Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,538,117	0	T	C	42.6%	78.6 / 21.8	62	N217D (AAT→GAT)	nanM	N‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base T (27/8);  new base C (0/26);  total (27/35)
Fisher's exact test for biased strand distribution p-value = 1.63e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTAT  >  NC_000913/4537968‑4538264                                                                                                                                                         |                                                                                                                                                     aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTAtt                                                                                                                                                      <  2:480311/150‑1 (MQ=255) aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTc                                                                                                                                                     <  2:809389/151‑1 (MQ=255)      cGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAc                                                                                                                                               >  1:788206/1‑151 (MQ=255)        ccAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAccg                                                                                                                                             <  2:894692/151‑1 (MQ=255)          aGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAccgcc                                                                                                                                           <  2:89023/151‑1 (MQ=255)          aGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAccgcc                                                                                                                                           >  2:473705/1‑151 (MQ=255)           gCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AAccgccg                                                                                                                                          >  2:775728/1‑151 (MQ=255)               cGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCAcc                                                                                                                                      >  1:870217/1‑151 (MQ=255)                  cATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGc                                                                                                                                   >  2:628848/1‑151 (MQ=255)                    tCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCg                                                                                                                                 >  1:64616/1‑151 (MQ=255)                                aggggAGAAAGTTTATCCCATTTTAAATTATTCCCGGTAAAACAAATTTAAAATCCGGCACCCGTCCGCACCCCTGGTTTGGCTCCGCCATA‑AATAGG‑CCGGT‑TTTTACCCCCTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAg                                                                                                                      <  1:216172/148‑1 (MQ=255)                                  gggggaatgtttATTCCATTTAAAATTATAACCGGAGAAATAAATTTAAAAAACGCCCCCCGTCCCAAATCCTGGTTTGCCTCCGCCATA‑AAAAGG‑CCGGT‑TTTTATCCCTTTTATCCAC‑ACCCGCCGCACCAGCCGTTCCGTACCAggg                                                                                                                    <  1:54844/140‑1 (MQ=255)                                  gggAGAAAGTTTTTTCCTTTTAAATTTATTCCGGGTAAAATAAATTTAAAATACGGCACCCGTCCGCATCCCTGGTTTGCTTCCCCCATA‑AAAAAC‑CCAGT‑TTTTACCCCTTTTATCCAA‑ACCCGCCGCACCAGCCGTTCCGt                                                                                                                           <  1:38264/143‑1 (MQ=255)                                      gCAAGCTTATTCCATTTTAAATTATTACCGGAGAAATCAAGTTCAAATACGGCATCCGTTCGAAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGAtt                                                                                                               >  2:931900/1‑151 (MQ=255)                                        aacttttttctttttaAAATTATTCCCGGTAAAACAAATTTAAAATCCGCCTCCCGTCCCCAATCCGTTTTTGTCTCCCCCTTA‑AAAACC‑CCAGT‑TTTTACCCCCTTTTTCAAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTc                                                                                                              <  1:1091566/138‑1 (MQ=255)                                         aGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑ATCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                            >  2:736042/1‑151 (MQ=255)                                          gCTTATTCCATTTTAAATTGTTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                           >  2:809101/1‑151 (MQ=255)                                            ttATTCCATTTTAAATTATTCCCGGTAAAATAAAGTTAAAATCCGGCATCCTTCCGCAATCCTGGTTTGGCTTCCCAATT‑AATAAG‑CCAGG‑TTTTACCCCTTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCa                                                                                                          <  2:893311/150‑1 (MQ=255)                                            ttATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                           >  2:830661/1‑149 (MQ=255)                                             tATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGc                                                                                                        >  2:949687/1‑151 (MQ=255)                                             tATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGc                                                                                                        >  1:482713/1‑151 (MQ=255)                                                tCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGt                                                                                                      >  2:218176/1‑150 (MQ=255)                                                    tttaAATTTATTCCCGGTAAAATAAATTTAAAATCCGCCATCCGTCCGAAATCCTGTTTTGCCTCCCCCATT‑AATAAG‑CGGGG‑TTTAACCCCTTTAATCCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCg                                                                                                       <  1:406266/145‑1 (MQ=255)                                                       tAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAt                                                                                              >  2:639051/1‑151 (MQ=255)                                                        aaaTTATTCCCGGTAAAATCAAGTTAAAATCCGGCTTCCGTTCGCAATCCGGGTTTGGCTTCGCCATA‑AATAAG‑CCAGG‑TTTTACCCCCTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAt                                                                                              <  2:169693/150‑1 (MQ=255)                                                         aaTTATTCCCGTTAAAATAAATTTAAAATCCGGCACCCGTTCGCAATCCTGTTTTGTCTTCCCCATT‑AATAAG‑CCGGT‑TTTTACCCCCTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTg                                                                                            <  2:702305/151‑1 (MQ=255)                                                           ttattaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCt                                                                                          >  2:741083/1‑151 (MQ=255)                                                                       aaacaaaGTTCAAATACGGCTTCCTTTCCAAACCCGGGTTGGGTTTCCCAATA‑AAAAAG‑CCGGT‑TTTTATCACTTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAggg                                                                                <  2:629458/144‑1 (MQ=255)                                                                       aaaTAAAGTAAAAATCCGCCATCC‑TTCCCAATCCCTGTTTGGGCTCCCCCTT‑AAATAG‑CCCGGTTTTTATCCCTTTTTTCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAgg                                                                                 <  1:473663/148‑1 (MQ=255)                                                                            aatttaaaatcgggCATCCGTCCGCAATCCGGTTTTGGCTCCCCCATA‑AAAAAG‑CCAGT‑TTTTACCCCCTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaa                                                                          <  2:1047975/138‑1 (MQ=255)                                                                            aatttCAAATCCGCCACCCTTTC‑CCATTCTTGTTTGGGTTCCCCCAT‑AAAAAGCCCAGT‑TTTTATCCCTTTTATCCAC‑ACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaaa                                                                         <  1:834053/148‑1 (MQ=255)                                                                               tacAAATACGCCATCCGTTC‑CAAACCTTGTTTGGTTTCCCCATTAAAAAAG‑CCGGT‑TTTTATCCCTTTTACACAC‑ACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCa                                                                            <  1:332700/143‑1 (MQ=255)                                                                                 cAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGaca                                                                     >  2:876547/1‑150 (MQ=255)                                                                                  aattccggaacccGTACACAATCCTGGTTTGGCTACCCCTTA‑AAAACC‑CCAGT‑TTTAACCCCCTTAATTCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGa                                                                       <  2:140160/136‑1 (MQ=255)                                                                                  aaaTACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCAGTGTTGAGGGATCAAAAGacaac                                                                   >  1:670208/1‑151 (MQ=255)                                                                                     tACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGacaac                                                                   >  2:582600/1‑148 (MQ=255)                                                                                        ggCATCCTTTCGAAACCCTGGTTTGGTTCCGCAATA‑AAAAAC‑CCGGT‑TTTTACCCCCTTTATCCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGa                                                                <  2:149614/148‑1 (MQ=255)                                                                                        ggCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGAATCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAAc                                                             >  1:912286/1‑151 (MQ=255)                                                                                             cttttCGCAATCCTGGTTTGGCTTCCCAATA‑AATAAG‑CCATG‑TTTTATCCCCTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                           <  2:481710/145‑1 (MQ=255)                                                                                             cctttCCCATTCCTGTTTTGGCTTCGCCATT‑AAAAAG‑CCAGT‑TTTTACCCCCTTTATTCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAt                                                         <  2:320691/147‑1 (MQ=255)                                                                                                ttCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTga                                                      >  1:67684/1‑150 (MQ=255)                                                                                                tccccAACCCGGGTTTGCTTCCCCCATT‑AATAAG‑CCAGG‑TTTTACCCCCTTTTTCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                          <  2:728123/142‑1 (MQ=255)                                                                                                tcccaaaTCCGGTTTTGTCTTCCCCATA‑AATAAC‑CCAGT‑TTTTATCCCTTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                                       <  2:287372/144‑1 (MQ=255)                                                                                                tccGCAATCTGTGTTGGGCTCCGCCTTT‑AAAAAC‑CCGGT‑TTTTACCCCTTTTTTCCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt                                                        <  2:1008920/146‑1 (MQ=255)                                                                                                   ccAATCTTGGTTTGCCTTCCCAATT‑AAAAAC‑CCAGT‑TTTTACCCCTTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaagaa                                                  <  1:282234/150‑1 (MQ=255)                                                                                                     attCCTGTTTTGCCTCCCCCATA‑AAAAAC‑CCGGT‑TTTTACCCCTTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaagaa                                                  <  1:931132/147‑1 (MQ=255)                                                                                                         cTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtc                                            >  1:1105222/1‑151 (MQ=255)                                                                                                           gttttGTCTTCGCCATT‑AA‑AAG‑ACCTG‑TTTTAACCTCCTTATCCCCAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaaga                                                   <  1:471063/140‑1 (MQ=255)                                                                                                           ggTTTGCTTTCCCCATT‑AATAAC‑CCAGT‑TTTTATCCCTTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtctt                                          <  1:71318/151‑1 (MQ=255)                                                                                                                   ttCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                                  >  1:56171/1‑151 (MQ=255)                                                                                                                   ttCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                                  >  1:1114178/1‑151 (MQ=255)                                                                                                                       ccATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGt                              >  2:428491/1‑151 (MQ=255)                                                                                                                         aTT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                             >  1:1124841/1‑150 (MQ=255)                                                                                                                         aTT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                             >  1:1085833/1‑150 (MQ=255)                                                                                                                           taaaaagg‑CCGGG‑TTTTACCCCCTTTATCCCC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGt                              <  2:547527/141‑1 (MQ=255)                                                                                                                             aacaaG‑CAAGG‑TTTTACCCCTTTAACTCAC‑ACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaa                           <  1:814260/146‑1 (MQ=255)                                                                                                                              aaaaagcgggg‑TTTTACCCCTTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                             <  1:772638/138‑1 (MQ=255)                                                                                                                               aaaG‑CCAGG‑TTTTATCCCCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATa                       <  2:246178/150‑1 (MQ=255)                                                                                                                                    ccAGT‑TTTTACCCCCTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGt                     <  2:125156/149‑1 (MQ=255)                                                                                                                                          ttttACCCCCTTTATCCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAt               <  1:76323/150‑1 (MQ=255)                                                                                                                                            ttATCCCCTTTATCCAA‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTg             <  2:218447/150‑1 (MQ=255)                                                                                                                                                 ctcTTTATCCCC‑ACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGa            <  2:450392/144‑1 (MQ=255)                                                                                                                                                       aTTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCtat  >  1:132532/1‑150 (MQ=255)                                                                                                                                                         |                                                                                                                                                     AAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAG‑CCAGG‑TTTTATCACCTTTATTCAC‑AACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTAT  >  NC_000913/4537968‑4538264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑