Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	461,034	T→G	I716S (ATC→AGC)	lon →	DNA‑binding ATP‑dependent protease La

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	461,034	0	T	G	100.0%	179.1 / NA	49	I716S (ATC→AGC)	lon	DNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/27);  total (22/27)

ACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGG  >  NC_000913/460887‑461180                                                                                                                                                    |                                                                                                                                                   acgtACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCgg                                                                                                                                                 <  1:347377/151‑1 (MQ=255)   gtACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCggtg                                                                                                                                               >  1:443506/1‑151 (MQ=255)         gAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAAGGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTga                                                                                                                                         <  2:91501/151‑1 (MQ=255)           aGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTgaaa                                                                                                                                       <  2:834829/151‑1 (MQ=255)           aGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTgaaa                                                                                                                                       <  1:468745/151‑1 (MQ=255)               gCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTgaaagaaa                                                                                                                                   <  1:350392/151‑1 (MQ=255)               gCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTgaaagaa                                                                                                                                    >  1:557607/1‑150 (MQ=255)                 gACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGaaaa                                                                                                                                  >  1:139472/1‑150 (MQ=255)                   cGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACt                                                                                                                               <  1:434975/151‑1 (MQ=255)                      cGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGGCCGGTGAGATCACTCTGCGTGGTCCGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTc                                                                                                                              <  1:169618/149‑1 (MQ=255)                       gAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTg                                                                                                                           <  2:162894/151‑1 (MQ=255)                         aaGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGc                                                                                                                         <  1:350382/151‑1 (MQ=255)                             tGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAgcg                                                                                                                     >  1:594604/1‑151 (MQ=255)                                     gTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCgcgg                                                                                                             <  1:528094/151‑1 (MQ=255)                                       gCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCgcggcg                                                                                                           <  1:177974/151‑1 (MQ=255)                                             aTTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTa                                                                                                     <  2:91370/151‑1 (MQ=255)                                               agCTATGTGCACCGCGCTGGTTTCTTGACTGACCGGAAACCCGGTTCGTGCCGATGCGGCAATGACCGGGGAGATCACTCTGCGGGGTCAGGTACTGCCGAGCGGGGGTTTGAACGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTa                                                                                                     >  1:710063/2‑149 (MQ=255)                                                   aTGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAg                                                                                               <  2:439544/151‑1 (MQ=255)                                                           cgcgCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAAtt                                                                                       <  2:393062/151‑1 (MQ=255)                                                             cgcTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTcc                                                                                     <  2:123398/151‑1 (MQ=255)                                                                tGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTcc                                                                                     <  1:575335/148‑1 (MQ=255)                                                                 ggTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTc                                                                                 >  2:255358/1‑151 (MQ=255)                                                                    ttCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGa                                                                               <  2:689631/150‑1 (MQ=255)                                                                      cTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGaaa                                                                             >  2:832043/1‑150 (MQ=255)                                                                        tGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAAt                                                                           >  2:774765/1‑150 (MQ=255)                                                                            tGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAAcg                                                                      >  2:730171/1‑151 (MQ=255)                                                                               ccGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGa                                                                   <  2:41281/151‑1 (MQ=255)                                                                                            tCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGAtt                                                      >  1:44785/1‑151 (MQ=255)                                                                                              gTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTcc                                                    >  2:277695/1‑151 (MQ=255)                                                                                              gTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGCGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTcc                                                    >  2:761961/1‑151 (MQ=255)                                                                                                gCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCt                                                   <  1:175517/150‑1 (MQ=255)                                                                                                     tgtgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACaa                                              >  2:90740/1‑150 (MQ=255)                                                                                                       tgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGt                                           >  2:111030/1‑151 (MQ=255)                                                                                                        ggCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGt                                           <  1:690191/150‑1 (MQ=255)                                                                                                         gCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGAAAGAGATTCCTGACAACGTa                                          >  1:671027/1‑150 (MQ=255)                                                                                                          cAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGGAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAAt                                        >  2:618458/1‑151 (MQ=255)                                                                                                            aTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTg                                      <  2:631024/151‑1 (MQ=255)                                                                                                                 cGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGAt                                 <  1:798055/151‑1 (MQ=255)                                                                                                                   gTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCt                               <  2:713370/151‑1 (MQ=255)                                                                                                                    tGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTg                              >  1:204290/1‑151 (MQ=255)                                                                                                                      agaTCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTgg                             >  2:622736/1‑150 (MQ=255)                                                                                                                       gaTCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGat                           <  1:216554/151‑2 (MQ=255)                                                                                                                           aCTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTc                       >  2:503274/1‑151 (MQ=255)                                                                                                                           aCTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTc                       <  1:694853/151‑1 (MQ=255)                                                                                                                                gCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATc                    <  1:2732/149‑1 (MQ=255)                                                                                                                                       cAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgc           >  1:17845/1‑151 (MQ=255)                                                                                                                                        aGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgcg          >  1:731624/1‑151 (MQ=255)                                                                                                                                         ggTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgcgc         <  2:258779/151‑1 (MQ=255)                                                                                                                                                 cGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAgg  >  2:556993/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                   ACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGG  >  NC_000913/460887‑461180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑