Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,346,675 | 0 | T | G | 43.5% | 143.8 / 18.0 | 85 | T218P (ACC→CCC) | fumB | anaerobic class I fumarate hydratase (fumarase B) |
Reads supporting (aligned to +/- strand): ref base T (9/39); new base G (37/0); total (46/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.20e-16 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTC > NC_000913/4346527‑4346822 | aaCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑c < 2:1084683/151‑1 (MQ=255) aaCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑c < 1:457477/151‑1 (MQ=255) gCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCGGGGGTCAGCACCGAGGGGA‑c > 1:397202/1‑148 (MQ=255) gACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGGGGGTGCAGGACCGCGGGGA‑AGCActtt > 1:736433/1‑151 (MQ=255) gACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCAttt < 1:571233/150‑1 (MQ=255) cATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCGGCCGGACCCAGGGGA‑CGCAtttt > 1:1080081/1‑148 (MQ=255) gttcgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGA‑CGCCTTTTTTcgac > 1:418852/1‑151 (MQ=255) ttcgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGTACCGAGGGGA‑CGCCTTTTCTcgac > 1:1102495/1‑150 (MQ=255) ttcgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTcgac < 2:989688/150‑1 (MQ=255) cccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAg < 2:467836/151‑1 (MQ=255) cccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACGAGGGGA‑CGCATTTTTTTGGCGAgg > 1:47183/1‑148 (MQ=255) ccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGTACCGAGGGGA‑CGCATTTTTTCGACGAgg > 2:580742/1‑147 (MQ=255) ccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGTACCGAGGGGA‑AGCACTTTTTCGACGAGGGAg > 1:361820/1‑150 (MQ=255) ccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGt < 1:870572/151‑1 (MQ=255) cTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGCGCCGTACCGAGGGGA‑CGCATTTTTTCGACGAGGGAGtt > 1:457072/1‑151 (MQ=255) cGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGGGGGAGTACCGGGGGGA‑CGCATTTTTTCGACGAGGGAGgtttt > 1:242829/1‑150 (MQ=255) cGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGA‑CGCATTTTTTCGACGAGGAAGgttttt > 2:635780/1‑150 (MQ=255) gTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGA‑CGCATTTTTTTGACGAGGGAGgtttt > 1:1073719/1‑149 (MQ=255) tCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCa < 2:540121/150‑1 (MQ=255) gCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCCGCAGTACCCAGGGGA‑CGCATTTTTTCGACGAGGGAGTTTTTTTGttt > 1:347066/1‑151 (MQ=255) tCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCg < 2:403706/150‑1 (MQ=255) tCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGGACCGCGGGGA‑CGCATTTTCTCCACGAGGGGGGTTTTTAGTTTTCCgg > 1:907357/1‑151 (MQ=255) aTCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGGTGGAGAACCCAGGGGA‑CGCCTTTTTTCGAGGAGGGAGtttttttagtttccggg > 1:1113807/1‑138 (MQ=255) gTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGACCGAGGGGA‑CGCATTTTTTCGACGAGGAAGGTTTTTAGTTTTCCggggg > 2:91141/1‑147 (MQ=255) gTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGA‑CGCAATTTCTCGACGAGGGAGGTTTTTTGTTTGCCGGGAg > 2:577178/1‑149 (MQ=255) tAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGAACGAGGGGA‑CGCATTTTTTCGACGAGGAAGGTTTTTAGGTTTCCGGGGGTcagc > 2:89189/1‑150 (MQ=255) aGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTcagca‑g < 2:408599/151‑1 (MQ=255) aGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTcagca < 2:75396/150‑1 (MQ=255) gTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑gg < 1:421047/151‑1 (MQ=255) gAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCCGTACCGCGGGGG‑CGCATTTTCTCGGCGAGGGAGTTTTTTTGTTTGCCGGGGGGCAGCA‑ggg > 1:734660/1‑150 (MQ=255) gcgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGCGGGGA‑CGCATTTTTTCGACGAGGGAGGTTTTTAGTTTGCCGGGGGGCAGCA‑ggg > 1:931533/1‑148 (MQ=255) gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTg < 1:635717/151‑1 (MQ=255) gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTg < 1:956480/151‑1 (MQ=255) tGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGt < 1:218581/150‑1 (MQ=255) gCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGAGGGTA‑AGCATTTTTTTGACGAGGAAGGTTTTTAGTTTGCCGGGAGGCAGCA‑GGggtttggttt > 2:31068/1‑151 (MQ=255) aaCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCCAGGGGA‑CGCATTTTCTCGACGAGGAAGGTTTTTAGTTTTTCGGGGGTCAGCA‑GGGCTTTGGgttt > 1:1083465/1‑148 (MQ=255) aaCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTc < 1:1014980/149‑1 (MQ=255) aaCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACCAGGGGA‑CGCATTTTTTCGACGAGGAAGTTTTTTTGTTTTCCGGGAGGCAGAAGGGGTTTTGGTTTc > 2:270962/1‑150 (MQ=255) acgtGACGGTGTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGTGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCt < 1:18123/147‑1 (MQ=255) ttGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGGAACGAGGGTA‑CGCATTTTTTCGACGAGGAAGGTTTTCAGTTTGCCGGGGGGCAGGA‑GGGGTTTGGGTTTCTgg > 2:510881/1‑150 (MQ=255) tGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGTACCGAGGGTG‑CGCATTTTCTCGACGAGGGAGGTTTTCAGGTTGCCGGGGGTCAGCC‑GGGGTTTGGTTTTCt > 2:386051/1‑147 (MQ=255) aCGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTaga < 1:91120/151‑1 (MQ=255) ggTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGAGGGGA‑CGCATTTTTTCGACGAGGGAGGTTTTCCGTTTGCCGGGGGGCAGCA‑GGGGTTTTGGTTTCTgg > 2:436697/1‑145 (MQ=255) gTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGGGGGGA‑CGCCTTTTTTTGACGAGGGAGTTTTTTCGTTTTCCGGGGGTCAGCA‑GGGCTTTTGTTTTCTGGTagag > 2:451050/1‑149 (MQ=255) gTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTGGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGAt < 1:612360/151‑1 (MQ=255) ttttCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTTCCGGGGGGA‑CGCCTTTTCTCCGCGAGGAAGTTTTTCATATTGCCCGGGGTCAGCA‑GGGCttttggtt > 2:1103884/1‑133 (MQ=255) aGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGGAGTGCCGAGGGGG‑AGCATTTTCTCCGCGAGGGGGTTTTTTAGTTTTCCGGGGGGGAGCA‑GGGGTTTGGTTTTCCGGTAGGGATACGttt > 1:128778/1‑151 (MQ=255) gttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGTAACGAGGGGA‑CGCATTTTTTCGACGAGGGAGGTTTTTAGTTTGCCGGGGGTCAGCA‑GGGCTTTTGGTTTCTTTTAGAGATACGtttt > 2:320551/1‑150 (MQ=255) gttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCGGCAGTTCCCAGGGGAGGGCATTTT‑TTGGCGGGGGAGTTTTTTAGTTTGTCGGGAGTCAGGA‑GGGCTTTGGGTTTCTGGTGGAGATTCGtttt > 1:460504/1‑150 (MQ=255) gttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTttg < 1:495321/151‑1 (MQ=255) ttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGGACCGAGGGGA‑CGCATTTTTTCGACGAGGAAGTTTTTTAGTTTGCCGGGGGGCAGCA‑GGGGTTTGGTTTTCTGGTAGAGATACGtttt > 2:328686/1‑149 (MQ=255) ggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTttgtt < 2:69508/150‑1 (MQ=255) gttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTgg < 1:83208/151‑1 (MQ=255) gttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGA‑CGCATTTTTTCGACGAGGAAGGTTTTTAGTTTGCCGGGGGGCAGCA‑GGGGTTTGGTTTCCTGGTAGAGATACGTTttgtt > 2:155011/1‑149 (MQ=255) tCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCa < 1:362075/150‑1 (MQ=255) ccGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCaga < 2:1094730/151‑1 (MQ=255) cGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCagag < 1:342445/151‑1 (MQ=255) cGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGA‑CGCATTTTTTCGACGAGGAAGGTTTTTAGTTTTCCGGGGGTCAGCA‑GGGCTTTGGTTTTCTGGTAGAGATACGTTTTGTTGGCag > 2:510615/1‑149 (MQ=255) cAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGGCGCAGTACCGGGGGGG‑CGCAATTTTTCGGCGAGGGAGGTTTTTAGTTTGTCGGGAGGCAGCAGGGGTTTTGGTTTCTTGG‑AGAGGGACGGTTTGTTGGGag > 1:712167/1‑147 (MQ=255) gACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGA‑CGCATTTTCTCCACGAGGAAGGTTTTTAGTTTGTCGGGGGGCAGCA‑GGGGGTTGGTTTTCTGGGAGAGATACGTTTTGTTGGGagag > 1:38286/1‑147 (MQ=255) cGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGTACCGCGGGGA‑CGCATTTTTTTGACGAGGAAGTTTTTTAGTTTGCCGGGGGGCAGCA‑GGGGTTTGGGTTTCTGGTAGGGATACGGTTTTTTTGCCGAggcgcc > 1:607121/1‑150 (MQ=255) aCCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCt < 2:640645/151‑1 (MQ=255) ccGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCtt < 2:991128/151‑1 (MQ=255) cGCCAATCACAAACGCGATATGGTACGGCGGGCCAGGTGCAGTAACGAGGGGA‑CGCATTTTTTCTACGGGGAAGTTTTTAAGTTTGTCTGGAGTCAGCA‑GGGGTTTGGTTTTCTGGGAGGGATACGTTTTTTTTGGAGAgccgccgcc > 2:400329/1‑148 (MQ=255) cAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGAACCGAGGGGA‑CGCATTTTCTCCACGAGGAAGTTTTTTAATTTGCCGGGGGGCAGCA‑GGGGTTTGGTTTCCTGGTAGAGATAAGGTTTTTTGGCAGAGGCGCCCCCCTTtgc > 2:675386/1‑151 (MQ=255) tCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTTTCGACGAGGAAGTTTTTTAGTTTTCCGGGGGGCAGCA‑GGGCTTTGGGTTCCTGGTAGAGATACGTTTTTTTTGCAGAGCCGCCGCCTTTcgcaa > 2:870623/1‑150 (MQ=255) caAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaacgca < 2:713111/151‑1 (MQ=255) aaaCGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaacgcaa < 2:847276/151‑1 (MQ=255) aaCGCGATATGGTACGGCGGGCAGGCTGCAGTAACGAGGGTA‑CGCCTTTTCTTGACGAGGGAGGTTTTTAAGTTGGCGGGGGTCAGCA‑GGGGTTTGGTTTCTTGGTAGAGATAAGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaaccc > 2:71137/1‑146 (MQ=255) cgATATGGTACGGCGGGCAGGCCGCAGGACCGAGGGGA‑CGCATTTTCTCGACGAGGGAGGTTTTTTGTTTGCCGGGGGGCAGCA‑GGGCCTTTGTTTTCTGGTGGAGATACGTTTTGTTGGCGGAGCCGCCGCCTTTCGCCACGCAAAgg > 1:934077/1‑149 (MQ=255) ggCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGcc < 2:654961/151‑1 (MQ=255) cAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt > 1:403685/1‑151 (MQ=255) ggCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCt < 2:412969/151‑1 (MQ=255) ggCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCt < 1:343144/151‑1 (MQ=255) aCCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTAcc < 1:679139/145‑1 (MQ=255) aCCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTAcc > 2:679202/1‑145 (MQ=255) aCCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTa < 1:877576/150‑1 (MQ=255) cGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAg < 1:451396/151‑1 (MQ=255) cGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAg < 1:675323/151‑1 (MQ=255) gAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAgg < 2:610571/151‑1 (MQ=255) gAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAgg < 2:19797/151‑1 (MQ=255) aGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAgg > 1:424809/1‑150 (MQ=255) gggTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTc < 2:742705/151‑1 (MQ=255) gggTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTc < 2:1083564/151‑1 (MQ=255) gggTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTc > 1:876166/1‑151 (MQ=255) | AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTA‑CGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTC > NC_000913/4346527‑4346822 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |