Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,128	T→G	L5R (CTC→CGC)	tolC →	transport channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,128	0	T	G	100.0%	193.5 / NA	52	L5R (CTC→CGC)	tolC	transport channel
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/25);  total (27/25)

CATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAA  >  NC_000913/3177981‑3178270                                                                                                                                                    |                                                                                                                                               cATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGccc                                                                                                                                             >  2:149955/1‑151 (MQ=255)        tAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCt                                                                                                                                       <  1:563923/150‑1 (MQ=255)         aaaTTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCtt                                                                                                                                      >  2:18035/1‑150 (MQ=255)          aaTTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTAt                                                                                                                                    <  1:289734/151‑1 (MQ=255)             tGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCg                                                                                                                                  <  1:427009/150‑1 (MQ=255)               aGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGcc                                                                                                                               <  2:794260/151‑1 (MQ=255)                 gCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTg                                                                                                                             <  1:626451/151‑1 (MQ=255)                  cacaTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGa                                                                                                                            <  2:705379/151‑1 (MQ=255)                  cacaTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGa                                                                                                                            <  2:301063/151‑1 (MQ=255)                   acaTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAg                                                                                                                           <  1:352881/151‑1 (MQ=255)                    caTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGc                                                                                                                          >  2:673229/1‑151 (MQ=255)                      ttAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCt                                                                                                                        >  1:548988/1‑151 (MQ=255)                          cGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCt                                                                                                                    <  1:49340/151‑1 (MQ=255)                            cccTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTgg                                                                                                                  >  1:502347/1‑151 (MQ=255)                                 tGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGtt                                                                                                               >  1:216390/1‑149 (MQ=255)                                   gCACGTAACGCCAACCTTTTG‑GGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGtt                                                                                                          >  2:532650/1‑151 (MQ=255)                                     aCGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTc                                                                                                         <  2:279066/151‑1 (MQ=255)                                         aaCGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGtt                                                                                                      <  2:534126/150‑1 (MQ=255)                                          aCGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGa                                                                                                    <  1:109942/151‑1 (MQ=255)                                                       gCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCgaga                                                                                        <  1:765921/150‑1 (MQ=255)                                                       gCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCgag                                                                                         >  2:805832/1‑149 (MQ=255)                                                         ggTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAAcc                                                                                     >  1:34439/1‑151 (MQ=255)                                                          gTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCt                                                                                    <  1:160110/151‑1 (MQ=255)                                                            aGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTg                                                                                   >  2:364246/1‑150 (MQ=255)                                                               ggCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGc                                                                               >  2:421912/1‑151 (MQ=255)                                                                       cTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTa                                                                        >  2:197520/1‑150 (MQ=255)                                                                          gAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTa                                                                        >  2:310516/1‑147 (MQ=255)                                                                                 cAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCAc                                                             <  2:48924/151‑1 (MQ=255)                                                                                     aatTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGcc                                                          <  2:727764/150‑1 (MQ=255)                                                                                        tttACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCtt                                                        >  1:599455/1‑149 (MQ=255)                                                                                          tACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAg                                                      <  2:578357/149‑1 (MQ=255)                                                                                          tACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGt                                                     >  2:372452/1‑150 (MQ=255)                                                                                           aCAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTa                                                    <  1:177947/150‑1 (MQ=255)                                                                                              gTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAAccc                                                >  2:753329/1‑151 (MQ=255)                                                                                              gTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAAccc                                                >  1:838584/1‑151 (MQ=255)                                                                                               tttGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCg                                               >  2:650208/1‑151 (MQ=255)                                                                                                     cgcgCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTg                                         <  2:800317/151‑1 (MQ=255)                                                                                                         cTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGt                                      >  1:753410/1‑150 (MQ=255)                                                                                                            aaTACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGt                                  >  1:343287/1‑151 (MQ=255)                                                                                                                  gCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTgcc                             <  2:746892/150‑1 (MQ=255)                                                                                                                    ttCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTgccgcc                          >  1:162426/1‑151 (MQ=255)                                                                                                                      caccacAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGccgccg                         >  1:127511/1‑150 (MQ=255)                                                                                                                             aGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGAt                 >  2:228303/1‑151 (MQ=255)                                                                                                                                      aaTGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCttt        <  2:281431/151‑1 (MQ=255)                                                                                                                                      aaTGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAATTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTGGCAGCAAGCACGCCTTAGTAGCCCGGAATTGCGGAAGTCTGCCGCCGATCGTGATGCTGCCttt        <  1:649854/151‑1 (MQ=255)                                                                                                                                         gaagaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGt                                      <  1:862203/118‑1 (MQ=255)                                                                                                                                         gaagaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGt                                      >  2:862302/1‑118 (MQ=255)                                                                                                                                           agaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCTGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaaa   <  1:685490/151‑1 (MQ=255)                                                                                                                                           agaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaaa   <  2:37712/151‑1 (MQ=255)                                                                                                                                           agaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaa    >  2:491147/1‑150 (MQ=255)                                                                                                                                            gaaATTGCGCGCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTAGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTCGTAACCCGGACTTGCGAAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaaa   >  1:353661/1‑150 (MQ=255)                                                                                                                                             aaaTTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaaaa  >  2:353911/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                               CATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAA  >  NC_000913/3177981‑3178270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑