Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,984,441 | 0 | A | C | 43.1% | 106.7 / 14.4 | 58 | G384G (GGT→GGG) | yqeF | short chain acyltransferase |
Reads supporting (aligned to +/- strand): ref base A (31/2); new base C (0/25); total (31/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.01e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGC > NC_000913/2984295‑2984591 | tatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCt > 1:426682/1‑151 (MQ=255) atGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTg < 1:836500/151‑1 (MQ=255) tCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑cc > 2:588046/1‑151 (MQ=255) cATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑ccc > 2:132034/1‑151 (MQ=255) gaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGA‑CCCccgcc > 2:128085/1‑151 (MQ=255) aaTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCccgccg > 2:67631/1‑151 (MQ=255) aTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGa < 1:763910/151‑1 (MQ=255) ggAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGAt > 2:589261/1‑150 (MQ=255) gggAGCCAAAAAAATGTATGTCGACTTGCCACCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGAAAGGAGAAAATGAAGAGTTTTTCGTCCCTTTCAATGG‑TCAATGCCACCCCCTGG‑CCCCCGCCGATACAAAGCGtt < 2:443751/150‑1 (MQ=255) ggaacgcAGAAAATGTATGGCTAGTTGCCACCCATCATTATTCAACAAATTAA‑GGCGGGGGAAATTTTAGCAGTAAGGAGGAAATGCAGGGCTATTCGTCACTTGCAAGGG‑TAAGGCCCACCCCCCCG‑CCCCCGCCGATACAAAGCGtt < 1:849490/143‑1 (MQ=255) gAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCACGCCGATACAAAGCGTTGCCAGTCCtt > 1:138143/1‑151 (MQ=255) aaaaTGTATTTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATGGGTAAGGAGAAAATGCAGAGCTTTTCGTCACTTTAAATGG‑TAAATGCCCCCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTc < 2:81837/147‑1 (MQ=255) aaaaTGTATGTCGTTTTGCCAGCCATCATTATTAAACAGATAAATGAGGGCAGATA‑TTTATCGGTAGGGAGAAAGTGCAGAGCTTTTCGTCACGTTAAATGG‑TCAATGCCCCCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCtt < 2:281407/150‑1 (MQ=255) aaaaTGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCttt > 2:980991/1‑151 (MQ=255) tatgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGc > 2:258152/1‑151 (MQ=255) cGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAc > 1:726524/1‑151 (MQ=255) cGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAc > 2:810424/1‑151 (MQ=255) gACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg > 1:444760/1‑151 (MQ=255) gACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg > 1:754603/1‑151 (MQ=255) aCTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGt > 2:543782/1‑151 (MQ=255) ttccccaccaACATTATTCACCAGATAAAGGAGGGCAGATA‑TTTATCGGAAAGGAGAAAGTGCAGGGCTTTTCGTCACGTTCAATGG‑TAAACCCCCCCCCTTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg < 1:72036/141‑1 (MQ=255) ccagccaTCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGAAAATGCAGAGCTTTTCGTCCCGTTCAATGG‑TCAATCCCCCCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGtt < 2:17308/147‑1 (MQ=255) ccaaccaTCTTTATTCAACAGATAAAGGCCGGCAGATT‑TTTAGCGGAAAGGAGAAAATGGATTTTTTTTCGTC‑TGTTTAAAGG‑TTCATGCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc < 2:658685/149‑1 (MQ=255) ccaTTATTATCCAACAGATTAATGACGCCAGATA‑TTTATCGGTAGGGAGAAAATGCAGAGCTATTGGTCCTGTTCAAGGT‑TAAATCCCACCCCCGGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAtt < 1:371423/151‑1 (MQ=255) catcatTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACACTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAttt > 1:185110/1‑151 (MQ=255) attataCAACAGATAAAGGACGGAAGTTT‑TTTACCGGTAAGGAGAAAATGCAGAGCTTTTCGTCACTTTCAATGG‑TCAATGCCCCCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa < 1:886605/149‑1 (MQ=255) aaCAGATTAATGACGGCAGATA‑TTTGTGGTAAAGGAGGAAGGGCAGAGTTATTGGCCACGTTAAATGG‑TCAATGCCACCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCa < 2:83426/149‑1 (MQ=255) aCAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCag > 1:63212/1‑149 (MQ=255) aCAGATTAATGACGGCAGATA‑ATTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCag > 2:777038/1‑149 (MQ=255) ataaaggccGGAAGATT‑TTTATCGGTAAGGAGGAAATGCAGAGTTTTTCGTCACTTTCAATGG‑TAAATCCCACCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaaa < 2:259770/142‑1 (MQ=255) aTTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAc > 1:927909/1‑151 (MQ=255) aaTGACGGCAGATA‑TTTATGGGTAAGGAGAAAATGCAGAGCTTTTCGTCCCGTTCAATGG‑TCAATCCCCCCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAg < 1:701586/151‑1 (MQ=255) aCGGCAGATA‑TTTATCGGTAAGGAGAAAATGCAGAGCTTTTCGTCACGTTAAATGG‑TCAATGCCACCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAgg < 2:469681/148‑1 (MQ=255) cAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGc > 1:219013/1‑151 (MQ=255) aGATT‑TTTATCGGTAAGGAGAAAATGCAGACCTTTTCGTCACGTTCAATGG‑TCAATGCCCCCCCCGGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCa < 2:137182/151‑1 (MQ=255) ttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg > 1:955546/1‑150 (MQ=255) ggTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgcgc > 2:252824/1‑151 (MQ=255) aaTGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTg > 2:468723/1‑150 (MQ=255) tGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGAcc > 1:742442/1‑151 (MQ=255) agagCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCg > 2:221547/1‑149 (MQ=255) agagCTATTCCTCACGTTAAATGG‑ACATCCCCCCCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGa < 1:577560/150‑1 (MQ=255) gggCTTTTCGTCAGGTTAAATGG‑TAAGTCCCCCCCCCGGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAg < 2:307883/148‑1 (MQ=255) ctcTATTCGTCACGTACAATGG‑TCAATCCCATCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCg < 1:211952/145‑1 (MQ=255) cTATTCGTCACGTTCAATGG‑TCAATGCCATACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCg > 2:211976/1‑145 (MQ=255) cTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCg > 2:185311/1‑151 (MQ=255) ttctccACGTTAAATGG‑TCAATCCCCCCCCCGGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTg < 1:587303/141‑1 (MQ=255) ttCGTCACGTTCAATGG‑TCAATCCCACCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGAt < 1:898933/150‑1 (MQ=255) ttcttcactttaaTGGTTCAACGCCACCCCCGGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGa < 1:522781/138‑1 (MQ=255) cGTCACTTTAAAGGT‑CCAACGCCCCCCCCGGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcg < 1:928658/150‑1 (MQ=255) tCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCgcgc > 1:913344/1‑151 (MQ=255) ccctttaaaTGG‑TCAAGGCCACCCCCGGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcgcg < 2:162222/142‑1 (MQ=255) ccctttCAAGGG‑TAAATCCCCCCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCgcgc < 2:426081/146‑1 (MQ=255) gTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGccacc > 2:377383/1‑151 (MQ=255) aaTGT‑TAAATCCCCCCCCCGGC‑CCCCCGCCGATACAAAGCGTTGCCGGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattg < 2:992468/151‑1 (MQ=255) aaTGG‑TCACTCCCACCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcatt < 1:543691/150‑1 (MQ=255) aaTGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattg > 2:165123/1‑151 (MQ=255) aaTGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattg > 2:120346/1‑151 (MQ=255) cAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacattg > 2:10365/1‑151 (MQ=255) acacCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGAc > 1:301960/1‑151 (MQ=255) acCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGc > 1:567587/1‑151 (MQ=255) | TATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATA‑TTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGG‑TCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGC > NC_000913/2984295‑2984591 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |