Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,810,459 T→C V39A (GTA→GCA)  ygaH → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,4590TC100.0% 179.7 / NA 49V39A (GTA→GCA) ygaHputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

AATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAACACG  >  NC_000913/2810315‑2810606
                                                                                                                                                |                                                                                                                                                   
aaTCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCTTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGAAt                                                                                                                                               >  1:307627/1‑151 (MQ=255)
     aGGCATTCTGGCATGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTTCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCTCGGCAGGTATTTTGc                                                                                                                                          <  2:468062/151‑1 (MQ=255)
         aTTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCg                                                                                                                                       >  1:426678/1‑150 (MQ=255)
              ggCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACAcc                                                                                                                                  >  1:648416/1‑150 (MQ=255)
               gCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCAt                                                                                                                                <  2:498382/151‑1 (MQ=255)
               gCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCAt                                                                                                                                <  1:775351/151‑1 (MQ=255)
                cAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTCCTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCAtt                                                                                                                               >  1:771460/1‑151 (MQ=255)
                cAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGGATTTTGCTCGACACCAtt                                                                                                                               >  2:447679/1‑151 (MQ=255)
                        gcCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGc                                                                                                                       <  2:787466/151‑1 (MQ=255)
                            gATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCg                                                                                                                   <  2:38110/151‑1 (MQ=255)
                             aTGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGa                                                                                                                  <  2:353904/151‑1 (MQ=255)
                               gAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGat                                                                                                                 <  2:438767/150‑1 (MQ=255)
                                      gAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGct                                                                                                          <  1:529299/150‑1 (MQ=255)
                                               ctgctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGtt                                                                                                 >  1:614844/1‑150 (MQ=255)
                                                 gctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTg                                                                                                <  1:551251/149‑1 (MQ=255)
                                                   ttGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGtctc                                                                                            >  1:522084/1‑151 (MQ=255)
                                                    tGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGtct                                                                                             <  1:107740/149‑1 (MQ=255)
                                                     gggTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTctct                                                                                           <  1:447779/150‑1 (MQ=255)
                                                     gggTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGGATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTctct                                                                                           >  1:699551/1‑150 (MQ=255)
                                                       gTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAcc                                                                                        <  2:58545/151‑1 (MQ=255)
                                                            tAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCCTCGCCTCGCTATGCGCTCTGCTGGTTGTCTCTACCGCAcc                                                                                   >  1:133988/1‑151 (MQ=255)
                                                            tAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCAcc                                                                                   >  2:568922/1‑151 (MQ=255)
                                                                 ggcgtggcgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAg                                                                              <  1:115004/151‑1 (MQ=255)
                                                                 ggcgtggcgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAg                                                                              <  2:680402/151‑1 (MQ=255)
                                                                   cgtggcgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTg                                                                            <  2:519029/151‑1 (MQ=255)
                                                                    gtggcgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTg                                                                            >  2:536671/1‑150 (MQ=255)
                                                                        cgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCa                                                                       <  2:275246/151‑1 (MQ=255)
                                                                          aaTTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCAc                                                                      >  2:131595/1‑150 (MQ=255)
                                                                                tGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATaca                                                                <  2:836453/150‑1 (MQ=255)
                                                                                 gCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACg                                                              <  2:62148/151‑1 (MQ=255)
                                                                                       gCTATTGGCCCCTGCGCCGGCGTGTGGGTAATGCTCGCCCGACCAAACGTGGCGCGGCAGTTATTTTACTCGCTACCATTGGCATCGCCTCGATATGCACTCGGCTGGTTGTCTCTACCGCCCCAGAAGTTATGCACGATACACGCCGttt                                                        <  1:159629/151‑1 (MQ=255)
                                                                                          aTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCg                                                      >  2:34303/1‑150 (MQ=255)
                                                                                           tttGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGACATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTc                                                       <  1:346954/148‑1 (MQ=255)
                                                                                                 gCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACg                                              >  1:217564/1‑151 (MQ=255)
                                                                                                       ccTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGt                                         <  2:129113/150‑1 (MQ=255)
                                                                                                       ccTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGt                                         <  2:876066/150‑1 (MQ=255)
                                                                                                          gCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCgg                                      <  2:51904/150‑1 (MQ=255)
                                                                                                                  gTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGt                             <  2:125737/151‑1 (MQ=255)
                                                                                                                   tAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGt                             >  1:656870/1‑150 (MQ=255)
                                                                                                                     aTGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACt                          >  2:10300/1‑151 (MQ=255)
                                                                                                                         ccgcccAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGt                      <  1:47932/151‑1 (MQ=255)
                                                                                                                          cgcccAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTg                     <  2:795312/151‑1 (MQ=255)
                                                                                                                           gcccAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGc                    >  2:668057/1‑151 (MQ=255)
                                                                                                                               aaccaaACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGt                >  1:563876/1‑151 (MQ=255)
                                                                                                                                 ccaaACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGttt              <  2:886963/151‑1 (MQ=255)
                                                                                                                                     aCGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCtat          >  1:498223/1‑151 (MQ=255)
                                                                                                                                        tGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATaa        >  2:403447/1‑150 (MQ=255)
                                                                                                                                            gcgGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTCCCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAac     <  1:122406/149‑1 (MQ=255)
                                                                                                                                             cgGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAACACg  >  1:261143/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAACACG  >  NC_000913/2810315‑2810606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑