Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	41.2%	125.1 / 29.1	80	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (14/33);  new base G (33/0);  total (47/33)
Fisher's exact test for biased strand distribution p-value = 1.10e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434738‑2435032                                                                                                                                                  |                                                                                                                                                           ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCa                                                                                                                                                      <  2:731644/151‑1 (MQ=255) ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCa                                                                                                                                                      <  1:443320/151‑1 (MQ=255)   atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑cc                                                                                                                                                   <  2:113028/151‑1 (MQ=255)    tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGT‑CAGGGGAATCCA‑cc                                                                                                                                                   >  1:29451/1‑150 (MQ=255)     atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGGTAATACCGGG‑CCGGGGAAATCA‑cc                                                                                                                                                   >  2:446179/1‑149 (MQ=255)     atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑Ccg                                                                                                                                                  <  1:561358/150‑1 (MQ=255)       ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTGAATTCTACCGGT‑CCGGGGAATCAC‑Ccgc                                                                                                                                                 >  1:488979/1‑149 (MQ=255)        tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTAAACCCGG‑CAGGGGaaac                                                                                                                                                        >  1:762699/1‑140 (MQ=255)             aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGG‑CAGGGTAATCCA‑CCCCGAcc                                                                                                                                             >  2:361682/1‑147 (MQ=255)                    gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGG‑CAGGGGAATCCA‑CCCCGACCCGAA‑ACAG‑ccc                                                                                                                                >  1:409147/1‑151 (MQ=255)                       cTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTGGGAGATCATAACGGG‑CAGGGGAAAACA‑CCGCGACCAAAA‑AAAG‑CCCGTc                                                                                                                             >  1:678083/1‑151 (MQ=255)                        tCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGT‑CCGGGGAATCCA‑CCCCGACCAGAT‑ACAG‑CCCTTcc                                                                                                                            >  1:352638/1‑151 (MQ=255)                         cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTc                                                                                                                             <  1:768000/149‑1 (MQ=255)                         cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCg                                                                                                                           <  2:96026/151‑1 (MQ=255)                           tCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGGAATTCA‑CCGCGACCAGAT‑ACAG‑CCCTTTCGc                                                                                                                          >  2:695082/1‑150 (MQ=255)                            cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCtt                                                                                                                        <  2:608700/151‑1 (MQ=255)                                 ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCCGG‑CCGGGGAAATCA‑CCCCGCCCCGAT‑ACAG‑CCCCTCCCCttttttc                                                                                                                   >  2:662719/1‑148 (MQ=255)                                 ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCtttt                                                                                                                      <  1:81906/148‑1 (MQ=255)                                 ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCttt                                                                                                                       <  2:87483/147‑1 (MQ=255)                                    aGGCTGTAACGACGTGGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCTGAAATAGCGGGCCCATTGGCGGTTTTGGAAGGTCATACCGGT‑CGGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑AACTTCCGCTTTTGCCGtt                                                                                                                <  1:616399/151‑1 (MQ=255)                                         gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTtgctg                                                                                                            <  1:485943/150‑1 (MQ=255)                                         gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTtgct                                                                                                             <  1:453217/149‑1 (MQ=255)                                         gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CACTTCCGCTTTTGCCGTtgct                                                                                                             <  1:92625/149‑1 (MQ=255)                                         gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGG‑CAGGGGAATCCA‑CCGCGACCAGAA‑ACAG‑CCCTTCCCCTTTTTcc                                                                                                                   >  2:724278/1‑143 (MQ=255)                                          tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGG‑CAGGGGAATCCA‑CCCCGACCCGAT‑ACAG‑CCCCTCCGCCTTTTCCGtt                                                                                                                >  1:737954/1‑145 (MQ=255)                                           aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGATCATACCGGT‑CAGGGGAATACA‑CCGCGACCCGAT‑ACAG‑CCCTTCCGCTTTTTCCGTTTCTgcc                                                                                                          >  1:107949/1‑150 (MQ=255)                                             cTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGG‑CAGGGGAATCCA‑CCGCGACCAGAT‑ACAG‑CCCCTCCCCCTTTTCCCTTTCTgccgcc                                                                                                       >  2:215824/1‑151 (MQ=255)                                                 gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGT‑CAGGGGAATCCC‑CCCCGACCAGAA‑ACAG‑CCCTTCCGCTTTTTCCGTTGCTgccgcc                                                                                                       >  1:758115/1‑147 (MQ=255)                                                   tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAAACCGGT‑CCGGGGAATCCA‑CCGCGACCAGATAAAAG‑CCCTCCCCCTTTT‑TCGTTGCTgccgcc                                                                                                       >  1:206384/1‑145 (MQ=255)                                                   tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGt                                                                                                  <  1:160552/150‑1 (MQ=255)                                                    ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTATACCGGG‑CAGGGGAAACCC‑CCGCGACCCGAT‑ACCG‑CCCCTCCGCCTTTGCCGTTGCTTCCGCCcgcggt                                                                                                 >  1:629076/1‑148 (MQ=255)                                                    ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGG‑GAGGGGAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTTCCGTTGCTGCCCCCcgcg                                                                                                   >  1:159714/1‑148 (MQ=255)                                                    ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATTATACCCGT‑CCGGGGAATCCA‑CCGCGACCCGAT‑ACAA‑CCCTTCCGCTTTTTCCGTTGCTGCCCCCAGCGt                                                                                                  >  2:229872/1‑149 (MQ=255)                                                     gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCt                                                                                               <  2:136618/151‑1 (MQ=255)                                                      cTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCCGT‑CAGGGGAATCCA‑CCCCGACCCGAT‑ACAA‑CCCCTCCGCTTTTTCCGGTGCTTCCGCCcgcggt                                                                                                 >  2:301606/1‑146 (MQ=255)                                                           gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCtt                                                                                        <  2:240887/151‑1 (MQ=255)                                                            ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCttt                                                                                       <  2:110064/151‑1 (MQ=255)                                                            ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCAATTGGCGGTTTTGGAAGATCATACTGGT‑CAGGGTAATCCA‑GCGCGACCAGAT‑ACAG‑CCCTTCCTCAGTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCttt                                                                                       <  1:543473/151‑1 (MQ=255)                                                                aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTgccg                                                                                   <  1:199532/151‑1 (MQ=255)                                                                  aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGtt                                                                                                                <  2:737258/121‑1 (MQ=255)                                                                  aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTgccgcc                                                                                 <  2:82813/151‑1 (MQ=255)                                                                  aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGATTTTGAAGGTCATTCCCGG‑CAGGGGAATCCC‑CCCCGACCCGAC‑ACAGCCCCTTCCG‑TTTTGCCGGTGGTGCCGCCCGCGTTCTGT‑CCTTTgccgcc                                                                                 >  2:872057/1‑151 (MQ=255)                                                                   tGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATAAAAACCGT‑CCGGGGGAACCA‑CCGCGCACACAT‑ACAG‑CGCCTTCGCTTTTTGCGTTGCCTGCGCCCGCGTTCTTT‑TCTTTtcccgc                                                                                 >  1:775350/1‑147 (MQ=255)                                                                    gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCa                                                                                <  2:362413/150‑1 (MQ=255)                                                                    gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCa                                                                                <  2:505180/150‑1 (MQ=255)                                                                      tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGT‑CAGGGGAATCCA‑CCCCGACCCGAT‑ACAA‑CCCCTCCGCTTTTTCCGGTGCTGCCGCCCGCGGTCTGT‑CCTTTGccgcccgc                                                                              >  1:850570/1‑150 (MQ=255)                                                                       ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGG‑CAGGGGAATCCA‑CCCCGGCCAGAT‑ACAG‑CCCCTCCGCTTTTTCCGTTGCTGCCCCCCGCGGTGTGT‑CCtttttcccc                                                                                 >  1:111810/1‑140 (MQ=255)                                                                         gAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATAATAACGGT‑CAGGGGAATCCA‑CCGCGACAAGAT‑ACAG‑CCCTTTCGCGTTTGTCGGTGGTGCCGCCAGCGGTCTGT‑CCTTTTCCGCCAGCaa                                                                            >  2:29960/1‑149 (MQ=255)                                                                             attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGG‑CAGGGGAATACA‑CCGCGAACAAAT‑AAAG‑CCCTTCCCCTTTTTTCGTTGCTGCCCCCAGCGGTCTGT‑CCTTTTCCGCCCGCAActct                                                                        >  1:691539/1‑149 (MQ=255)                                                                              ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGT‑CAGGGGAATTCA‑CCGCGAACAGAT‑ACAG‑CCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCCGCAActc                                                                         >  1:601176/1‑147 (MQ=255)                                                                                 ttCTCGTGAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTCTAGATCATCCCCGTGCGGGGGATTACA‑CC‑CGGCACGAG‑AAAA‑CCCCTCCGCCTTTTTCGTTGCTCTCGCCACCGCGCTGT‑CCTTTGTCGCCAGCAACTTTTCTATc                                                                  >  1:492410/1‑151 (MQ=255)                                                                                  tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGT‑CAGGGGAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTTCCGTTGCTGCCCCCAGCGTTCTGT‑CCTTTTCCCCCAGCAACTCTGCttt                                                                   >  2:541705/1‑147 (MQ=255)                                                                                  tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGCTTTTGAAGATCATAACGGG‑CAGGGTAATTCA‑CCCCGCCCAGAT‑ACAG‑CCCCTTCCCCTTTGCCGTTTCTCCCGCCCGCGTTCTTT‑CCCTTTCCGCCAGCaa                                                                            >  1:681980/1‑140 (MQ=255)                                                                                   ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGG‑CAGGGGAAACCA‑CCCCGACCAGAT‑ACAG‑CCCTTTCCCCTTTGCCGTTGCTGCCCCCCGCGTTCTGT‑CCTTTTCCCCCCGCAACTCTGCTAt                                                                   >  1:690888/1‑148 (MQ=255)                                                                                     cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGG‑TAGGGGAATACACCCCCGACCA‑AT‑ACAG‑CCCTTCCCCTTTCCCCGGTGGTGCCCGCCGCGTTCTGA‑TCTTTGCCGGCCACAACTCTGCTATc                                                                  >  1:793589/1‑147 (MQ=255)                                                                                          gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGG‑CAGGGGAATCCA‑CCGCGGCCAGAT‑AAAG‑CCCCTCCGCTTTTGCCGTTGCTGCCGCCCGCGTTCTGT‑CCTTTGCCGCCCGCAACTCTGCTCTCCAACGCTcc                                                         >  1:395142/1‑151 (MQ=255)                                                                                            ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCgg                                                       <  1:441124/151‑1 (MQ=255)                                                                                            ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCg                                                        <  2:468400/150‑1 (MQ=255)                                                                                            ccGCCAGAAATAGCGGGCCCATTGGCGGATTTTGAAAAACATACCCGG‑CAGGGTAATACA‑CCGCGACCAAAC‑AAAC‑CCCCTCCCGTTTTGGCGTTGTTGCCCCCAACGGTCTGTGCCTTTTCC‑CCCGCAAATCTCCTATccccgtcctc                                                         >  2:755716/1‑141 (MQ=255)                                                                                             cGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGc                                                      <  1:649580/151‑1 (MQ=255)                                                                                                   aaaTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGGAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTTCCGTTGCTGCCGCCCGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCCGCTgg                                                   >  2:499742/1‑148 (MQ=255)                                                                                                     aTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgtg                                              <  1:29773/151‑1 (MQ=255)                                                                                                      tAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGG‑CAGGGGAATCCA‑CCGCGGCCAGAT‑ACAG‑CCCCTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTTCCGCCCACCAATCTGCTATCCAGCTCTCCCGCTGGGTGTgg                                             >  1:480328/1‑151 (MQ=255)                                                                                                       aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTggg                                            <  1:720264/151‑1 (MQ=255)                                                                                                           ggCCCATTGGCGGTTTTGGGAGATCATACCGGG‑CAGGGTAATCCA‑CCGCGACCCGAT‑ACAG‑CCCTTTCGCTTTTTCCCTTGCTGCCCCCCGCGTTCTGT‑CCTTTTCCCCCCGCCACACTGCTATCCAGCTCTCCGGCTGGGTGTGGGCAcc                                        >  1:530659/1‑151 (MQ=255)                                                                                                                  tGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                                 >  1:722736/1‑151 (MQ=255)                                                                                                                    gCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCAt                               >  2:40258/1‑151 (MQ=255)                                                                                                                      ggTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa                             <  1:831089/151‑1 (MQ=255)                                                                                                                        ttttGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                           >  2:378391/1‑151 (MQ=255)                                                                                                                        ttttGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                           >  1:678171/1‑151 (MQ=255)                                                                                                                            ggAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                        <  2:21877/150‑1 (MQ=255)                                                                                                                              aaGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                     >  1:511105/1‑151 (MQ=255)                                                                                                                                gATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGAc                   >  2:805656/1‑151 (MQ=255)                                                                                                                                    aTACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat                >  2:199385/1‑150 (MQ=255)                                                                                                                                    aTACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat                >  1:720100/1‑150 (MQ=255)                                                                                                                                    aTACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGATTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAata               <  2:36886/151‑1 (MQ=255)                                                                                                                                          gT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg         >  2:14074/1‑151 (MQ=255)                                                                                                                                             cAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc       <  2:485972/151‑1 (MQ=255)                                                                                                                                             cAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc       <  2:105990/151‑1 (MQ=255)                                                                                                                                                 gTAATGCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat    >  1:86999/1‑150 (MQ=255)                                                                                                                                                  tAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCatat  >  2:205329/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                           CCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGT‑CAGGGTAATCCA‑CCGCGACCAGAT‑ACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434738‑2435032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑