| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,880 | 0 | T | C | 44.0% | 57.7 / 20.0 | 50 | K271E (AAA→GAA) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base T (19/9); new base C (0/22); total (19/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.34e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGT > NC_000913/357733‑358026 | cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTcc < 2:42181/150‑1 (MQ=255) gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTccgc > 1:501254/1‑151 (MQ=255) gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTccgc > 1:272748/1‑151 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTccgccg < 2:594949/151‑1 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTccgcc < 2:1017791/150‑1 (MQ=255) ggCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCcgccgc > 2:1094901/1‑151 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGc‑aac > 1:518230/1‑150 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCCGCCCTGTGCCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACaaa < 1:277835/151‑1 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACaaaa < 2:17119/150‑1 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAAc > 1:123078/1‑151 (MQ=255) cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTtc > 2:1051340/1‑151 (MQ=255) aTCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTtctc > 2:911628/1‑150 (MQ=255) aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTcc > 1:1062720/1‑150 (MQ=255) ccAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGc > 1:358491/1‑151 (MQ=255) ccAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGc > 1:66079/1‑151 (MQ=255) agccttgtgTCAGCGGCTCCCGTGATTCATTCCCGCCAA‑CACCCGCGCTTTTAACAAACCCTTTGTGAAAAAAT‑GCTTT‑CGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCAA‑AAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGG‑Caa < 2:1081186/145‑1 (MQ=255) gACCTGTGCCAGCGCCTCCCGTGATTCATTCCCGCAAA‑CACCCGCGCATTTACCCACCGCAAGGTGAAAAAAG‑GCCTC‑CGGGGCGGCTGTCCGCCAGTTTTTTTCCCGCCGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑Ca < 2:26073/148‑1 (MQ=255) acttttgtCAGCGCCTCCCGTGTTTCTTTCCCCCCAA‑CACCCGCGCTTTTACCCACCGCCATGTGAAAAAAG‑GCTTC‑CGGGGCGGCTGTCTGCCATTTTTTTTTCCGCCCC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCgggg < 2:391629/140‑1 (MQ=255) ccTGTGTCAGCGGCTACCGTGATTCTTTCCCCCCAA‑CACCCGCGCATTTACCCACCCCCTTGTAAAAAAAT‑CCCTC‑CGGGGCGGCTGTCTCCCACCTTTTTTCCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAga‑g‑a < 2:268722/151‑1 (MQ=21) cTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCggagggt > 1:997022/1‑141 (MQ=255) tgtgTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑AAaga‑gga > 2:309451/1‑148 (MQ=255) gtCGGCGGCTCCCGTAATTCTTCCCCCCAAA‑CACCCCCGATTTTACCCACCCCCATG‑GAAAAAAT‑TCCTTCCGGGGCGGCTGTCTCCCGGTTTTTTTCCCGCCAA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑aa < 1:469523/147‑1 (MQ=255) cggCGGCTCCCGTTATTCATCCCCGCCAA‑CCCCCGCGCTTTTCCCCACCGCCATG‑GAAAAAATGGCCTC‑CGCGGGGGCTGCCGCCCAGTTATTTTCCCGCCAA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAg < 1:385346/147‑1 (MQ=255) gCGGCTACGGTAATTCTTTCCCGCAAA‑CACCCCCGTTTTAACCCAACCCCATGTCAAAAAAT‑GCCTT‑CGGGGCGGCTGTCGGCCTGTTTTTTTCCCGCCGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATa < 2:939751/146‑1 (MQ=255) aCCGTGATTCATTCCCCCCAA‑CACCCCCGCTTTAACCCACCGCGGGGTGAAAAAAT‑CCCTA‑GGCGGCGCTCGTCTGCCAGCTTTTTCCCCGCCGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTa < 2:505210/146‑1 (MQ=255) ccGTAATTCTTTCCCCCAAA‑CACCCCCGCTTTTCCCCAACCCCATGTGAAAAAAG‑GCTTT‑GGCGGCGGCTGCCGGCAGGCTTTTTTCCCGCGGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCttt < 1:576002/144‑1 (MQ=255) taattctttCCCCCAACCACCCCCGCTTTTACCCCCCGCCAGGTAAAAAAAC‑CCCTC‑CGGGGCGCTTGTC‑GCCCGTTTTTTTTCCCCCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGcc < 1:792276/140‑1 (MQ=255) ttCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CGAGA‑G‑GAAAAAGTTTAAAccccccctct > 2:456940/1‑144 (MQ=25) cATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCggcgggagaggga > 1:187500/1‑118 (MQ=255) ttcccccaaa‑caccCGCCCTTTTACCCAACGCCATGGAAAAAAAT‑CCCTC‑CGCGGCGGTTGTCTCCCAGTTTTTTTCCCGCCAA‑AAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCAt < 1:197719/137‑1 (MQ=255) cGCCAC‑CACCCGCGCTTTTAGCCAACGCGATTGGAAAAACG‑GCCTT‑CGCGGCGGCTGTCTCCCATTTATTTTCCCCCCCA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCAtgtt < 2:1047781/144‑1 (MQ=255) ccAA‑CACCCGCGCTTTTACCCACCCCCATGTGAAAAAAT‑GCCTC‑CGGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCa < 2:368693/150‑1 (MQ=255) aa‑caccCGCGCATTTACCCAACGCCTTGTGAAAAAAT‑GCCTT‑CGGGGCGGCTTTCTGCCAGCTTTTTTCCCGCCGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATc < 2:209819/151‑1 (MQ=255) ccGCGTATTTACCCACCCCCTTGTAAAAAAGT‑CCCCC‑CGCGGCGGCTCCCTGCCAGTTTTTTTCCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgct < 1:1051245/149‑1 (MQ=255) tacccAACGCCATGTGCAAAATT‑GCCTC‑CGCGGCGGCTGTCTGCCAGTTTTTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACa < 2:467886/147‑1 (MQ=255) ccACCGCCAGGTGAAAAAAT‑CCCTT‑CGGGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtg < 2:501296/150‑1 (MQ=255) acccccttgtgAAAAAAT‑GCCTT‑CGGGGCGGCTGTCTGCCAGTTATTTTCCCGCCGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtgtg < 2:781045/143‑1 (MQ=255) ccATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑GGAGAGG‑AAAAAAATTTAA‑CCCCCCCTGTTTTGTGGGGATCGCTGCTggggaaaaaatgg > 1:1015016/1‑136 (MQ=21) tgCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑GAAGA‑G‑AAAAAAAGTTAAATCCGCCCAGTTTTGTGGGAAACGCCGTTGGGGACAATGAGGAAAggg > 2:312261/1‑148 (MQ=255) aaaaaC‑GCCTC‑CGGGGGGGCTGTCGCCAATCTTTTTTCCCCCCGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGa < 1:500222/146‑1 (MQ=255) aaT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAACG‑G‑AAAAACGTTTAATCCC‑TCCCGTTGTGTGGGGAACGCTGCTTGTGACAATGTGGGAAGGGGAgagggg > 2:625971/1‑145 (MQ=255) aacgctctggcggcgGCTGCCGGCCAGTTTTTTCCCCGCCAC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggc < 1:363883/142‑1 (MQ=255) tcggcggggGCTGTCTCCCGGTTTTTTTCCCGCCAC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGaa < 1:434163/145‑1 (MQ=255) gggggggcTGCCGGCCATTTTTTTTCCCGCCGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa < 1:524112/143‑1 (MQ=255) ggcggcTGTCTGCCATCTTTTTTCCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa < 1:873583/146‑1 (MQ=255) gcTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACAGCCGTTCTCTCCAGTAGTGGCGGGG‑CGAGA‑G‑AAAAAAATTTTAACCCCCCCTGTTTTGTGGGGATCGCTGCTGGTTACAAAATGGGAAGGGGAGGGGGGGGGATCaaatcc > 2:489899/1‑142 (MQ=255) cTGCCGGCTTTTTTTCCGCCGA‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc < 1:994824/147‑1 (MQ=255) tgcaagtttttttcCCGCCGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggcg < 2:912005/122‑1 (MQ=255) gCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑GAAAAAAACTTTAAACCC‑CCCTGTTGTGTGGGGAGTGCTTCTGGGAACAATGTGGGGAGGGGAGGGGGGGGGAGCAACTCCCAAAACgcgc > 2:782822/1‑150 (MQ=255) ccAGTTTTTTTCCCGCCAA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAcc < 1:728133/145‑1 (MQ=255) acttATTTTCCCCCCGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTa < 2:384274/146‑1 (MQ=255) tttttcCCGCCGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTaa < 2:262791/145‑1 (MQ=255) tttttCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑GAAAAAAAATTTAAACCC‑CCCTGTTGTTTGGGGATCCCCGCCGGGGACAAAGTGGGAAGGGGAGGGCGGGGGATCAGCTCCACAACCGCCCCAAtt > 2:1003184/1‑147 (MQ=255) ttttCCGCCGA‑AACAAATCCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGt < 2:76670/150‑1 (MQ=255) | CGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGG‑CAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGT > NC_000913/357733‑358026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |