| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,823,437 | 0 | C | T | 47.6% | 12.7 / 12.6 | 21 | intergenic (+152/‑78) | nadE/cho | NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair |
| Reads supporting (aligned to +/- strand): ref base C (10/1); new base T (0/10); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCGAACATTTAC > NC_000913/1823298‑1823587 | atTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCg > 1:620351/1‑150 (MQ=255) ggATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGAccc < 1:17451/151‑1 (MQ=255) gATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGAccc > 2:751387/1‑150 (MQ=255) tgtgCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGACCCGCct > 1:118764/1‑151 (MQ=255) gATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTaca > 2:95342/1‑149 (MQ=255) ccccTTATTCGCCCAAAAAAACTAGGAAAATTA‑AATACATTGCAGGAGTCAAATAGGCTTGACGGGCGTAGCCGGTC‑AGAGGGTGTACCTTTTTTCACCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGgataga < 1:82435/143‑1 (MQ=255) cctttATTCGACCAAAAAACCTAGGCAAATAA‑AATCAATTGCGGGATTGAAAAAGGCGTAAGGGGGATACCACGAA‑GGAGGGTATACCTTTTGTCTGCGCCCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAg < 1:796881/148‑1 (MQ=255) ttCGCCCTAAAAAACTATAAAAATTA‑AATACTTTGCGGGAGTAAAATAGGCTTGACGGGCGTAGCCCGTC‑GGAGGGTTTACCTTTTGTCATCGCCCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCAt < 1:8650/148‑1 (MQ=255) cttaaaaaCTATGAAAATTCAAAAATTTTGCGGGGGTGAAATGGGCTTGACGGGTGTA‑CACCTCAAGGCGTTGAAACTTTTTTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGt < 2:746228/146‑1 (MQ=255) cTATAAAACTATGCAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTc > 1:457762/1‑150 (MQ=255) aaCTATGAAATTTC‑AAGACATTGCAGGAGTCGATTGGGCGGGACGGGCGTAGCACGTC‑AGCGGGTTTACCTTTTGCAAGCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGt < 2:598055/150‑1 (MQ=255) attcAAATTC‑AATACAGTGCAGGAATCGAATAAGCCTTACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGCAGTCAACAGTGGTAc > 2:22915/4‑151 (MQ=255) gCAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACgg > 1:37939/1‑151 (MQ=255) cAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGc > 1:548089/1‑151 (MQ=255) tC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGttt > 1:462085/1‑150 (MQ=255) aaTAGGCTTGACGGGCGTAGCACTTA‑AGAGGGTGTACCTTTTGTCACCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTc < 1:602871/146‑1 (MQ=255) cccGTC‑AGAGGGTGTACCTTTTGTCACCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTAt < 1:773068/148‑1 (MQ=255) agtttgtACCTTTTTTCATCGCCCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAatat < 2:754977/144‑1 (MQ=255) aCGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATccc > 1:141843/1‑151 (MQ=255) ggTGTACCTTTTTTCATCGCCCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTAt < 1:551126/140‑1 (MQ=255) ggTGTACCTTTTGTCACCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCGa < 2:493728/151‑1 (MQ=255) cTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCGAACATTTAc > 2:511821/1‑151 (MQ=255) | ATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTC‑AATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTC‑AGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCGAACATTTAC > NC_000913/1823298‑1823587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |