Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,375,123	0	T	G	44.0%	149.3 / 17.4	85	G85G (GGT→GGG)	dcuD	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (6/41);  new base G (37/0);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 6.66e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTACCAGCGCCTC  >  NC_000913/3374976‑3375272                                                                                                                                                    |                                                                                                                                                           gatgTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTa                                                                                                                                                          <  1:753599/150‑1 (MQ=255)    gtgCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTAt                                                                                                                                                         >  1:15837/1‑148 (MQ=255)       cGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTGTGGCCg                                                                                                                                                    >  2:320421/1‑150 (MQ=255)             cTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTATGCCCg                                                                                                                                                    >  2:766977/1‑144 (MQ=255)               ggTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACAt                                                                                                                                              <  2:272797/148‑1 (MQ=255)               ggTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGGGGGGGGGTTTGGCCggttcaaggg                                                                                                                                           >  2:97252/1‑142 (MQ=255)                      gTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGGGGGGGGGTATGCCCGCCACATTGGGCG‑C‑At                                                                                                                                   >  2:44315/1‑150 (MQ=255)                         cGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAggg                                                                                                                               <  1:536684/151‑1 (MQ=255)                         cGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAggg                                                                                                                               <  1:131516/151‑1 (MQ=255)                          ggtggtGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTATGCCCGCTACATGGAGCG‑C‑ATAgggg                                                                                                                              >  1:822193/1‑151 (MQ=255)                              gtgtACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAg                                                                                                                          <  2:831305/151‑1 (MQ=255)                                gtACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATTTCCCCCCCATGGAGGG‑G‑CTAGGGGGCAgg                                                                                                                         >  1:870931/1‑149 (MQ=255)                                   cTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATTCCCGCTACATGGGGCG‑C‑GAGGGGGGCAGgcgc                                                                                                                      >  2:517918/1‑150 (MQ=255)                                     cGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTTTGCCCCCCCCCTGGGGCG‑CGAAAGGGGccgggggg                                                                                                                      >  2:536358/1‑143 (MQ=255)                                       aTCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTTTTGCCGCCACATGGGGGGCC‑AGAGGGGccg                                                                                                                           >  2:537867/1‑141 (MQ=255)                                        tCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTTTGGCCCCCACATGGAGCG‑C‑CTAGGGGGCAggtccg                                                                                                                     >  2:542049/1‑141 (MQ=255)                                         cccACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑gg                                                                                                              <  1:176179/151‑1 (MQ=255)                                           caccaAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTATGCCCCCCCCCTGGAGCG‑C‑ATAGGGGGCCGGcgcgcggggg                                                                                                               >  1:906437/1‑145 (MQ=255)                                             ccaAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGGGGGGGGGTTTGCCCGCTACATGGAGCG‑G‑ATAGGGGGCAGGCGCGCGAT‑gggg                                                                                                            >  1:849006/1‑147 (MQ=255)                                              caAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGGGGGCGGGTATGTCCGCTACATGGAGAG‑C‑CTAGGGGCCAGACGCGCGGT‑GGCGAg                                                                                                          >  2:138613/1‑150 (MQ=255)                                                 aaGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTg                                                                                                      <  2:335454/151‑1 (MQ=255)                                                   gcagcGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTATTCCCCCCACATGGGGCG‑C‑CTAGGGGGGAGTCGCGCGGT‑GGGGGGgttg‑tt                                                                                                   >  1:63437/1‑151 (MQ=255)                                                      gcGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAg                                                                                                <  2:319924/151‑1 (MQ=255)                                                      gcGGCTACTTGAGCGTCTATATTTATAATGAAATCCTGCGGATGCTGTCCAACCGCATTGCCGGATTGGGGCTCTCGATTAGGGCGGTGGGCGGTTAGGCACGCTATATGGAGCG‑C‑ATAGGGGCTAGTCGCGCGAT‑TGTGAGCTTG‑TTAAg                                                                                                <  2:111704/151‑1 (MQ=255)                                                            aCTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAgccg‑c                                                                                           <  1:832355/149‑1 (MQ=255)                                                            aCTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑ccc                                                                                         <  1:175138/151‑1 (MQ=255)                                                            aCTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑ccc                                                                                         <  2:25667/151‑1 (MQ=255)                                                             cTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTATTTCCGCTACCTGGGGCG‑C‑ATAGGGGGCAGGCGCGCGGT‑GGGGGGGTTG‑TTAAAACG‑ccc                                                                                         >  1:558622/1‑150 (MQ=255)                                                               tGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGt                                                                                       <  1:615743/150‑1 (MQ=255)                                                                   cgtcgATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATGCCCGCCCCCTGGAGGG‑C‑ATAGGGGGCAGGCGCGCGAT‑GGGGAGGTTG‑TTTAACCG‑CCCGTTtaa                                                                                   >  1:503619/1‑150 (MQ=255)                                                                     tcgATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTTTTCCCGCCCCATGGAGGG‑C‑CTAGGGGGCCGGCGCGGGAT‑GGGGGGGGTG‑TTTAGCCG‑cccccttaaa                                                                                  >  1:448965/1‑142 (MQ=255)                                                                       gATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGCGGGCGGGTATTCGCGCCACAAGGGGAG‑C‑AAAGGGGGCAGGCGCGCGCTGGGGGAGGTGT‑TTAACCCC‑CCGGTTaaaaac                                                                                >  1:486120/1‑148 (MQ=255)                                                                         tatTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATGCCCCCCACATGGGGCG‑C‑CTAGGGGGCAGTCGCGCGGT‑GGGGGGGTTG‑TTTAGCCG‑CCCGGTtaaa                                                                                  >  1:33925/1‑145 (MQ=255)                                                                          atTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCTGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATGGCCGCTACATGGAGGG‑C‑ATAGGGGGCCGGCGCGCGAC‑GGGGAGCGTG‑TTTAAGCG‑CCCCCTAAAAATCAtt                                                                            >  1:497084/1‑150 (MQ=255)                                                                          atTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATGGCCGCTACAAGGAGGG‑C‑CAAGGGGGCAGGCGCGGGGT‑GGGGAGGGTGTTTAGGCCC‑CCCGTTAAAA‑TCATtc                                                                           >  2:201188/1‑151 (MQ=255)                                                                            ttATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATtcg                                                                          <  1:61460/150‑1 (MQ=255)                                                                              ataATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATtcgctc                                                                       <  1:163755/151‑1 (MQ=255)                                                                              ataATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATtcgctc                                                                       <  1:369982/151‑1 (MQ=255)                                                                              ataATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATtcgct                                                                        <  2:363209/150‑1 (MQ=255)                                                                                aaTGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATtcgctcgc                                                                     <  1:96592/151‑1 (MQ=255)                                                                                    aaaTCCTGCGCATGCTGTCCACCCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGta                                                                 <  1:579262/151‑1 (MQ=255)                                                                                     aaTCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGtat                                                                <  2:665274/151‑1 (MQ=255)                                                                                      aTCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGAGGGGGGGTATGTCCGCTCCCTGGGGCG‑C‑GTAGGGGGCCTTCGCGCGGT‑GGTGGGGGGG‑TTAGGACG‑CCCGTTCAAAAACATttgctc                                                                       >  2:224110/1‑143 (MQ=255)                                                                                       tCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGtata                                                               <  2:394065/150‑1 (MQ=255)                                                                                        ccTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTTTTCCCGCCCCATGGAGCG‑C‑ATAGGGGGCAGGCGCGCGAT‑GGGGGGCTTG‑TTTAGGCG‑CCCCCTAAAAAACATTTGCTCCCCGGAtatt                                                             >  1:451420/1‑151 (MQ=255)                                                                                          tGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATGTCCCCCACATGGAGCG‑C‑CTAGGGGGCAGTCGCGCGAT‑GGGGAGCTTG‑TTTAGACG‑CCCGTTAAAAATCATTCGCTCGCCGTAtattat                                                           >  1:234493/1‑151 (MQ=255)                                                                                           gcgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTAtattat                                                           <  2:259392/150‑1 (MQ=255)                                                                                           gcgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTAtatta                                                            <  1:744684/149‑1 (MQ=255)                                                                                           gcgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATattatt                                                          <  2:158325/151‑1 (MQ=255)                                                                                            cgcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGGGGGGGGGTATGCCCGCCACATTGGGGG‑C‑CTAGGGGGCAGGCGCGCGGT‑GGGGAGCTTG‑TTTAGCCGCCCCGTTAAAA‑TCATTTGCTCGCCGTATattatt                                                          >  1:122597/1‑150 (MQ=255)                                                                                             gcATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATGCCCGCTACATGGGGCG‑C‑CTAGGGGGCCGTCGCGCGGC‑GGGGGGGTTG‑TTAAAACG‑CCCCCTTAAAAACCTTCGCTCGCCCGATattatttt                                                        >  1:567654/1‑149 (MQ=255)                                                                                              cATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTATTCCCGCCACATGGAGGG‑C‑CTAGGGGGCCGGCGCGCGGT‑GGGGAGGTTG‑TTTAAGCG‑CCCGGTAAAAAACATTTGCTCGCCGGATattttttt                                                        >  1:74752/1‑148 (MQ=255)                                                                                              cATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTg                                                       <  2:552668/151‑1 (MQ=255)                                                                                               aTGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGt                                                      <  2:652120/151‑1 (MQ=255)                                                                                                   tGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGTTTTGCCCCCCACATGGAGCG‑C‑CTAGGGGGCAGTCGCGCGAT‑GGGGGGGTTT‑TTTAGCCG‑CCCCCTTAAACTCATTTGCTCGCCGTGTattttttt                                                        >  1:351660/1‑143 (MQ=255)                                                                                                    gTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCgg                                                   <  1:881343/149‑1 (MQ=255)                                                                                                       cAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACt                                              <  1:535679/151‑1 (MQ=255)                                                                                                        aaCCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGCCTGCCCCCCCCCCGGCGCC‑C‑CTAGGGGCCCGCCCCGCCCG‑GGGGGGCCTG‑TTTAGCCC‑CCCCCCAAAACCCATTCCCCCCCCCCCTATTATTCTTTccg                                                   >  2:360075/1‑143 (MQ=255)                                                                                                          cctcATTGCCGGATTGGGACTGTCGATTATGGCGGTAGGTGGATATGCCCGCTACATGGGGCG‑C‑ATAGGGGCCACTCGCGCGAT‑GGTGAGTTTG‑TTATGCCG‑CCAGTTAAAACTCATTCGATCGCCGTATATTATTCTGTCGGCAACTTAc                                           <  2:534360/148‑1 (MQ=255)                                                                                                          ccGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATGCCCCCTACATGGAGCG‑C‑ATAGGGGGCCGTCGCGCGGG‑GGGGAGCGTT‑TTTAGGCG‑CCCGTTAAAAATCATTCGCTCGCCGCATATTTTTTTTTCGGGAAcct                                             >  1:308267/1‑147 (MQ=255)                                                                                                            gCATTGCCGGATTGGGGCTGTCGATTATGGCGGTCGGCGGGTATGACCGCCAGATGGAGCG‑C‑GTAGGGGGGAGGCGCGCGGG‑GGTGGGGTTG‑TTATGACG‑CCCCGTAAAAATCCTTCGCTCGCCGTCTATTTTTCTGTCTGcgaaatccg                                          >  2:497838/1‑142 (MQ=255)                                                                                                                 gCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTTTGCCCGCCCCATGGGGGG‑C‑ATAGGGGGCCGGCCCGCGAT‑GGTGGGCTTT‑TTTAACCC‑CCCCGTAAAAATCATTTGCTCGCCGCATATTTTTTTTTCGGCCAACTACGTCa                                       >  1:536636/1‑148 (MQ=255)                                                                                                                 gCCGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTATGGCCCCCACCTGGGGGG‑C‑GCAGGGGGCCGGCGCGCGAG‑GGGGGGGTTG‑TTTAGTCG‑CCCCTTAAAAATCATTTGCTCGCCGGATATTTTTTTTTCTGCCCCTTACGGCAt                                      >  2:876373/1‑149 (MQ=255)                                                                                                                 gCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCg                                    <  2:586452/151‑1 (MQ=255)                                                                                                                 gCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTATGGCCGCTACATGGAGCG‑C‑ATAGGGGGCCGTCGCGCGGA‑GGGGAGATTT‑TTTAACCG‑CCCGCTAAAAATCAATTGCTCGCCGGATATTTTTTTTTTCGGGACTTACGTCATCg                                    >  1:45100/1‑151 (MQ=255)                                                                                                                  ccGGATTGGGGCTGTCGATTATGGCGGTGGGCGGGTTTTCCCCCCACATGGAGCG‑C‑ATAGGGGGCAGGGGCCCCGT‑GGGGGGGTTG‑TTAAGCCG‑CCCCTTAAAAAACCTTCGCTCGCCCGATTTTTTTTTGTCGGCAACCTACGTCATCgg                                   >  1:373859/1‑151 (MQ=255)                                                                                                                   cGGATTGGGGCTGTCGATTATGGCGGTGGGGGGGTATTCCCCCTACAAGGAGGG‑C‑CAAGGGGGCAGGCGCGCGAT‑GGGGGGGTTG‑TTTAGCCG‑CCCGGTAAAAATCATTCTCTCGCCGGATATTTTTTTTTCGGCCACCTATGTCATCg                                    >  1:287459/1‑149 (MQ=255)                                                                                                                        tGGGGTTGCCAATTATGGCGGTGGGCGGTTATGCCCGCTGCATGCAGCG‑C‑ATAGGGGCCGCTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAGCCCCATTCGCGCGCCGTATATTATTCTGTCGTCGACTTACGTCATCGGCCaa                               <  2:13215/149‑1 (MQ=255)                                                                                                                         ggggCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCGACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GTTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAAt                             <  2:625233/150‑1 (MQ=255)                                                                                                                         ggggCTGTCGATTATGGCGGGGGGGGGGGATGTCCGCCACATGGAGCG‑C‑GTAGGGGGCAGGCGCGCGAT‑GGGGGGGTTG‑TTTAGCCG‑CCCCGTAAAAAACATTTGCTTCCCCTGTATTATTCTTTGGGCCACTTActgcctctgcccaa                              >  2:82068/1‑136 (MQ=255)                                                                                                                           ggCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCAt                          <  1:11891/151‑1 (MQ=255)                                                                                                                           ggCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCAt                          <  1:692290/151‑1 (MQ=255)                                                                                                                           ggCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCAt                          <  1:792296/151‑1 (MQ=255)                                                                                                                            gCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATg                         <  1:58703/151‑1 (MQ=255)                                                                                                                                tCGATTAGGGCGGTGGGCGGTTATACCCGGTACATGGAGCC‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAGTCATGgcg                      <  2:740810/150‑1 (MQ=255)                                                                                                                                      aTGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGttt                <  2:889446/150‑1 (MQ=255)                                                                                                                                       tGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTttat              >  2:596109/1‑151 (MQ=255)                                                                                                                                       tGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTttat              >  1:108914/1‑151 (MQ=255)                                                                                                                                       tGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTttat              >  1:313178/1‑151 (MQ=255)                                                                                                                                        ggCGGTGGGCGGTTATGCCCTCTACATGGGGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTTGCCGTATATTATTCTGTCGGCATCTTACGTCATCGGCCAAATCATGGCGCAGTttat              <  2:778055/150‑1 (MQ=255)                                                                                                                                        ggCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTttatt             >  1:160571/1‑151 (MQ=255)                                                                                                                                             tGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTAcc          >  1:327542/1‑149 (MQ=255)                                                                                                                                               ggCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTACCAgcg      <  1:694464/151‑1 (MQ=255)                                                                                                                                               ggCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTACCAgcg      <  1:479199/151‑1 (MQ=255)                                                                                                                                                   gTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTACCAGCGCCTc  <  2:767111/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                           GATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTGGGCGGTTATGCCCGCTACATGGAGCG‑C‑ATAGGGGCCAGTCGCGCGAT‑GGTGAGCTTG‑TTAAGCCG‑CCCGTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTACCAGCGCCTC  >  NC_000913/3374976‑3375272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑