Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,810,459	T→C	V39A (GTA→GCA)	ygaH →	putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,459	0	T	C	100.0%	220.1 / NA	57	V39A (GTA→GCA)	ygaH	putative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/23);  total (34/23)

CGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAAC  >  NC_000913/2810311‑2810603                                                                                                                                                     |                                                                                                                                                 cGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggcag                                                                                                                                                <  2:799098/151‑1 (MQ=255)  gTTAATCCAGGCATTCTGGCACGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGACTTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggcagg                                                                                                                                               >  1:424881/1‑151 (MQ=255)   ttAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCATGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggcagg                                                                                                                                               >  1:84562/1‑150 (MQ=255)    tAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggcaggt                                                                                                                                              <  1:985399/150‑1 (MQ=255)       tCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTAttt                                                                                                                                          >  1:104522/1‑151 (MQ=255)         cAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTg                                                                                                                                        <  1:885768/151‑1 (MQ=255)           ggCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCt                                                                                                                                      <  1:837844/151‑1 (MQ=255)              aTTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAACCGTGGCGCGGCAGGTATTTTGCTCg                                                                                                                                    >  1:220412/1‑150 (MQ=255)              aTTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCg                                                                                                                                    >  2:487514/1‑150 (MQ=255)                   ggCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGaca                                                                                                                                 <  2:714348/148‑1 (MQ=255)                           gcgcCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATc                                                                                                                      <  1:1047419/151‑1 (MQ=255)                            cgcCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCg                                                                                                                     <  1:328260/151‑1 (MQ=255)                            cgcCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCg                                                                                                                     >  2:427876/1‑151 (MQ=255)                              cccGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGcc                                                                                                                   >  1:583138/1‑151 (MQ=255)                               ccGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCt                                                                                                                  >  2:456967/1‑151 (MQ=255)                               ccGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCt                                                                                                                  <  2:879317/151‑1 (MQ=255)                                  aTGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCg                                                                                                                >  1:589881/1‑150 (MQ=255)                                    gAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGat                                                                                                              >  2:563029/1‑150 (MQ=255)                                    gAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGat                                                                                                              <  2:993891/150‑1 (MQ=255)                                        tATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATgcg                                                                                                         >  2:21439/1‑151 (MQ=255)                                         aTGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATgcg                                                                                                         <  2:58906/150‑1 (MQ=255)                                          tGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATgc                                                                                                          >  1:999486/1‑148 (MQ=255)                                           gAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGct                                                                                                       <  2:963329/150‑1 (MQ=255)                                              gTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTctgc                                                                                                   >  1:856140/1‑151 (MQ=255)                                                tCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTctgctg                                                                                                 >  1:554972/1‑151 (MQ=255)                                                  tgctgcTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCCCGGCAGGTATTTTGCTCGACACCATTGGCATCACCCCCATATGCGCTccgctg                                                                                                 >  1:479801/1‑149 (MQ=255)                                                     tgctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGt                                                                                            >  1:84259/1‑151 (MQ=255)                                                       ctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGtct                                                                                          >  2:1107555/1‑151 (MQ=255)                                                          gggTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTctct                                                                                        >  2:1024568/1‑150 (MQ=255)                                                            gTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAcc                                                                                     >  1:570356/1‑151 (MQ=255)                                                             ttACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAcc                                                                                     >  1:33233/1‑150 (MQ=255)                                                               aCTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGc                                                                                   >  1:215747/1‑150 (MQ=255)                                                               aCTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGGATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGc                                                                                   >  1:966998/1‑150 (MQ=255)                                                                cTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCAc                                                                                 >  1:419772/1‑151 (MQ=255)                                                                          tggcgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGAt                                                                       >  1:178781/1‑151 (MQ=255)                                                                             cgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGc                                                                     >  2:125745/1‑150 (MQ=255)                                                                             cgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCa                                                                    <  1:226369/151‑1 (MQ=255)                                                                                attattGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACg                                                                  >  2:1104746/1‑150 (MQ=255)                                                                                   attGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATac                                                              >  1:1065751/1‑151 (MQ=255)                                                                                     tGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATacac                                                            >  1:662145/1‑151 (MQ=255)                                                                                     tGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATaca                                                             <  1:993572/150‑1 (MQ=255)                                                                                         tCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGcc                                                         <  1:592952/150‑1 (MQ=255)                                                                                         tCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGcc                                                         <  1:218241/150‑1 (MQ=255)                                                                                         tCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCg                                                        >  2:989582/1‑151 (MQ=255)                                                                                            gCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGttt                                                     >  1:863996/1‑151 (MQ=255)                                                                                                tttGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGt                                                  <  1:49013/150‑1 (MQ=255)                                                                                                   gCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGccc                                              <  2:447984/151‑1 (MQ=255)                                                                                                   gCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGccc                                              >  1:5962/1‑151 (MQ=255)                                                                                                      gCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGTTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACg                                           <  2:963465/151‑1 (MQ=255)                                                                                                         gcgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCCCCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTg                                        <  1:325989/151‑1 (MQ=255)                                                                                                                 gtgtGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCtt                                <  2:522231/151‑1 (MQ=255)                                                                                                                 gtgtGGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCtt                                >  1:430626/1‑151 (MQ=255)                                                                                                                   gtgGGTAATGCCCGCCCAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTcg                              <  2:508548/151‑1 (MQ=255)                                                                                                                            gcccgcccAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTgg                     >  2:382945/1‑151 (MQ=255)                                                                                                                              ccgcccAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGt                   <  1:1099637/151‑1 (MQ=255)                                                                                                                              ccgcccAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGt                   <  1:695555/151‑1 (MQ=255)                                                                                                                                 cccAACCAAACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGcc                >  2:405079/1‑151 (MQ=255)                                                                                                                                       caaACGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTc          <  1:727444/151‑1 (MQ=255)                                                                                                                                        aaaCGTGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCt         >  1:859218/1‑151 (MQ=255)                                                                                                                                             tGGCGCGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATaa     >  1:832605/1‑150 (MQ=255)                                                                                                                                               gcgcGGCAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAac  <  1:187658/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAAC  >  NC_000913/2810311‑2810603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑