Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	102,228	Δ1 bp	coding (1464/1476 nt)	murC →	UDP‑N‑acetylmuramate:L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	102,227	0	A	.	100.0%	352.8 / NA	72	coding (1463/1476 nt)	murC	UDP‑N‑acetylmuramate:L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (35/37);  total (35/37)

CTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTG  >  NC_000913/102079‑102373                                                                                                                                                     |                                                                                                                                                   cTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGgaagac                                                                                                                                                   >  1:224328/1‑149 (MQ=255) cTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGgaaga                                                                                                                                                    >  1:715266/1‑149 (MQ=255)       ccGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGa                                                                                                                                           <  1:279998/151‑1 (MQ=255)       ccGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGa                                                                                                                                           <  2:207805/151‑1 (MQ=255)         ggATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGAc                                                                                                                                          >  2:266614/1‑150 (MQ=255)         ggATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATAACt                                                                                                                                         >  2:95493/1‑151 (MQ=255)          gATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTg                                                                                                                                        >  2:2529/1‑151 (MQ=255)          gATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTg                                                                                                                                        >  2:715154/1‑151 (MQ=255)             ccGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGAt                                                                                                                                      <  1:374482/150‑1 (MQ=255)             ccGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATa                                                                                                                                     <  2:962731/151‑1 (MQ=255)              cGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATa                                                                                                                                     >  2:21992/1‑150 (MQ=255)              cGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATa                                                                                                                                     >  1:904826/1‑150 (MQ=255)              cGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATa                                                                                                                                     >  2:184230/1‑150 (MQ=255)               ggCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATaaa                                                                                                                                   <  1:222469/151‑1 (MQ=255)               ggCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATa                                                                                                                                     >  1:81586/1‑149 (MQ=255)                 cgcgGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAAt                                                                                                                                 <  2:818104/151‑1 (MQ=255)                  gcgGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATc                                                                                                                                <  2:902631/151‑1 (MQ=255)                   cgGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATcg                                                                                                                               >  1:104250/1‑151 (MQ=255)                          ccGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCt                                                                                                                        >  1:854082/1‑151 (MQ=255)                             agaTGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGtt                                                                                                                     >  2:535109/1‑151 (MQ=255)                               aTGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTtgg                                                                                                                   <  1:520931/151‑1 (MQ=255)                                 gCTGGCACCGGTATTAACCGGTAACGTCCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGTAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTtgggt                                                                                                                 <  2:602172/151‑1 (MQ=255)                                     gCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTtgggtggg                                                                                                              >  1:563073/1‑150 (MQ=255)                                      cACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGAc                                                                                                            >  2:306876/1‑151 (MQ=255)                                         cGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTc                                                                                                         <  1:873/151‑1 (MQ=255)                                                aaCCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGTCG‑GCAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACCTCAGc                                                                                                      >  1:651038/1‑147 (MQ=255)                                                aaCCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTg                                                                                                    >  2:602754/1‑149 (MQ=255)                                                 aCCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGc                                                                                                 <  2:41276/151‑1 (MQ=255)                                                 aCCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAAGTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGc                                                                                                 <  1:144756/151‑1 (MQ=255)                                                   cGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGGTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGATCgg                                                                                               <  1:991331/151‑1 (MQ=255)                                                      tAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGa                                                                                             >  2:458802/1‑150 (MQ=255)                                                       aaCGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGa                                                                                             >  1:76767/1‑149 (MQ=255)                                                           aCCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTtc                                                                                       >  1:408958/1‑151 (MQ=255)                                                                aTTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGa                                                                                  <  1:763398/151‑1 (MQ=255)                                                                     cGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAAtt                                                                               <  1:396133/149‑1 (MQ=255)                                                                     cGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAAtt                                                                               <  2:366404/149‑1 (MQ=255)                                                                     cGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTc                                                                              <  1:713479/150‑1 (MQ=255)                                                                        tCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTgg                                                                           <  1:905331/150‑1 (MQ=255)                                                                         cAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGgcg                                                                         <  1:729322/151‑1 (MQ=255)                                                                            ggggCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAg                                                                      <  2:66907/151‑1 (MQ=255)                                                                                 tGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGgtg                                                                 >  2:911572/1‑151 (MQ=255)                                                                                     aaTATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAg                                                             <  1:678943/151‑1 (MQ=255)                                                                                     aaTATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAg                                                             <  2:224354/151‑1 (MQ=255)                                                                                        atTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCg                                                          >  1:851278/1‑151 (MQ=255)                                                                                            gAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACt                                                      <  2:132257/151‑1 (MQ=255)                                                                                               aaaTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCg                                                   >  2:869840/1‑151 (MQ=255)                                                                                                aaTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGt                                                  <  2:563165/151‑1 (MQ=255)                                                                                                 aTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTg                                                 <  2:174342/151‑1 (MQ=255)                                                                                                  ttGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTg                                                 >  1:377426/1‑150 (MQ=255)                                                                                                   tGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGa                                                >  2:212519/1‑150 (MQ=255)                                                                                                      ccGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGc                                            <  1:732673/151‑1 (MQ=255)                                                                                                      ccGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGc                                            <  1:266584/151‑1 (MQ=255)                                                                                                            tttAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTAtt                                      >  2:180140/1‑151 (MQ=255)                                                                                                             ttAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTAtt                                      <  1:483849/150‑1 (MQ=255)                                                                                                              tAGCTGAAATCAAACTGAAGCCGCACACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTg                                     >  1:96622/1‑150 (MQ=255)                                                                                                               aGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGa                                    <  2:68325/150‑1 (MQ=255)                                                                                                                                   cGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGa                >  1:453956/1‑150 (MQ=255)                                                                                                                                    gCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGaaa              <  2:234300/151‑1 (MQ=255)                                                                                                                                    gCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGaa               >  2:747797/1‑150 (MQ=255)                                                                                                                                    gCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGaa               >  2:17583/1‑150 (MQ=255)                                                                                                                                      aaaCTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGaa               >  2:877032/1‑148 (MQ=255)                                                                                                                                      aaaCTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGaa               >  1:321287/1‑148 (MQ=255)                                                                                                                                      aaaCTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGaa               >  1:554886/1‑148 (MQ=255)                                                                                                                                      aaaCTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAaaga            <  2:21633/151‑1 (MQ=255)                                                                                                                                      aaaCTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAaaga            <  1:2529/151‑1 (MQ=255)                                                                                                                                        aCTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAaaga            >  1:535235/1‑149 (MQ=255)                                                                                                                                           ccGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGt         <  1:431227/149‑1 (MQ=255)                                                                                                                                           ccGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGt         <  2:910362/149‑1 (MQ=255)                                                                                                                                            cggcggAAG‑ACAACAGGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTACGCTGAGCGGGAAGTTTCTATGCATTCTGGCGCAGCGGTGTTAGCAGGACTGCGTGAAGGCGATATTGACGCGTATCCTGACGACCCGAAAGCAGTCg       >  1:768228/1‑150 (MQ=255)                                                                                                                                              gaggaAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACg    <  2:15213/151‑1 (MQ=255)                                                                                                                                               aggaAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGt   >  2:347917/1‑151 (MQ=255)                                                                                                                                                ggaAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTg  <  2:660251/151‑1 (MQ=255)                                                                                                                                                ggaAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTg  >  2:422319/1‑151 (MQ=255)                                                                                                                                                ggaAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTg  <  1:95627/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTG  >  NC_000913/102079‑102373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑