Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,538,109	0	A	C	47.6%	80.0 / 31.3	63	G219G (GGT→GGG)	nanM	N‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base A (27/6);  new base C (0/30);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 4.12e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTG  >  NC_000913/4537960‑4538253                                                                                                                                                         |                                                                                                                                                  aTCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCa                                                                                                                                                   <  1:824320/150‑1 (MQ=255) aTCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCa                                                                                                                                                   <  1:75417/150‑1 (MQ=255)  tCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCAcc                                                                                                                                                 >  1:468642/1‑151 (MQ=255)       gCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTAt                                                                                                                                            <  1:910796/151‑1 (MQ=255)         aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTAtt                                                                                                                                           <  2:480311/150‑1 (MQ=255)         aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTc                                                                                                                                          <  2:809389/151‑1 (MQ=255)              cGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAc                                                                                                                                    >  1:788206/1‑151 (MQ=255)                ccAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAccg                                                                                                                                  <  2:894692/151‑1 (MQ=255)                  aGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAccgcc                                                                                                                                <  2:89023/151‑1 (MQ=255)                  aGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAccgcc                                                                                                                                >  2:473705/1‑151 (MQ=255)                   gCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAccgccg                                                                                                                               >  2:775728/1‑151 (MQ=255)                       cGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCAcc                                                                                                                           >  1:870217/1‑151 (MQ=255)                          cATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGc                                                                                                                        >  2:628848/1‑151 (MQ=255)                            tCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCg                                                                                                                      >  1:64616/1‑151 (MQ=255)                                        aggggAGAAAGTTTATCCCATTTTAAATTATTCCCGGTAAAACAAATTTAAAATCCGGCACCCGTCCGCACCCCTGGTTTGGCTCCGCCATA‑AATAGG‑CCGGT‑TTTTACCCCCTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAg                                                                                                           <  1:216172/148‑1 (MQ=255)                                          gggggaatgtttATTCCATTTAAAATTATAACCGGAGAAATAAATTTAAAAAACGCCCCCCGTCCCAAATCCTGGTTTGCCTCCGCCATA‑AAAAGG‑CCGGT‑TTTTATCCCTTTTATCCAC‑ACCCGCCGCACCAGCCGTTCCGTACCAggg                                                                                                         <  1:54844/140‑1 (MQ=255)                                          gggAGAAAGTTTTTTCCTTTTAAATTTATTCCGGGTAAAATAAATTTAAAATACGGCACCCGTCCGCATCCCTGGTTTGCTTCCCCCATA‑AAAAAC‑CCAGT‑TTTTACCCCTTTTATCCAA‑ACCCGCCGCACCAGCCGTTCCGt                                                                                                                <  1:38264/143‑1 (MQ=255)                                              gCAAGCTTATTCCATTTTAAATTATTACCGGAGAAATCAAGTTCAAATACGGCATCCGTTCGAAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGAtt                                                                                                    >  2:931900/1‑151 (MQ=255)                                                aacttttttctttttaAAATTATTCCCGGTAAAACAAATTTAAAATCCGCCTCCCGTCCCCAATCCGTTTTTGTCTCCCCCTTA‑AAAACC‑CCAGT‑TTTTACCCCCTTTTTCAAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTc                                                                                                   <  1:1091566/138‑1 (MQ=255)                                                 aGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑ATCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                 >  2:736042/1‑151 (MQ=255)                                                  gCTTATTCCATTTTAAATTGTTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                >  2:809101/1‑151 (MQ=255)                                                    ttATTCCATTTTAAATTATTCCCGGTAAAATAAAGTTAAAATCCGGCATCCTTCCGCAATCCTGGTTTGGCTTCCCAATT‑AATAAG‑CCAGG‑TTTTACCCCTTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCa                                                                                               <  2:893311/150‑1 (MQ=255)                                                    ttATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                >  2:830661/1‑149 (MQ=255)                                                     tATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGc                                                                                             >  2:949687/1‑151 (MQ=255)                                                     tATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGc                                                                                             >  1:482713/1‑151 (MQ=255)                                                        tCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGt                                                                                           >  2:218176/1‑150 (MQ=255)                                                            tttaAATTTATTCCCGGTAAAATAAATTTAAAATCCGCCATCCGTCCGAAATCCTGTTTTGCCTCCCCCATT‑AATAAG‑CGGGG‑TTTAACCCCTTTAATCCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCg                                                                                            <  1:406266/145‑1 (MQ=255)                                                               tAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAt                                                                                   >  2:639051/1‑151 (MQ=255)                                                                aaaTTATTCCCGGTAAAATCAAGTTAAAATCCGGCTTCCGTTCGCAATCCGGGTTTGGCTTCGCCATA‑AATAAG‑CCAGG‑TTTTACCCCCTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAt                                                                                   <  2:169693/150‑1 (MQ=255)                                                                 aaTTATTCCCGTTAAAATAAATTTAAAATCCGGCACCCGTTCGCAATCCTGTTTTGTCTTCCCCATT‑AATAAG‑CCGGT‑TTTTACCCCCTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTg                                                                                 <  2:702305/151‑1 (MQ=255)                                                                   ttattaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCt                                                                               >  2:741083/1‑151 (MQ=255)                                                                               aaacaaaGTTCAAATACGGCTTCCTTTCCAAACCCGGGTTGGGTTTCCCAATA‑AAAAAG‑CCGGT‑TTTTATCACTTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAggg                                                                     <  2:629458/144‑1 (MQ=255)                                                                               aaaTAAAGTAAAAATCCGCCATCC‑TTCCCAATCCCTGTTTGGGCTCCCCCTT‑AAATAG‑CCCGGTTTTTATCCCTTTTTTCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAgg                                                                      <  1:473663/148‑1 (MQ=255)                                                                                    aatttaaaatcgggCATCCGTCCGCAATCCGGTTTTGGCTCCCCCATA‑AAAAAG‑CCAGT‑TTTTACCCCCTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaa                                                               <  2:1047975/138‑1 (MQ=255)                                                                                    aatttCAAATCCGCCACCCTTTC‑CCATTCTTGTTTGGGTTCCCCCAT‑AAAAAGCCCAGT‑TTTTATCCCTTTTATCCAC‑ACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaaa                                                              <  1:834053/148‑1 (MQ=255)                                                                                       tacAAATACGCCATCCGTTC‑CAAACCTTGTTTGGTTTCCCCATTAAAAAAG‑CCGGT‑TTTTATCCCTTTTACACAC‑ACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCa                                                                 <  1:332700/143‑1 (MQ=255)                                                                                         cAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGaca                                                          >  2:876547/1‑150 (MQ=255)                                                                                          aattccggaacccGTACACAATCCTGGTTTGGCTACCCCTTA‑AAAACC‑CCAGT‑TTTAACCCCCTTAATTCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGa                                                            <  2:140160/136‑1 (MQ=255)                                                                                          aaaTACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCAGTGTTGAGGGATCAAAAGacaac                                                        >  1:670208/1‑151 (MQ=255)                                                                                             tACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGacaac                                                        >  2:582600/1‑148 (MQ=255)                                                                                                ggCATCCTTTCGAAACCCTGGTTTGGTTCCGCAATA‑AAAAAC‑CCGGT‑TTTTACCCCCTTTATCCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGa                                                     <  2:149614/148‑1 (MQ=255)                                                                                                ggCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGAATCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAAc                                                  >  1:912286/1‑151 (MQ=255)                                                                                                     cttttCGCAATCCTGGTTTGGCTTCCCAATA‑AATAAG‑CCATG‑TTTTATCCCCTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                <  2:481710/145‑1 (MQ=255)                                                                                                     cctttCCCATTCCTGTTTTGGCTTCGCCATT‑AAAAAG‑CCAGT‑TTTTACCCCCTTTATTCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAt                                              <  2:320691/147‑1 (MQ=255)                                                                                                        ttCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTga                                           >  1:67684/1‑150 (MQ=255)                                                                                                        tccccAACCCGGGTTTGCTTCCCCCATT‑AATAAG‑CCAGG‑TTTTACCCCCTTTTTCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                               <  2:728123/142‑1 (MQ=255)                                                                                                        tcccaaaTCCGGTTTTGTCTTCCCCATA‑AATAAC‑CCAGT‑TTTTATCCCTTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                            <  2:287372/144‑1 (MQ=255)                                                                                                        tccGCAATCTGTGTTGGGCTCCGCCTTT‑AAAAAC‑CCGGT‑TTTTACCCCTTTTTTCCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt                                             <  2:1008920/146‑1 (MQ=255)                                                                                                           ccAATCTTGGTTTGCCTTCCCAATT‑AAAAAC‑CCAGT‑TTTTACCCCTTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaagaa                                       <  1:282234/150‑1 (MQ=255)                                                                                                             attCCTGTTTTGCCTCCCCCATA‑AAAAAC‑CCGGT‑TTTTACCCCTTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaagaa                                       <  1:931132/147‑1 (MQ=255)                                                                                                                 cTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtc                                 >  1:1105222/1‑151 (MQ=255)                                                                                                                   gttttGTCTTCGCCATT‑AA‑AAG‑ACCTG‑TTTTAACCTCCTTATCCCCAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaaga                                        <  1:471063/140‑1 (MQ=255)                                                                                                                   ggTTTGCTTTCCCCATT‑AATAAC‑CCAGT‑TTTTATCCCTTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtctt                               <  1:71318/151‑1 (MQ=255)                                                                                                                           ttCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                       >  1:56171/1‑151 (MQ=255)                                                                                                                           ttCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                       >  1:1114178/1‑151 (MQ=255)                                                                                                                               ccATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGt                   >  2:428491/1‑151 (MQ=255)                                                                                                                                 aTT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                  >  1:1085833/1‑150 (MQ=255)                                                                                                                                 aTT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                  >  1:1124841/1‑150 (MQ=255)                                                                                                                                   taaaaagg‑CCGGG‑TTTTACCCCCTTTATCCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGt                   <  2:547527/141‑1 (MQ=255)                                                                                                                                     aacaaG‑CAAGG‑TTTTACCCCTTTAACTCAC‑ACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaa                <  1:814260/146‑1 (MQ=255)                                                                                                                                      aaaaagcgggg‑TTTTACCCCTTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                  <  1:772638/138‑1 (MQ=255)                                                                                                                                       aaaG‑CCAGG‑TTTTATCCCCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATa            <  2:246178/150‑1 (MQ=255)                                                                                                                                            ccAGT‑TTTTACCCCCTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGt          <  2:125156/149‑1 (MQ=255)                                                                                                                                                  ttttACCCCCTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAt    <  1:76323/150‑1 (MQ=255)                                                                                                                                                    ttATCCCCTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTg  <  2:218447/150‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                  ATCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTG  >  NC_000913/4537960‑4538253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑