Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,284	C→A	G79W (GGG→TGG)	sapC ←	antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,284	0	C	A	100.0%	199.4 / NA	51	G79W (GGG→TGG)	sapC	antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/21);  total (30/21)

AGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAC  >  NC_000913/1354142‑1354428                                                                                                                                               |                                                                                                                                                 aGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCCAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGga                                                                                                                                             >  1:502611/1‑148 (MQ=255)    ccGTGTGTCGCCCCGGCGAAGGTGCTCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGaagaaag                                                                                                                                       <  1:470187/151‑1 (MQ=255)      gtgtgtCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAagaaagaa                                                                                                                                     <  1:781122/151‑1 (MQ=255)           tCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTc                                                                                                                                >  1:547435/1‑151 (MQ=255)                ccGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCAt                                                                                                                           >  1:645811/1‑151 (MQ=255)                     gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAgcgc                                                                                                                      >  2:33101/1‑151 (MQ=255)                     gAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAgcgc                                                                                                                      <  2:570529/151‑1 (MQ=255)                       aGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGcgcg                                                                                                                     >  2:848641/1‑150 (MQ=255)                       aGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGa                                                                                                                    <  1:630845/151‑1 (MQ=255)                           gCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCAt                                                                                                                >  1:85080/1‑151 (MQ=255)                              cAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAc                                                                                                             <  2:390014/151‑1 (MQ=255)                              cAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAc                                                                                                             <  2:111814/151‑1 (MQ=255)                               aGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAc                                                                                                             >  1:137981/1‑150 (MQ=255)                                    cAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACggcggc                                                                                                       >  2:430469/1‑151 (MQ=255)                                         cacaGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaata                                                                                                   >  1:802864/1‑150 (MQ=255)                                                cgtcgCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATa                                                                                            >  1:718021/1‑150 (MQ=255)                                                cgtcgCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGAGCAGCTCCGCTCATTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATa                                                                                            <  1:867088/150‑1 (MQ=255)                                                     cgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCgag                                                                                      <  1:533226/151‑1 (MQ=255)                                                        cAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGaaa                                                                                   <  1:636663/151‑1 (MQ=255)                                                          aGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAAtt                                                                                 <  2:206801/151‑1 (MQ=255)                                                              tCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTg                                                                             >  1:231587/1‑151 (MQ=255)                                                               caccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGCAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTgg                                                                            >  2:266980/1‑151 (MQ=255)                                                               caccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTgg                                                                            >  1:743753/1‑151 (MQ=255)                                                               caccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTg                                                                             <  2:848372/150‑1 (MQ=255)                                                                  cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCg                                                                         <  2:881136/151‑1 (MQ=255)                                                                  cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCg                                                                         >  2:508686/1‑151 (MQ=255)                                                                  cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCg                                                                         <  2:181628/151‑1 (MQ=255)                                                                               cccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGgc                                                             <  2:239719/150‑1 (MQ=255)                                                                                 cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGgcgc                                                           >  1:533462/1‑150 (MQ=255)                                                                                    ggTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc                                                       >  2:210351/1‑151 (MQ=255)                                                                                    ggTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc                                                       >  2:822079/1‑151 (MQ=255)                                                                                    ggTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc                                                       >  2:415234/1‑151 (MQ=255)                                                                                     gTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAcc                                                      <  1:134796/151‑1 (MQ=255)                                                                                       tGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAcc                                                      >  2:40503/1‑149 (MQ=255)                                                                                                   gCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAAt                                         >  1:668820/1‑150 (MQ=255)                                                                                                            aCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAca                               <  1:524557/151‑1 (MQ=255)                                                                                                              ggCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAcagc                             <  2:160484/151‑1 (MQ=255)                                                                                                                 ttAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAcagcc                            >  2:411874/1‑149 (MQ=255)                                                                                                                  tAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAcagcca                           <  1:13001/149‑1 (MQ=255)                                                                                                                     aaCATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTAGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGcccc                      >  1:824365/1‑151 (MQ=255)                                                                                                                       cATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCcgc                    <  2:593073/151‑1 (MQ=255)                                                                                                                        aTCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCcgcg                   >  2:731086/1‑151 (MQ=255)                                                                                                                           gcgTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCgcgc                  >  2:406038/1‑149 (MQ=255)                                                                                                                           gcgTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCgcgc                  >  2:32972/1‑149 (MQ=255)                                                                                                                            cgTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGc               >  2:526147/1‑151 (MQ=255)                                                                                                                             gTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGcc              <  1:338848/151‑1 (MQ=255)                                                                                                                                 gAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTAc          <  2:547576/151‑1 (MQ=255)                                                                                                                                      cgtcAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGcc     >  2:507970/1‑151 (MQ=255)                                                                                                                                      cgtcAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGcc     >  1:249100/1‑151 (MQ=255)                                                                                                                                       gtcAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCg    >  1:448429/1‑151 (MQ=255)                                                                                                                                         cAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAc  >  1:76136/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                 AGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAC  >  NC_000913/1354142‑1354428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑