| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 44.4% | 20.6 / 13.3 | 27 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (11/4); new base A (0/12); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTC > NC_000913/357746‑358033 | aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAAcc < 1:396226/151‑1 (MQ=255) gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAAccg < 2:792969/151‑1 (MQ=255) cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAAccgc > 1:809644/1‑151 (MQ=255) cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTcc > 1:165323/1‑151 (MQ=255) ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTg > 2:342744/1‑151 (MQ=255) gCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑ggg > 2:695955/1‑151 (MQ=255) gtCAGGGGCTCCGGTGTTTCTTTCCCCCCAACACCCGCGCATTTACCCAACGCCATGTGAAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAag < 1:311373/143‑1 (MQ=255) aGCGGCTAACGTGATTCAAATCCGCCAACAATCGCGCATTGATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTC‑TGCCA‑GCTATTTTT‑CGGCCGCAACAAAAACGCCGTTCTGTCCAGTTGTGGC‑GGGGCAggacacaaagtg > 2:722223/1‑141 (MQ=18) ggCTCCCGTTATTCATTCCCCCCAACACCCGCGCATTTACCCACCCCCATGTGAAAAAATGCCTCCGCGGCGTCTGTC‑GCCCG‑GTTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATa < 2:775215/144‑1 (MQ=255) gTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAG‑aaaaaaaatttaacccc > 1:68215/1‑135 (MQ=25) tGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGGGG‑AAAAAACTTTatctc > 2:383828/1‑143 (MQ=25) ttCTTTCCCCCCAACACCCGCGCTTTTACCCACCCCCATGTGAAAAATTCCCTCGGGGGCGGCTGTC‑TCCCA‑GTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAt < 1:784696/149‑1 (MQ=255) cccccaaacaccCCCGCTTTTACCCACCCCAATGTCAAAAAAGCCCTCCGCGGCGTCTTTC‑TCCAA‑GTTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTtgtgtg < 2:217394/145‑1 (MQ=255) ccgcaacagCCGCCTATTTTACCAACGCCATGTTAAAAAATGCCTTCGGGGGGGGTGTCTTGCCG‑GCTTTTTTT‑CCGCCCAAAAAAACCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTtgtgtg < 2:876475/147‑1 (MQ=255) aacCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAG‑AAAAAACTTTTAACCCCCCCTGTTGTGTgggg > 1:796708/1‑143 (MQ=25) cacgcATTTACCCACCGCCGTGAACAAAATTCCTCCCGCGGCGCCTGTC‑TCCGA‑GCTGTTTCC‑CCCCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgct < 2:178621/149‑1 (MQ=255) ccAACGCCATTTCAAAAATTGCTTCCGCGGCGGGTGCC‑TCCCA‑GTTATTTTC‑CCGCCCCAAAAAATCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACa < 1:275398/147‑1 (MQ=255) ccccttgtgAAAAAAGCCCTCCGGGGGGGCTGTC‑TGCCA‑GCTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaa < 1:137734/146‑1 (MQ=255) tgtcaaaaaaTCCCTGCGCGGCGCCTTTC‑TCCCG‑GTTTTTTTC‑CCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAgg < 2:332029/144‑1 (MQ=255) gcaaaaaaaGGCCTTCGCGCCGGCTG‑C‑TGCCAGGCTTTTTTC‑CCGCGGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGa < 2:271228/140‑1 (MQ=255) aaaaaTGCTTGCGCGGCGGCTGTC‑GGCCA‑GTTATTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTg < 1:661949/150‑1 (MQ=255) aaaaaTGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGAGAAAAAAAATTTAAACCC‑CCACGTTTTGTGGGGATCGCTGCTGGGGAAAAAGTGGGAAGGGGAgggg > 1:895985/1‑148 (MQ=255) aaaaTGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGCAGAG‑AAAAAAAATTTAACCCCTCCTGTTGTGTGGGGAACGCCGCTGGGGAAAATATGGGAAGGGGAggggg > 1:832003/1‑147 (MQ=255) ggcggcTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGAG‑AAAAAAATTTAAACCCCCCATGTTGTGTGGGGAGAGCTGGTGGTGAAAATGTGGGAAGGGGAGAgcgggggaacaacc > 1:770323/1‑139 (MQ=255) gcgcTGTC‑CGCCC‑TCTTTTTTTCCCGCGGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa < 2:816091/143‑1 (MQ=255) ccA‑GTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCt < 1:294247/150‑1 (MQ=255) ccgcAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTc < 1:45382/150‑1 (MQ=255) | AGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTC‑TGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTC > NC_000913/357746‑358033 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |