Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,435 | 0 | C | T | 50.0% | 36.9 / 47.8 | 58 | intergenic (‑179/‑28) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (3/26); new base T (29/0); total (32/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.30e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA > NC_000913/3206287‑3206583 | gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCa < 1:664350/151‑1 (MQ=255) aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGAATTTTCTAGa > 2:887497/1‑149 (MQ=255) gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATAc < 1:432883/151‑1 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTcaaacaaaaa > 2:735696/1‑146 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAATACa > 2:285185/1‑150 (MQ=255) aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACaaa < 1:487103/151‑1 (MQ=255) aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACaaaa > 2:816014/1‑150 (MQ=255) aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACt < 1:697415/151‑1 (MQ=255) cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGt < 2:398888/151‑1 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGcc < 1:174821/151‑1 (MQ=255) cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCC‑GACCAAAACAAAAAAAGG‑AGGTGCCa > 2:865729/1‑150 (MQ=255) ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACAAAAAATGG‑AGTTGGCatg > 2:408212/1‑147 (MQ=255) tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAATTTTTTCTGAAAAATAAAAAAAATGG‑AGTTGCCatgatgtg > 1:879333/1‑149 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCg < 1:345909/151‑1 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCg < 2:463123/151‑1 (MQ=255) gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAAAACAAAAACTGG‑AGTTTGCatggtggggg > 1:201924/1‑145 (MQ=255) gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGaa < 2:567657/151‑1 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGtaataa < 2:495739/151‑1 (MQ=255) tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGc < 2:201953/150‑1 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATAAAAAAAATTG‑AGGTGCCATGATGAGCGAAAGTAATAAACaa > 2:179597/1‑149 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACa < 2:499105/150‑1 (MQ=255) aTTTTCGCGTTGATTCACGAACTATTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTAGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAg < 2:597233/151‑1 (MQ=255) tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa < 1:643543/150‑1 (MQ=255) gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa < 2:425675/151‑1 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACAAAAAATGG‑AGTTGCCATGATGAGGGAAAGGAATAAACAAAAGGGAGTGAATaa > 2:780374/1‑150 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAATTTTTTCAAACAAAAAAAAAAAAAGG‑AGGTTCCATGATGAGGGAAAGGAAAAAAAAAAAGGCAGTGAaaaa > 1:202426/1‑150 (MQ=255) cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg < 1:132624/151‑1 (MQ=255) cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAc < 1:159456/151‑1 (MQ=255) gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAATACAAAAAATGG‑AGGTGCCATGATTAGGGAAAGTAATAAAAAACAAGCAGGGGATAAGGTGAc > 2:99539/1‑150 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTCTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGG‑AGGTGCCCTGATGAGCGGAAGTAATAAACAACAAGCAGGGGATAAAtt > 2:393676/1‑146 (MQ=255) tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt < 2:310439/150‑1 (MQ=255) aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAATACAAAAACTTG‑AGTTGCCATGATTAGCGGAAGTAATAAACAACAGGCAGTGAATAAGGTGACAGAGAATGTCGc > 2:225081/1‑150 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa < 1:874920/149‑1 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc < 2:882181/151‑1 (MQ=255) tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAATTTTTTCAGACAAAAACAAAAAATGG‑AGTTGCCATGATGAGGGAAAGAAATAAACAAAAAGCAGGGAATAAGTTGACAGAGAATGTCGCTAACTTTACCGcc > 2:481130/1‑151 (MQ=255) ggCAAAAAATATAAAACCACCTTTTTAGTGGTAGTTTGTGGAGGC‑CAATTTTTTCAAACAAATACAAAAAATGGCAGTGGTAAT‑ATTAGCGACAGGAATAAAAAACAAGGAATGGATAAGAAGAAAGAGACTGTAGATaaacttaattctc > 2:825225/1‑140 (MQ=255) gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAAAACAAAAAATGG‑AGGTGCCATGATGAGGGGAAGTAATAAACAACAAGCAGGGAAAAAAATGACAGAGATTGTCGCTAAATTTaacccc > 1:75366/1‑144 (MQ=255) aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGG‑GGGTGGCATGAGGGGGGGAAGAAATAAACAACAGGCAGTGAATAAAATGAAAAAGATTGTCGCTAACATTACAGCCATg > 1:317702/1‑149 (MQ=255) aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATAAAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt < 1:34725/151‑1 (MQ=255) aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGG‑AGGTGCCATGGTGAGCGGAAGGAATAAACAACAAGCAGTGAATAAGTTGACAGAGATTTTTGCTAACTTTACCGCCATGAtt > 2:806749/1‑151 (MQ=255) tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTTCAAAAAAAAAAAAAAATGG‑GGGTGGCATGATGGGGGAAAAAAAAAAACAAAAAGCAGGGAAAAAATTGGCAGAGAATTTTGCTTAATTTTTCGCCCTGAttt > 1:416360/1‑148 (MQ=255) tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAATTTTTTCAGACAAAAACAAAAAAATG‑GGTTGCCCTGATGAGCGGAAAAAATAAACAACAAGCAGAGAAAAAGTTTACAGAGAATGTTGCTTACTTTACCCCCATGaataaa > 2:575609/1‑145 (MQ=255) ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCCAACCAATTCAAAAAATGG‑GGTTTCCTTGATGAGCGAAAGGAATAAAGCACAGGGAGGGAATTAATTGGAACGGATTGTTGCTAAATTTTcccgcccgattttt > 1:118229/1‑137 (MQ=255) ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGG‑AGGTGCCATGATGAGCGGAAGGAATAAACAAAAGGCAGTGAAAAAATTGACAGAGAATGTCGCTAAATTTAACGCCATGATTTCt > 2:216005/1‑149 (MQ=255) ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑AAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAtt < 1:575567/146‑1 (MQ=255) aaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCAACAAAAACAAAAAATTG‑AGTTGGCATGGTGGGGGGAAGTAAAAAACAAAAAGGAGGGAAAAAAGTGACAAAGATTGTCGCTTAATTTAACGCCCTGAttttttacc > 1:11331/1‑144 (MQ=255) aaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGG‑AGTTGCCATGATGGGGGGAAGGAATAAGGAACAGGGAGTGGATAAGATGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAccc > 2:187663/1‑150 (MQ=255) aaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGG‑AGGTGCCATGATGAGCGAAAGGAATAAACAAAAAGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTTccc > 2:780153/1‑150 (MQ=255) aaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAAGAAAACAAAAAAGTG‑AGTGGTGCTGATGTGGGAAAGGAATAACCAAAAAGCGGTGAATAAGGTGAGAGAGAGTTTCGCTAAAATTAGCGCCATGAtttattcacc > 2:494247/1‑144 (MQ=255) aaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTCC‑GACAAAAAAAAAAAATGG‑AGTTGCCCTTATGAGGGGAAGTAATAAGCAAAAGGCAGTGAAAAAGGTTAAAGAGATTGTCGCTAACTTTACCGCCCTGGTTTTTAacc > 2:801671/1‑148 (MQ=255) aCCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAATTTTTTCATACAAAAACAAAAACTGG‑AGGTTGCATGATGGGCGGAAGGAAAAAACAAAAAGCAGTGAATAAAATGACAGAGAATTTCGCTAAATTTTTCCCCCTGATTTTTatc > 1:568401/1‑145 (MQ=255) ccACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCAAGAAAAATAAAAAAACTGG‑AGTTGCCATGATGAGCAAAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt < 2:276822/151‑1 (MQ=255) cacaTTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGGCAAAAACAAAAAATGG‑AGTTGGCCTGGTGGGGGAAAGTAATAAGCAACAAGCAGTGAATAAATTTACAGAGATTTTCGCTAACTTTTACGCCCTTATTTTTAcccccatg > 1:471570/1‑146 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAATACAAAAAATGG‑GGTTGCCATGATGAGCGAAAGGAATAAACAACAGGCAGGGAATAAATTGACAGAGATTGTTGCTAACTTTACCGCCATGATTTTTACCCGaa > 2:84166/1‑148 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGc < 2:394080/150‑1 (MQ=255) tttAGTGGTAGTTTGTGGCGGG‑GAATTTTTTCCGAAAAAAAAAAAAACTGG‑GGTTGCCATGATGAGGGAAAGTAATAAGCAACAAGGAGTGAATAAATTGTAAGAGAATGTCGGTAAATTTACCCCCCTGAttttttacccaa > 2:216573/1‑134 (MQ=255) ggTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 2:485576/151‑1 (MQ=255) ttCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa < 2:207599/150‑1 (MQ=255) ccAGACAAATACAAAAACGGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa < 2:885837/149‑1 (MQ=255) | GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGG‑AGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA > NC_000913/3206287‑3206583 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |