3370116                                                                                                                                              |                                                                                                                                                    ccACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGAc                                                                                                                                            >  1:316880/1‑151 (MQ=255) ccACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACCGAGATAAAGAc                                                                                                                                            >  2:86861/1‑151 (MQ=255) ccACACAACCTGTGCGTCCACGGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGGATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAg                                                                                                                                              <  1:852862/149‑1 (MQ=255)      cacccTGTGCGTCCACACCCGCGGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCatat                                                                                                                                       <  1:722096/148‑1 (MQ=255)              gCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTta                                                                                                                                 <  1:135832/149‑1 (MQ=255)                 tCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATc                                                                                                                             <  2:84630/150‑1 (MQ=255)                            aGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAgcg                                                                                                                  <  2:684436/150‑1 (MQ=255)                                tGGGCGGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCt                                                                                                               <  2:283114/149‑1 (MQ=255)                                     cGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTc                                                                                                        >  1:575048/1‑151 (MQ=255)                                         tgGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGcc                                                                                                    >  1:342213/1‑151 (MQ=255)                                           gATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGccc                                                                                                   >  1:704626/1‑150 (MQ=255)                                            aTCGGCATGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAATCAGTTTGCAGCCGATCTCTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGTTTATATCGCCATCCAGCGCCTGAAACTCCGCCCAt                                                                                                 <  2:748945/151‑1 (MQ=255)                                                  aaGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCg                                                                                           >  2:783595/1‑151 (MQ=255)                                                               atatGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGcgtcgt                                                                              <  2:89294/151‑1 (MQ=255)                                                               atatGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGcgtcgt                                                                              <  1:228933/151‑1 (MQ=255)                                                                 atGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTa                                                                            <  2:342250/151‑1 (MQ=255)                                                                   gCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTaa                                                                           <  1:404764/150‑1 (MQ=255)                                                                   gCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAAt                                                                          <  2:596935/151‑1 (MQ=255)                                                                    ccccGCCAGACCGCCATGGCCGGTAAGCCGTTTGCAGCCGCTCACTCCACCGCCGCCAACGCCGGTGGAAACGGAGTTAAAGACCATATCGATTATATCGCCCTCCAGCGCCTGAAACTCCGCCCATGCTGCTGCTTGCGCGTCGTTaagg                                                                         >  1:579353/1‑149 (MQ=255)                                                                        gCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACGCCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCa                                                                      <  1:42670/150‑1 (MQ=255)                                                                        gCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCa                                                                      >  1:292640/1‑150 (MQ=255)                                                                              ccGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCg                                                               <  2:853687/151‑1 (MQ=255)                                                                              ccGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCg                                                               <  1:81860/151‑1 (MQ=255)                                                                                 ccAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGc                                                             <  1:260182/150‑1 (MQ=255)                                                                                 ccAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGc                                                             <  1:814127/150‑1 (MQ=255)                                                                                 ccAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGc                                                             >  1:483744/1‑150 (MQ=255)                                                                                     ggCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAAtt                                                        <  1:267610/151‑1 (MQ=255)                                                                                      gCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTg                                                       <  1:339425/151‑1 (MQ=255)                                                                                       ccGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTgg                                                      <  2:793518/151‑1 (MQ=255)                                                                                          gTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTaa                                                   <  2:795150/151‑1 (MQ=255)                                                                                          gTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTaa                                                   <  2:508131/151‑1 (MQ=255)                                                                                            aaGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgt                                                 <  2:306266/151‑1 (MQ=255)                                                                                              gCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttg                                               <  1:57605/151‑1 (MQ=255)                                                                                              gCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttg                                               <  2:521766/151‑1 (MQ=255)                                                                                               cAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttgt                                              <  2:446190/151‑1 (MQ=255)                                                                                                     gCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGc                                        <  2:292677/151‑1 (MQ=255)                                                                                                      cAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCg                                       >  2:400381/1‑151 (MQ=255)                                                                                                      cAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCg                                       >  2:790890/1‑151 (MQ=255)                                                                                                        gCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACGCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGtt                                     <  1:254447/151‑1 (MQ=255)                                                                                                          cGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGtttt                                   <  1:809132/151‑1 (MQ=255)                                                                                                             tCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGgttt                                   >  1:158572/1‑148 (MQ=255)                                                                                                                  acacCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAAc                            <  2:295684/150‑1 (MQ=255)                                                                                                                       gccgccAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAg                      <  2:93395/151‑1 (MQ=255)                                                                                                                        ccgccAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAgt                     <  1:44190/151‑1 (MQ=255)                                                                                                                             aaCGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGTAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGc                <  1:47851/151‑1 (MQ=255)                                                                                                                                gCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAAt             >  1:249392/1‑151 (MQ=255)                                                                                                                                gCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAAt             >  1:143751/1‑151 (MQ=255)                                                                                                                                   ggTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATcca          <  1:669897/151‑1 (MQ=255)                                                                                                                                     tGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATccacc        >  2:676196/1‑151 (MQ=255)                                                                                                                                           cGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGAtt  >  2:641196/1‑151 (MQ=255)                                                                                                                                           cGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGAtt  <  2:812476/151‑1 (MQ=255)                                                                                                                                           cGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGAtt  <  1:148457/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    CCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATT  >  NC_000913/3369828‑3370116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑