Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,056,115 | 0 | A | C | 42.6% | 81.1 / 18.1 | 55 | G75G (GGT→GGG) | glnL | sensory histidine kinase in two‑component regulatory system with GlnG |
Reads supporting (aligned to +/- strand): ref base A (24/7); new base C (0/23); total (24/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.69e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGG > NC_000913/4055968‑4056262 | gTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCt < 2:590668/151‑1 (MQ=255) gCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTg < 1:196109/151‑1 (MQ=255) gCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTg < 2:771416/151‑1 (MQ=255) gTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑ccc > 1:174441/1‑151 (MQ=255) aGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCg < 2:834140/151‑1 (MQ=255) tCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTcc > 1:419617/1‑150 (MQ=255) ccggACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCGGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGCCCAGCGTCACTTCGTTATCGGAAAACCCTTG‑CCCCGCCTCCa < 1:287832/147‑1 (MQ=255) gcggcgCTGGTTCTCCATCGGACCCACCCCCAGCAGGATCTGCCCGCCCGTCATGCGCGGGGCCGTCACAAAAAGGATATGCGAGCGCCCGCCGATGACCAGCGTCACTTCGTTAGCGAAAAACCCTGG‑CCCCGCCTCCAGACTTTCTTGc < 1:526583/151‑1 (MQ=255) ggcgCTGGTTATCCACCGGAGCCTTCCCCAGCAGGATCATGCCGTCCGGCATACGCGGGGCCGTCACAGAAAGGATATGCGAGCCCCCGTCGATGACCAGCGTCACTTCTTTATCGGTAAACCCTTG‑CCCCGCCTCCAGACTTTCTTg < 2:702198/148‑1 (MQ=255) ggTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCCTCGATGACCAGCGGCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTc > 1:878587/1‑151 (MQ=255) ttATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCg > 1:709687/1‑150 (MQ=255) ttATCCATAGAGCCCACCTCCACCAGGATATGGCCCTCCGAAGTACGCTGGTCCTTCACAGAAAGCATATGCCGGCGCCCCTCGATCCCAAGCTTTCTTTCGTAAGCGTAAAATCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTc < 1:344535/149‑1 (MQ=255) ttATCCACCGGAGCCACCTCCAGCAGGATCATGCCGTCCGGCATCCGCGGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCATTTCGTTATCGGTAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCg < 1:594792/150‑1 (MQ=255) ccATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATAtt > 2:8345/1‑151 (MQ=255) ccATCGGACCCATCTCCAGCAGGATCATGCCGTCCGGCATCCGCGGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGAAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATAtt < 2:531045/151‑1 (MQ=255) agcGGGGCCATCCCAAGCGGTTTCACGCCGTCCGCCATCCGCTGGGCCGACACAAAAAGGATATGCGAGCGCCCATCGAGGACCAGCTTCACTTCTTTATCGGTAAACCCTTC‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTa < 2:711255/149‑1 (MQ=255) ggAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCGGGGCCGTCACAAAAAGGATATGCGAGCGCCCTTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTaa < 2:712142/149‑1 (MQ=255) ggAGCCATCCCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATg < 2:528277/151‑1 (MQ=255) ggAGCCATCCCCAGCAGGATCATGCCGCCCGGCATACGCTGGGCCGTCAAAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGAAAACCCTTC‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGata < 2:88543/144‑1 (MQ=255) aGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATg > 2:314336/1‑149 (MQ=255) ccttctcCAGCAGGACCCTGCCGCCCGGCATCCGCTGGGCCGTCACAGAAGGGATATGCGAGCGCCCGTGGATGACCAGCGTCCCTTCGTTATCGGTAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGaa < 2:599942/148‑1 (MQ=255) aTCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGt > 2:882286/1‑151 (MQ=255) cccgaagggaccttGCCTTCCGGCACACGCGGGGCCTTCACGAAAGGGATAGCCGAGCCCCCGCCGACTACCACCGTCTCTGCATTAGCGGAAAACCCTCG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGc < 1:389356/138‑1 (MQ=255) agcagAATCATGCCGCCGGGCATACGGTGGGCCGTCACAGAAAGGATATGCGACCGCCCGTCGATGACCAGCGTCACTTCGTTATGGTTAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTc < 2:676482/150‑1 (MQ=255) cagGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAAc > 1:187532/1‑151 (MQ=255) ggATCATGCCGTCCGGCATCCGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAg < 2:418674/151‑1 (MQ=255) ggATCATGCCGCCCGGCATACGCTGGGCCGTCACAGAAAGGATAGGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACa < 2:336731/150‑1 (MQ=255) ggATCATCCCGTCCGGCATCCGCGGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCTTTATCGGTAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACa < 2:523703/150‑1 (MQ=255) gATCTTGCCGCCGGGCTTACGCGGGCCCGTCACAAAAAGGATATGCGAGCCCCCTTCGATGCCCGGCGTCATTTCGTTAGCGGTAAACCCTGG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACa < 1:403136/149‑1 (MQ=255) aTGCCGTCCGGCATCCGCTGGGCCGTCACAGAAAGGTTATGCGGGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCg < 2:60026/151‑1 (MQ=255) aTGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCg > 1:426783/1‑151 (MQ=255) cgcccGGCATACGCTGGCCCGTAACAGAAAGGATATGCGAGCGCCCTTCGATGCCCAGCGTCCCTTCGTTATCGAAAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTaa < 1:475512/148‑1 (MQ=255) cATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGgtgt > 2:418692/1‑150 (MQ=255) cATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGgtgt > 1:331272/1‑150 (MQ=255) cGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCa > 1:311940/1‑150 (MQ=255) gCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCaa > 1:221146/1‑150 (MQ=255) tGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAAc > 1:766772/1‑150 (MQ=255) ccGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAAttt > 1:304750/1‑151 (MQ=255) caGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCggg > 2:157744/1‑150 (MQ=255) aGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGAAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCggg < 2:536278/149‑1 (MQ=255) atatGCGGGCGCCCGCCGATGCCCAGCGTCATTTCGTTATCGGAAAACCCTGG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTggg < 1:794587/151‑1 (MQ=255) atatGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTgg < 2:840649/150‑1 (MQ=255) gCGAGCGCCCGTCGATGACCAGCTTCATTTCGTTATCGGTAAACCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTggg < 1:318778/147‑1 (MQ=255) gCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCg > 1:118979/1‑149 (MQ=255) aGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGc > 1:145926/1‑149 (MQ=255) gcCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAgttg > 1:595735/1‑151 (MQ=255) ccGTCGATGCCCAGCGTCCCTTCGTTATCGGTAAACCCTGG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAgttgtt < 1:815132/151‑1 (MQ=255) cGATGACCAGCGTCCCTTCTTTATCGGTAAACCCTTC‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGttgttg < 1:8345/148‑1 (MQ=255) gACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGc > 2:312536/1‑150 (MQ=255) ccAGCGTCACTTCGTTATGGTTAAACCTTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCa < 2:131613/149‑1 (MQ=255) gTTCTCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATgg > 1:366941/1‑151 (MQ=255) gTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATgg > 1:381563/1‑151 (MQ=255) tATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGAt > 1:461811/1‑151 (MQ=255) ggTAAACCCTTC‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGc < 2:419726/150‑1 (MQ=255) tAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGcc > 2:868761/1‑149 (MQ=255) gaaaaCCCTGCCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCg < 1:314244/147‑1 (MQ=255) aaaaCCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAgg > 2:802101/1‑151 (MQ=255) | GTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTG‑CCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGG > NC_000913/4055968‑4056262 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |