Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,056	0	G	A	46.2%	68.8 / 39.5	65	intergenic (‑119/+84)	ves/spy	cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (30/5);  new base A (0/30);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.94e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824909‑1825205                                                                                                                                                    |                                                                                                                                                      gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                                    <  2:1076931/151‑1 (MQ=255)   cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                                  <  2:545309/151‑1 (MQ=255)   cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                                    >  1:146515/1‑149 (MQ=255)      tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaa                                                                                                                                                <  1:416329/150‑1 (MQ=255)       ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaaa                                                                                                                                               >  2:419914/1‑150 (MQ=255)        tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAc                                                                                                                                             >  1:841615/1‑151 (MQ=255)          cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAcc                                                                                                                                            <  2:844402/150‑1 (MQ=255)           gCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgc                                                                                                                                          >  2:433839/1‑151 (MQ=255)            cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                           <  2:2304/149‑1 (MQ=255)              tatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgcc                                                                                                                                         >  2:379415/1‑149 (MQ=255)               atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGAt                                                                                                                                      >  2:958436/1‑151 (MQ=255)                tCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTACCCGTGGATGGTTATCGGCAACTCGGGTAATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                                       <  1:467554/149‑1 (MQ=255)                       aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                               >  2:933178/1‑150 (MQ=255)                         tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                            >  1:265498/1‑151 (MQ=255)                         tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                            >  2:1118217/1‑151 (MQ=255)                          acATACTCGCTCCCGTCCCGGTCTTTTCATTGTTATCGTTCAGGTCACCGTGGATGGTTATCGGCAACTCGGGTGTTTTACGCTAGGGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGCTCCTGTCCAc                                                                                                                             >  1:129825/2‑149 (MQ=255)                           cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                          >  1:411209/1‑151 (MQ=255)                              cctcccccccGCCCCGTTTTTTTCATTTTTTTCTTACAGGAAACGGGGGAGGTTTAGCGCAAACTGGGGTGATTAACGTTGGTGGTGATCGGGTTTTTTTTAAAATTGTTGGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                        <  1:520051/143‑1 (MQ=255)                               cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                       >  1:772036/1‑150 (MQ=255)                                tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCGTTAc                                                                                                                     >  1:359225/1‑151 (MQ=255)                                 cGCTCCGGGCCGTTTCTTTTCATTTTTATCGTTCAGGTACCCGTGGTTGTTTACCGCCAGGTCGGGTGTTTTATGTTGGGGGTGACAGGGTTTTTTTTACATTTGTTTGAGAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                        <  2:100690/147‑1 (MQ=255)                                  gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                   >  2:425072/1‑151 (MQ=255)                                   cTCCCGCCCGTTTTTTTTTTTTGTTTTCGTTCGGTTAACGTTGGTTGGTTATCGCCAACTGGGTTGTTTTCCGTTAGTGGTTAGCGGGTTTTTTTTACACCTTTTGGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCa                                                                                                                         <  2:201808/144‑1 (MQ=255)                                     cccGGCCCTTTTTTTTCATTGTTATCGTTCAGGTACCCGTGGATGGTTATCGGCAACTCGGGTGTTTTCCGTTGGGGGTGATCGGGTTTTTTTTACACTTGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                                 <  2:52598/150‑1 (MQ=255)                                      ccGGCCCGTTCTTTTAATTGTTATCGTTCAGGTAACGGGGGATGGTTAGCGGAAACGGGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAg                                                                                                               <  2:976489/151‑1 (MQ=255)                                        ggCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGTTGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAg                                                                                                               >  2:867473/1‑149 (MQ=255)                                         gCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                             >  2:133879/1‑150 (MQ=255)                                           ccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt                                                                                                          >  1:532020/1‑151 (MQ=255)                                              ttCTTTTCATTTTTTTCGTTCTGGTACCCGTGGATGGTTATCGCAAACTCGGTTTATTTCCGTTAGTGGTGATCAGGTTTTTTTTACATCTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                        <  1:834211/150‑1 (MQ=255)                                                cTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                      >  2:892613/1‑150 (MQ=255)                                                     cATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                >  2:50929/1‑151 (MQ=255)                                                               gTTCAGATACCGGTGGATGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCGGTTTTTTTTTACATTTTTTGAAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                           <  2:368166/146‑1 (MQ=255)                                                                  agggaaacgggggAGGGTTTTCGGCACTGGGGGTGATTTAGGTTGGTGGTGATCAGGTTTTTTTAACACTTTTTAGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGt                                                                                   <  2:587972/140‑1 (MQ=255)                                                                           gTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                           >  1:436823/1‑150 (MQ=255)                                                                             ggATGTTTACCGGCACCGGGGTTGATTTACGTTGGTGGTGTTCAGGTTTTTTTTACAACTTTTAGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                            <  2:421002/147‑1 (MQ=255)                                                                             ggATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg                                                                         >  1:352452/1‑150 (MQ=255)                                                                                   ttaccgcaaaCTGGGTTGATTTACTTTAGTGGTGATCAGGTTTTTTTAACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                                   <  1:297235/142‑1 (MQ=255)                                                                                   ttATCGCCAACTCGGGTGATTTACGTTGGTGGTAATCAGGTTTTTTTTACACTTTTTTAAGAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg                                                                     <  2:560012/148‑1 (MQ=255)                                                                                   tagtcgccacccggggTGTTTTACTTTGGTGGTGATCAGCTTTTTTTTCAAAATGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg                                                                         <  1:79234/131‑1 (MQ=255)                                                                                    tttCGGCAACTGGGGTGATTTACTTTGGTGGTGATCGGTTTTTTTTTACAATTTTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                  <  1:975509/148‑1 (MQ=255)                                                                                    tATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGTTTTTTTTTAAATCTGTTGGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                  <  2:855541/150‑1 (MQ=255)                                                                                        gccaaccggggTGATTTCGTTTGGGGGTAATCAGTTTTTTTTTACACGTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatta                                                                 <  1:251247/139‑1 (MQ=255)                                                                                         gCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                               >  1:211088/1‑148 (MQ=255)                                                                                                     ttttACGTTGTTGGTTATCAGGTTTTTTTTAAAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAg                                                   <  2:21199/147‑1 (MQ=255)                                                                                                     aTTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAgg                                                  >  1:487683/1‑149 (MQ=255)                                                                                                      tttAGGTTGGGGGTGATCAGGTTTTTTTTACAACTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                                <  2:494859/150‑1 (MQ=255)                                                                                                      tttACTTTAGTGGTGATCAGTTTTTTTTTACAGCTGTTAGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGc                                                 <  1:314500/149‑1 (MQ=255)                                                                                                       ttACGTTAGTGGTGATCAGGTTTTTTTTAAAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAt                                               <  1:480147/150‑1 (MQ=255)                                                                                                         actttAGTGGTTATCAGTTTTTTTTTACAATTGTTGGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttg                                             <  1:784897/147‑2 (MQ=255)                                                                                                            ttggtggtgATCAGGTTTTTTTTACATTTGTTTAAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                           <  2:1070231/146‑1 (MQ=255)                                                                                                            ttAGTGGTGATCAGTTTTTTTTAACAACTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                          <  2:841682/150‑1 (MQ=255)                                                                                                            ttAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                          <  2:218704/150‑1 (MQ=255)                                                                                                              aGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                       >  2:1095431/1‑151 (MQ=255)                                                                                                              aGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                       >  2:722323/1‑151 (MQ=255)                                                                                                                 ggtgATCAGGTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                            <  2:194314/143‑1 (MQ=255)                                                                                                                  gtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                     >  1:744988/1‑149 (MQ=255)                                                                                                                       cAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCCGGCATTTTTCCTTTTTCCGc                                >  1:224045/1‑149 (MQ=255)                                                                                                                       cAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCt                               >  1:968163/1‑150 (MQ=255)                                                                                                                        ggtttttttttACAATTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCt                               <  1:312436/145‑1 (MQ=255)                                                                                                                            ttttttaACACTTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg                          <  1:746214/150‑1 (MQ=255)                                                                                                                                ttaacacCTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                      <  1:279031/147‑1 (MQ=255)                                                                                                                                  tACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                    >  1:560268/1‑150 (MQ=255)                                                                                                                                   aCAACTGTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                  <  2:1013156/151‑1 (MQ=255)                                                                                                                                   aCAACTGTTAGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                      <  1:1055052/147‑1 (MQ=255)                                                                                                                                      attttttGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc               <  2:596183/147‑1 (MQ=255)                                                                                                                                        tGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTTTTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAg                 >  1:57/1‑147 (MQ=255)                                                                                                                                         gTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt            >  1:28167/1‑151 (MQ=255)                                                                                                                                          tttGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc               <  2:411248/147‑1 (MQ=255)                                                                                                                                          tttGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc               <  1:754586/147‑1 (MQ=255)                                                                                                                                           ttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGTAGGCATTTTACCTTTTGACGCTGGACGGTCTGTCAGACGCtt           >  2:401928/1‑150 (MQ=255)                                                                                                                                             gagaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtct         >  2:356363/1‑150 (MQ=255)                                                                                                                                              agaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa       <  1:58812/151‑1 (MQ=255)                                                                                                                                               gaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa       >  2:286589/1‑150 (MQ=255)                                                                                                                                                aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaa      >  1:995259/1‑150 (MQ=255)                                                                                                                                                aagaaaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa     <  2:548869/151‑1 (MQ=255)                                                                                                                                                   agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGGAAGATTATTCAGCCGTTGCAGGCCTTTTACCTTTTGCCGCTGGACGGTCTGTCAGACGCCTCTCAAAAtt  >  1:839764/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824909‑1825205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑