Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,969,794	0	A	C	60.0%	0.8 / 18.5	20	G397G (GGT→GGG)	tap	methyl‑accepting protein IV
Reads supporting (aligned to +/- strand):  ref base A (7/1);  new base C (0/12);  total (7/13)
Fisher's exact test for biased strand distribution p-value = 1.03e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-02

GCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGC  >  NC_000913/1969644‑1969944                                                                                                                                                       |                                                                                                                                                       gcgGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCa                                                                                                                                                        <  2:686230/151‑1 (MQ=255)          cGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACt                                                                                                                                               >  1:877425/1‑151 (MQ=255)               tGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAAATTGCGCACTTCACCTGCCACTACCGc                                                                                                                                          >  2:751405/1‑151 (MQ=255)                 aCAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGACCCCGGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGTTGGCAAGATTGCGCCCTTCCCCTGCCACTACCg                                                                                                                                           <  1:789345/148‑1 (MQ=255)                           tCATGGTCGCGGCGGCGTTATTCCCCATTTTCGACCCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGcc                                                                                                                               <  1:519546/150‑1 (MQ=255)                             aTGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGcccc                                                                                                                             >  2:531985/1‑150 (MQ=255)                               ggccgcgGCGGCTTTTTTCCCCGTTTTCAAACCCGGCGGGACCCGATTGCCTGCCTCTTCGATCAGCCCTTTGACCTCTTTTGCTGCCTGCGCGCTCGGGCTGGCAAGATTGCCCACTTCACCTGCCACTACCGCAAAACCACGCCCCt                                                                                                                            <  2:905527/146‑1 (MQ=255)                                             tATTCACCAGTTTAGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGc                                                                                                            >  2:872679/1‑151 (MQ=255)                                                   ccAGTTCCGACCCCGGCTGGACCCGTTTATCTTCCTTTTCGTTCAGCCTTTTTACCTCTTTGGCCCCCCGCCCGCTCCGGCCGGAACGATTGCCCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGcc                                                                                                           <  2:392450/146‑1 (MQ=255)                                                       tttCGACCCCTGCTGGACACGTTTGACTGCCTCTCCTATCCCCCCTTTGATCTTTTTGGCTCCCGGCGCCCTCCGGCGGGCAAGTTTGCCCACTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTc                                                                                                      <  1:469088/147‑1 (MQ=255)                                                       ttccGACCCCTGCGGGCCACTATGGCCTGCCTCTCCCATCGCCCTTTTGTTCTTTTTTCCCCCCCGCGCGCTCGGCCTGCAAAGTTGGCCCCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCAc                                                                                                   <  2:82900/147‑1 (MQ=255)                                                          cGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGcc                                                                                               >  1:54382/1‑151 (MQ=255)                                                                                 ctgcctCTTCGATCAGCCTTTTTATCTTTTTTGCTGCCTGCGCGCTACGGCGGGAAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTgg                                                                         <  1:464129/150‑1 (MQ=37)                                                                                              cacccttttGTTCTCTTTTGCTGCCTGCGCGCTACGGCGGGAAAGATTGCCCTCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAAtt                                                           <  1:366180/145‑1 (MQ=25)                                                                                                    tttGCTCTTTTTTGCTGCCGCCGCGCCACGCCTGACAAGTGTCCCCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTc                                                          <  2:831766/146‑1 (MQ=21)                                                                                                    tttGATCTTTTTTGCTGCCGGCGCGCTCCGGCGGGAAAGTTTGCCCCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTc                                                      <  1:617949/150‑1 (MQ=25)                                                                                                     ttGATCTTTTTTGCGCCCCGCGCGCTACGGCGGGAAAGATGGCGCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTcc                                                         <  2:856556/146‑1 (MQ=25)                                                                                                         tcttTTTTGCTCCCTGCGCGCTCCGGCGGGAAAGTTTGCCCCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCATTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGAt                                                   <  1:872885/148‑1 (MQ=25)                                                                                                            ctTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCt                                             >  1:501136/1‑151 (MQ=255)                                                                                                                      ctgcGCCCTACGGCGGGCAAGATTGCGCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTc                                    <  2:307882/150‑1 (MQ=255)                                                                                                                                            ttGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCtggtgg             >  1:738768/1‑151 (MQ=255)                                                                                                                                                       aCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGc  >  1:837511/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                       GCGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGC  >  NC_000913/1969644‑1969944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑