Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	41.3%	80.5 / 13.9	47	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/25);  new base G (19/0);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 5.06e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354141‑354435                                                                                                                                                    |                                                                                                                                                      ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTg                                                                                                                                                      <  2:865029/149‑1 (MQ=255)   ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg                                                                                                                                                  <  1:518224/151‑1 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTg                                                                                                                                        <  2:623891/150‑1 (MQ=255)               gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAG‑AAg‑cggggg                                                                                                                                       >  1:533395/1‑144 (MQ=255)               ccTGACGGTTATCACTTTATTATCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGGAGTGGCGGGGGGTGGGGGGaaggg                                                                                                                                                 >  1:96029/2‑137 (MQ=255)                        tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAggtg                                                                                                                            <  2:863711/150‑1 (MQ=255)                         aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAggtgg                                                                                                                           <  2:140392/150‑1 (MQ=255)                               ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAG‑ATG‑CGCTGAAAGGGGAggggggggggg                                                                                                                     >  2:593268/1‑150 (MQ=255)                                 aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAG‑ATG‑CGCTGAAAAGGGAgggggcggggggg                                                                                                                   >  2:581391/1‑148 (MQ=255)                                   tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAG‑ATGCCGCT‑AAAGGgcggggggcggggggggtt                                                                                                                >  1:428512/1‑146 (MQ=255)                                    ctcGGGCGCACTGACGATGTAATTAACGTTCCCGGGCATCGGCTGGGGACGCGCGAGGTTGAAGAGAGCATCCTCAGCCTTCCGGGCGTTGCGGCGGTGGTGGTGGGATGCGCGAAAC‑ATC‑CTCTCGAAGGGGGggtggcggagggggt                                                                                                                 >  1:161085/1‑145 (MQ=255)                                          cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGGAAG‑ATG‑CGCTGAAAGGGGAGGGGGCGGGGGGGGTTGTCAtt                                                                                                          >  2:4082/1‑150 (MQ=255)                                           gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGGGGGGGGGAAGAAGG‑GGCGGAAAGGGCGGGGGGGGGGGGGGTTGGTctt                                                                                                           >  1:74202/1‑147 (MQ=255)                                              cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAG‑ATG‑GGCTGAAAGGGGAGGGGGGGGGGGCGGTTGTCATTcc                                                                                                        >  2:435379/1‑148 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                   <  1:575493/151‑1 (MQ=255)                                                 aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAaga                                                                                                  <  2:135797/151‑1 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                              <  2:96746/150‑1 (MQ=255)                                                           tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGCAGTGGCGGGGGGTGGGGGGGAAG‑ATG‑GGCGGGCAGGGCGGGGGGGGGGGGGGGTTGTCATTCCGAAaaagaggg                                                                                             >  1:61223/1‑144 (MQ=255)                                                            aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAG‑AAG‑CGCTGAAAGGGGAGGGGGCGGGGGGGTTTGTCATTTCGAAAGAGAGGGACAGt                                                                                        >  1:373489/1‑150 (MQ=255)                                                              cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTg                                                                                     <  1:426495/151‑1 (MQ=255)                                                               gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                    <  2:248825/151‑1 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                  <  1:660111/150‑1 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAG‑ATG‑CGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTTATTCCGAAAGAGAGCGACAGTCTgg                                                                                    >  2:637562/1‑148 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGa                                                                                <  2:515701/151‑1 (MQ=255)                                                                        cATCGGCTGGGGACGCGTGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGGGGGGGGAAAG‑ATG‑CGGGGAAGGGGCCGGGGGGGGGTGCGGGTGTTGTTACGTAAGCGAGGGACAGGGTGGGAGAGCGTg                                                                           >  1:524266/1‑151 (MQ=255)                                                                            ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt                                                                       <  1:588540/151‑1 (MQ=255)                                                                             gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGAAAG‑GTG‑GGCTGAAAGGGCCGGGGGGGGGGGCGGTTGTCCTTTCGGAAGAGAGGGACAGGCTGGGAGATc                                                                              >  2:556768/1‑143 (MQ=255)                                                                               tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAG‑ATG‑CGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTgg                                                                     >  2:742116/1‑150 (MQ=255)                                                                                     cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                              <  2:137760/151‑1 (MQ=255)                                                                                     cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAG‑ATG‑CGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                              >  2:430676/1‑151 (MQ=255)                                                                                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAGGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                             <  1:744256/151‑1 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                         <  1:212861/151‑1 (MQ=255)                                                                                           agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAaga                                                        <  2:832682/151‑1 (MQ=255)                                                                                           agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAG‑AAT‑CGCTGAAAGGGCAGGTGGGGGGGGGGTTTGTCATTTCGAAAGAGAGCGAAAGTCTGGAAGATCGTGATGTGGCGCACCCGcca                                                          >  1:387735/1‑147 (MQ=255)                                                                                                gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                    <  2:557413/150‑1 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                  <  2:862465/151‑1 (MQ=255)                                                                                                  agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGCAAG‑CAG‑CGCTGAAAGGGCGGGGGGCGGGGGCGTTTTTCATTTCGAAAGAGAGCGACAGACTGTGCGGACGGGATGTGGGGCACCCGCAAGaggagggg                                                 >  1:826847/1‑149 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAG‑ATG‑CGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCCAGAGAAGGCGATTATGgcg                                        >  2:481787/1‑149 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAA‑AAG‑CGCTGGAAGGGGAGGGGGCGGTGGGGTGTGGCTTTTCGGAAGAGAAGGGGAGGCTTGGAGATGGGGGTGGGGGGCCCTCGCGAGAGAAGGGGGTTTTGTCGGtgg                                    >  1:468315/1‑151 (MQ=255)                                                                                                                gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                   <  1:556620/151‑1 (MQ=255)                                                                                                                    tCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAG‑AAG‑CGCTGGAAGGGGCGGGGGCGGGGGGGGTTGGCATTCCGGAAGAGAGGGGCAGTCTGGAAGATCGCGATGTGGCGGACTCGCAAGAGAAGGCGGTTATGGCGCcggggg                                 >  2:445743/1‑149 (MQ=255)                                                                                                                     ccGGGCGTTGCCGAAGTGGCGGCGGGTTGGGGGAAAG‑ATA‑CGCCGCAAGGGCCGGGGGGGGGGGCGTTTGTCATTCCGGAAACGAGCGGCAGTCTGGGAAATCGTGGTATGGCGCACTCGCCCGAGAAGGGGGATATGgccgcggggg                                 >  1:754995/1‑140 (MQ=255)                                                                                                                          cGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAg                         <  2:95263/151‑1 (MQ=255)                                                                                                                              gCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                      <  1:893547/150‑1 (MQ=255)                                                                                                                                       gCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg            <  1:365319/151‑1 (MQ=255)                                                                                                                                         ggtggtTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc          <  1:370896/151‑1 (MQ=255)                                                                                                                                                 gggTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc  <  2:500167/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAG‑ATG‑CGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354141‑354435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑