Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,931,668	T→G	L263W (TTG→TGG)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,931,668	0	T	G	100.0%	238.3 / NA	63	L263W (TTG→TGG)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/32);  total (31/32)

ATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGG  >  NC_000913/1931527‑1931814                                                                                                                                              |                                                                                                                                                   aTTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGgtgt                                                                                                                                           <  1:374250/151‑1 (MQ=255)       gtgCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGa                                                                                                                                        <  1:350303/148‑1 (MQ=255)          cACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTAtt                                                                                                                                  >  1:854118/1‑151 (MQ=255)            ccAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTAttt                                                                                                                                 <  1:268315/150‑1 (MQ=255)            ccAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTg                                                                                                                                >  2:117187/1‑151 (MQ=255)              aGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAAAGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGATATGGGTGGTTTGGTGTCGAGCTATTTGTc                                                                                                                              >  1:273947/1‑151 (MQ=255)               gTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCt                                                                                                                             >  2:410970/1‑151 (MQ=255)                    tCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTgtgg                                                                                                                         >  1:650381/1‑150 (MQ=255)                        cGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGtgat                                                                                                                     <  2:596420/150‑1 (MQ=255)                          ccAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTAGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGGGTCGAGCTATTTGTCTGTGGtga                                                                                                                      >  1:773318/1‑147 (MQ=255)                           cAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAgg                                                                                                                 >  2:53038/1‑151 (MQ=255)                           cAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAgg                                                                                                                 <  2:864555/151‑1 (MQ=255)                           cAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAgg                                                                                                                 <  1:759621/151‑1 (MQ=255)                           cAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAgg                                                                                                                 <  2:784522/151‑1 (MQ=255)                            aGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAgg                                                                                                                 >  1:402906/1‑150 (MQ=255)                               aaGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTg                                                                                                               >  2:283152/1‑149 (MQ=255)                                 gATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGAttt                                                                                                           <  1:541727/151‑1 (MQ=255)                                  aTGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGAtttt                                                                                                          >  1:678277/1‑151 (MQ=255)                                  aTGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGAtttt                                                                                                          <  2:833980/151‑1 (MQ=255)                                     gCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTc                                                                                                         >  1:656404/1‑149 (MQ=255)                                                    ccTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTc                                                                                         <  2:650069/150‑1 (MQ=255)                                                      tGGCACCCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTc                                                                                      >  1:466104/1‑151 (MQ=255)                                                        gCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCca                                                                                    <  2:718361/151‑1 (MQ=255)                                                         cAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCcac                                                                                   <  2:563905/151‑1 (MQ=255)                                                         cAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCCGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCaac                                                                                   <  1:93483/151‑1 (MQ=255)                                                            ccACAGCAAATGAGCCCGCTTGCGCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTTTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCGCAt                                                                                 <  1:444948/150‑1 (MQ=255)                                                                atcAAATGAGCCGGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATAc                                                                            >  1:36185/3‑151 (MQ=255)                                                                  cAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCg                                                                          >  1:444581/1‑151 (MQ=255)                                                                   aaaTGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCgg                                                                         >  2:256997/1‑151 (MQ=255)                                                                       gAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGAt                                                                      <  1:611357/150‑1 (MQ=255)                                                                       gAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGCGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATg                                                                     >  1:520635/1‑151 (MQ=255)                                                                           ccGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTg                                                                  >  2:421575/1‑150 (MQ=255)                                                                             gCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACg                                                               <  2:783456/151‑1 (MQ=255)                                                                             gCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACg                                                               >  2:865893/1‑151 (MQ=255)                                                                                 gCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTa                                                            <  2:346123/150‑1 (MQ=255)                                                                                   ccTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGCGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGCTAAt                                                          >  1:418220/1‑150 (MQ=255)                                                                                        aaCGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTtctc                                                     >  2:267609/1‑150 (MQ=255)                                                                                        aaCGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTtctc                                                     >  1:227139/1‑150 (MQ=255)                                                                                        aaCGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCa                                                    <  1:53477/151‑1 (MQ=255)                                                                                           gTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGa                                                 <  2:147956/151‑1 (MQ=255)                                                                                            tGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCa                                                    >  1:301137/1‑147 (MQ=255)                                                                                             gcgcAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGAtc                                               <  2:354929/151‑1 (MQ=255)                                                                                                 aGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGAtctctc                                           >  2:673650/1‑151 (MQ=255)                                                                                                  ggAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCa                                          <  1:164470/151‑1 (MQ=255)                                                                                                   gagaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCag                                         <  1:528152/151‑1 (MQ=255)                                                                                                    agaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGAtctctc                                           >  2:423420/1‑148 (MQ=255)                                                                                                    agaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCaga                                        <  1:633322/151‑1 (MQ=255)                                                                                                    agaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCaga                                        <  2:795339/151‑1 (MQ=255)                                                                                                      aTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGt                                      <  1:584358/151‑1 (MQ=255)                                                                                                         gCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTg                                   <  1:636750/151‑1 (MQ=255)                                                                                                           ccGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGcc                                 <  2:30172/151‑1 (MQ=255)                                                                                                           ccGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGcc                                 >  1:480078/1‑151 (MQ=255)                                                                                                               aaaGTGGTGCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGc                             >  1:294257/1‑151 (MQ=255)                                                                                                                aaGTGGTGCTGGCACTGGGCGGTTATGGGAGGGTTGGTGTCGAGATATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGAGCACATGGTACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGc                             >  1:710996/1‑150 (MQ=255)                                                                                                                       gCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTAc                       <  1:452231/149‑1 (MQ=255)                                                                                                                       gCTGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGt                     >  1:13175/1‑151 (MQ=255)                                                                                                                         tGGCACTGGGCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGc                   >  2:8470/1‑151 (MQ=255)                                                                                                                                 ggCGGTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCg           <  1:602969/151‑1 (MQ=255)                                                                                                                                    ggTTATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGAc        <  2:280680/151‑1 (MQ=255)                                                                                                                                      ttATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACtt      >  1:844470/1‑151 (MQ=255)                                                                                                                                       tATGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACtt      <  1:521951/150‑1 (MQ=255)                                                                                                                                        aTGGGTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTcc    >  1:641758/1‑151 (MQ=255)                                                                                                                                          gggTGGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCgg  <  2:551426/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                   ATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCCTGGCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGG  >  NC_000913/1931527‑1931814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑