Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,6920CA41.8% 84.3 / 27.6 55intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (2/30);  new base A (23/0);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.61e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

AAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903550‑2903841
                                                                                                                                              |                                                                                                                                                         
aaGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCAtt                                                                                                                                                   <  1:355315/151‑1 (MQ=255)
 aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTACAACCACTCATTa                                                                                                                                                  <  1:218335/151‑1 (MQ=255)
          aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑aaa                                                                                                                                        <  1:28700/150‑1 (MQ=255)
             tACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACACTCCTTATCCT‑C‑aaaa                                                                                                                                       >  1:502560/1‑148 (MQ=255)
                 cTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACCCATTATACT‑C‑CAaaaaaaaa                                                                                                                                 >  2:107049/1‑148 (MQ=255)
                     aTGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAAc                                                                                                                           <  1:378833/151‑1 (MQ=255)
                         cTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTTAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACTCATTAGCCT‑C‑CAaaaaaaaaa‑aaa                                                                                                                            >  2:257595/1‑146 (MQ=255)
                                       aCAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTa                                                                                                         <  1:313251/151‑1 (MQ=255)
                                         aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTTAAAAACACATTATACT‑C‑AAAACAAAAAA‑AAAATTCCAGATTTATTAttt                                                                                                          >  1:47317/1‑148 (MQ=255)
                                            aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCa                                                                                                     <  2:859027/150‑1 (MQ=255)
                                            aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCa                                                                                                     <  2:134133/150‑1 (MQ=255)
                                               aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTAACCT‑C‑AAAAAAAAAAA‑AAAATTTCAGATTTTTTACTTAAACAATg                                                                                                  >  2:152286/1‑150 (MQ=255)
                                                aTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGaa                                                                                                <  1:474039/151‑1 (MQ=255)
                                                  cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATa                                                                                              <  2:356949/151‑1 (MQ=255)
                                                       tttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAAATCATTTACCC‑C‑ACAAAAAAAAA‑AAAAATCCAGATTTATTATTTAACACATGAATAaaaa                                                                                          >  1:720743/1‑150 (MQ=255)
                                                            cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGTTTGTGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAATC‑TTa                                                                                    <  1:868208/150‑1 (MQ=255)
                                                            cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTa                                                                                    <  2:412437/150‑1 (MQ=255)
                                                              aTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTaaaa                                                                                 <  1:338402/151‑1 (MQ=255)
                                                                  tCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACTCATTATCCT‑C‑CAAAAAAAAAC‑AAAATTCCAGATTTTTTTCTTAAACAAAGAATAAAAAAC‑TTaaaaaa                                                                               >  2:516768/1‑149 (MQ=255)
                                                                   cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACACCCCTTAACCT‑C‑CAAAAAAAAAA‑AAAATTCCAGATTTTTTTCTTTAACAAATAATAACAAAC‑TTaaaaaaaa                                                                             >  2:601152/1‑148 (MQ=255)
                                                                     cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAAAACTCATTTACCTCC‑AAAAAAAAAAA‑AAAATTCCA‑ATTTTTTTATTTACCAAAAAAAAAAAAAC‑ATaaaaaaa                                                                              >  2:321265/1‑146 (MQ=255)
                                                                       tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTATAAAAACAATCCCTTCTCT‑CAAAAAAAAAAAA‑AAACTTCC‑GATTTTTTTCTTTACAAAAAAAAAAAAAACATTaaaaaa                                                                               >  2:323973/1‑145 (MQ=255)
                                                                       tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACt                                                                         <  1:453594/150‑1 (MQ=255)
                                                                           gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACAATCATCTTCCT‑CAAAAAAAAAAAA‑AAAATTCCGAATTTTTTA‑TTTACCAAAAAAAAAAAAGA‑TTaaaaaaaaaaatttttt                                                                    >  1:1184/1‑140 (MQ=255)
                                                                           gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTAt                                                                    <  2:835449/151‑1 (MQ=255)
                                                                              tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTTAAAACACACCTTATCCT‑C‑AAAAAAAAAAA‑AAAAATTCAGAATTTTTTTTTTAACAAAGAAAAAAAAAA‑CTAAAAAAAATAATGTTAtttt                                                                 >  2:421879/1‑149 (MQ=255)
                                                                                    ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACTCCTTATACT‑C‑AAAAAAAAAAA‑AAAATTCCAAATTTATTACTTAAAAAAAAAATAAAAAAC‑ATAAAAAAAATAATTTTTTTTTaaa                                                              >  1:395301/1‑148 (MQ=255)
                                                                                      atatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAACTCATTATCCT‑C‑AAAAAAAAGAC‑AAAATTCCAGATTTTTTTCTTAAACAAAGAATAAAAAAA‑TTAAAAAAAAAAATTTTATTTTTaaaaaa                                                          >  1:402495/1‑150 (MQ=255)
                                                                                       tatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACACCTTAAGCT‑C‑AAAAAAAAAACAAAACTTCC‑GATTTTTTTTTTAAACAAAGAAAAAAAAAA‑CTAAAAAAAATAATTTTTTTTTaaaaaaaa                                                         >  2:123775/1‑150 (MQ=255)
                                                                                        atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACCCATTATCCC‑C‑CAAAAAAAAAA‑AAAAATCCAGATTTATTTTTTTAACAAAAAAAAAAAAAA‑TTTAAAAAAAAAATGTTTTTTTAaaaaaaaa                                                        >  2:410083/1‑150 (MQ=255)
                                                                                        atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGaaaaa                                                        <  2:695688/150‑1 (MQ=255)
                                                                                        atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGaaaaa                                                        <  2:446365/150‑1 (MQ=255)
                                                                                           tttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACACTCATTAACCT‑C‑AAAAAAAAAAA‑AAAAATCCAGATTTATTTATTTAACAAAAAAAAAAAAAC‑TTAAAAAAAATAATTTTATTTTTAaaaaaaaaa                                                      >  2:262241/1‑148 (MQ=255)
                                                                                            ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACAACCATTAGCCT‑C‑AAAAAAAAAAA‑AAACTTCCAGATTTTTTTCTTTAACAAAAAAAAAAAAAC‑ATAAAAAAAATAATGTTATTTTaaaaaaaaaaa                                                      >  2:558592/1‑147 (MQ=255)
                                                                                            ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAAt                                                      <  2:811990/148‑1 (MQ=255)
                                                                                             tATTCATAAGAACTATTCATCAACAGAGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTTCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATCTACTGTTATTCTAAAGaaaaaa                                                       <  1:86825/146‑1 (MQ=255)
                                                                                              aTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTACCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAAc                                                  <  1:688330/150‑1 (MQ=255)
                                                                                               ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACTCATTAACCT‑C‑CAAAAAAAAAC‑AAAACTTCAGATTTTTTAATTAAACAAAGAAAAAAAAAC‑TTAAAAAAAATAATTTTATTTTAAAAAAAAAAATaa                                                   >  2:252352/1‑148 (MQ=255)
                                                                                                tCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAg                                                <  1:407665/150‑1 (MQ=255)
                                                                                                  aTAAGAACTATTCATCAACAGCGTATAGCGGCGGTGATATAAAAAAACTCTTTAATCT‑C‑TAAATAAAAAA‑AAAGATTAAGAATTATTATTTTATCAATTAAAAAAAAAA‑CTACAAAAAAAAAAGTTTTTTTaaaaaaaaaaattactgg                                               >  1:504833/1‑141 (MQ=255)
                                                                                                    aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAtt                                           <  1:279184/151‑1 (MQ=255)
                                                                                                       aaCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAt                                        <  1:478253/151‑1 (MQ=255)
                                                                                                        aCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAtt                                       <  1:733641/151‑1 (MQ=255)
                                                                                                          tATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTTACCT‑C‑AAAAAAAAAAA‑AAAATTTCCAATTTATTTCTTAAACAAAAAAAAAAAAAC‑TTAAAAAAAATAATTTTTTTTTAAAAAAAAAAATAAAAGGCAATAAtt                                       >  2:765087/1‑149 (MQ=255)
                                                                                                           aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTc                                      <  2:432295/149‑1 (MQ=255)
                                                                                                           aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTc                                      <  2:68254/149‑1 (MQ=255)
                                                                                                           aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAACATACTGTTAATCTAAAGAAAAAATTAACAGGCATTAATTCa                                     <  1:820899/150‑1 (MQ=255)
                                                                                                            ttCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCaa                                    <  2:502689/150‑1 (MQ=255)
                                                                                                             tcatcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAat                                   <  2:747927/150‑1 (MQ=255)
                                                                                                               atcaACAGCGTATAGAGGCGGTTTTGTAAAAACACTAATTATCCT‑C‑CAAAAAAAAAC‑AAAAATCCATAATTTTTATTTATACAAAAAAAAAAAAAA‑ATAAAAAAAATAATTTTTTTTTTAAAAAAAAAATAAAAAAAATTAATTTaaaa                                  >  1:827419/1‑147 (MQ=255)
                                                                                                                     aGCGTATAGAGGCGGTTATGTAAAAACACCCATTAGACT‑C‑AAAAAAAAAAA‑AAACTTCCAGATTTTTTACTTTAACAAAGAAAAAAAAAC‑TTAAAAAAAATACTTTTATTTTTAAGAAAAAAATAAAAAGCATTAATTTAATATTtaaaa                           >  2:302302/1‑150 (MQ=255)
                                                                                                                       cGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaaaa                         <  1:513659/150‑1 (MQ=255)
                                                                                                                             gagGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataat                     >  1:361590/1‑148 (MQ=255)
                                                                                                                                            aaCCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc   <  2:718949/151‑1 (MQ=255)
                                                                                                                                            aaCCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc   >  1:83705/1‑151 (MQ=255)
                                                                                                                                              ccACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACg  >  2:485679/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                         
AAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCT‑C‑AAAACAAAGAC‑AAACTTCCAGATTTATTACTTAACCAATGAATAACAAGC‑TTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903550‑2903841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑