| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,576,077 | 0 | C | A | 45.8% | 22.3 / 14.9 | 24 | D515Y (GAT→TAT) | yddB | putative TonB‑dependent outer membrane receptor |
| Reads supporting (aligned to +/- strand): ref base C (9/4); new base A (0/11); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
TCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGC > NC_000913/1575942‑1576219 | tCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGc < 1:646605/150‑1 (MQ=255) ctgcAACCCCATTGCCATTTCATCTTTATAAGGTTTTTTAAAATCCTAAAAACGCGTCATTTTTTTATTCCCTAACCCCAATTCCCCCCAGCTATTGCGAATTTCACGTAACCCCATAACAAGAATATTCCCGCCATAGTAACGGtt < 1:149859/147‑1 (MQ=255) cAATCCCATTGCCAGTTCATCTTAATAAGGCTTTTCCAAACCCTGATATCGCGTTATATTTTTATTCCCTGATCCCGATCCCCTCCATCTATGGCGGATATCCCATAACCCAAAATAAAAAATTTTCCCGCCATAGTAACGGt < 1:715872/143‑1 (MQ=255) ttccaaGTTCTTCTTTAAAAGGTTTTTAAAAATCCTAATACCGCGTCAGATTTTTTTTCCCTAATACCTATTCCGTCCATTTTTTGCGTATATCATATAACCCAATAAAAAAAATATCCCCGCCATAGTAACGGTTATAACCTGCTGTaa < 2:819152/145‑1 (MQ=255) ttGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAAt > 2:630043/1‑151 (MQ=255) gTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATGTCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTg > 1:789724/1‑150 (MQ=255) agggttttttCAAATCTTGAAACCGCGTAAGTTTTTTTTTCCCTAATACCGATTCCGTCCAGTTATTGGGGATATCACTTAACCCAATATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGc < 2:666031/145‑1 (MQ=255) aaggttttttCAAATCCTAAAAACGCGTCAAATTTTTTTTCCCTAATCCCTATCCCTCCCAGCTATTGCGTATATCACATACCCCAATAAAAAAATTTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTa < 1:16820/143‑1 (MQ=255) ttttAAAATCTTAATACCGCTTCAGATTTTTATTCCCGGATCCCGATCCCGCCCAGCTATTGGGAATACCACGTACTCCCAAAAAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaa < 2:292919/149‑1 (MQ=255) aTCCTGATAATGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCa > 1:478542/1‑150 (MQ=255) aTACCGCGTCAGATTTTTTTTCCCTACTACCTATTCCGTCCAGTTATTGAGAATTTCACGTATTCCAATATAAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAata < 1:257926/139‑1 (MQ=255) aaCGCTTCAGATTTTTATTCCCTGATCCCGATCCCCTCCAGCTTTTGCGGATATCACTTACTCCCATAAAAAGAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTc < 1:172889/149‑1 (MQ=255) aaCGCGTAAGATTTTTTTTCCCTAATCCCTATTCCGTCCAGTTATGGCGGATATCACGTAACCCAATATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCa < 1:36616/150‑1 (MQ=255) agagTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGcgcg > 2:463380/1‑151 (MQ=255) ttttATTCCCTGATACCGATCCCGTCCAGCTATTGGGAATATAACAAAACCCCATAAAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCgg < 1:457964/148‑1 (MQ=255) tttATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCgggg > 1:752784/1‑149 (MQ=255) ttatccctatcccGCCCAGCTTTGGGGGATATCACAAAACCCAAAAACAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATAtt < 1:405594/136‑1 (MQ=255) ccGTTTCCGTCCAGCTATGGCGAATATCACGTAACCCAATATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGttt < 2:542165/149‑1 (MQ=255) gTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGt > 1:246593/1‑151 (MQ=255) ccAGTTATTGCGGAATTCACGAACACCAAAAAAAAAAATTTCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAg < 1:62641/148‑1 (MQ=255) cAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTgt > 1:715691/1‑151 (MQ=255) cTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTgtcat > 2:821016/1‑151 (MQ=255) aCGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg > 1:774828/1‑151 (MQ=255) cccATATCAAAAATTTCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGc < 2:434219/150‑1 (MQ=255) | TCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGC > NC_000913/1575942‑1576219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |