Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,346,675 | 0 | T | G | 42.9% | 132.8 / 13.4 | 77 | T218P (ACC→CCC) | fumB | anaerobic class I fumarate hydratase (fumarase B) |
Reads supporting (aligned to +/- strand): ref base T (7/37); new base G (33/0); total (40/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.75e-15 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAT > NC_000913/4346527‑4346825 | aaCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTAc < 2:622632/151‑1 (MQ=255) cGCCTGACCCTGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGc < 1:182179/151‑1 (MQ=255) gCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGGTGCAGGACCGGGGGGAc > 2:188538/1‑148 (MQ=255) gCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTAACCAGGGGACg > 2:502188/1‑149 (MQ=255) gACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCAtt < 1:856862/149‑1 (MQ=255) cATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTtctc < 1:1564/151‑1 (MQ=255) cATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTtctc < 2:920309/151‑1 (MQ=255) aTGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTcg < 1:172646/151‑1 (MQ=255) tGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGGGGCCGCAACGGGGGGACGCATTTtct > 1:183689/1‑148 (MQ=255) cgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGGACGCATTTTTTcgacg > 2:411328/1‑149 (MQ=255) ttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGGTGCAGTACCGAGGGGACGCATTTTCTCGACGAg > 2:327197/1‑149 (MQ=255) ttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGGACGCCTTTTTTCGACGAgg > 1:372728/1‑150 (MQ=255) cccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTTGTTTCCGAAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGa < 2:4514/149‑1 (MQ=255) cTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCCGGACCGGGGGGACGCATTTTTTTGACGAGGAAGtt > 2:645110/1‑151 (MQ=255) cGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTc < 1:970902/151‑1 (MQ=255) ggCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGtt < 2:188296/151‑1 (MQ=255) gCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGttt < 1:815073/151‑1 (MQ=255) cATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCggg < 2:105444/151‑1 (MQ=255) aTCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGTCAGGC‑GCCATACCGGGGGGGCGCAATTTCTCTACGAGGGAGTTTTTTAGTTTgatgg > 2:920595/1‑145 (MQ=255) tAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑GAGGCGGCCGTACCGCGGGGACGCCTTTTTTTGACGAGGGAGGTTTTTCGGTTGTCggggggc > 1:222731/1‑146 (MQ=255) aaTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTca < 1:743978/150‑1 (MQ=255) aTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTcagc < 1:155495/151‑1 (MQ=255) aTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCCGCAGTACCGAGGGGACGCCTTTTTTCGGCGAGGAAGGTTTTCAGTTTGCCGGGGGGcagc > 2:918544/1‑151 (MQ=255) tAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGGGGGCGTACCGAGGGGAAGCCATTTCTCGCCGGGGGAGGTTTTTAGTTTGCCGGGAGTgag > 2:351889/1‑149 (MQ=255) aGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGGACGCCTTTTTTCGACGAGGGAGGTTTTTAGTTTGCCGGGAGGCAGCAggg > 2:651386/1‑149 (MQ=255) cgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCttt < 1:210094/151‑1 (MQ=255) gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTAACGAGGGGACGCCTTTTTTCGGCGAGGGAGGTTTTTAGTTTTCCGGGGGTCAGCAGGGCttt > 1:875908/1‑150 (MQ=255) gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGCAACGAGGGGACGCATTTTTTCGCCGAGGGAGGTTTTCCGTTTGGCGGGGGTCCACAggggctt > 1:161145/1‑146 (MQ=255) ttGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CCAGCTGCAGTCCCGACGGGACGCTATTTCTTCCCCCGGAAGGTTTGCAGGTTGTCGGGGGGCCGCAGGGGttttg > 1:6971/1‑148 (MQ=255) tGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGGACCGAGGGGACGCATTTTCTTGACGAGGGAGGGTTTTAGTTTGTCCGGAGGCAGCAGGGCttttggt > 1:293046/1‑147 (MQ=255) tGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGGACCCCGGGGACGCATTTTCTTTACGGGGAAGTTTTTTAATTTGCCGGGAGGCCGCCGGGGTTTGGtt > 2:765356/1‑151 (MQ=255) cTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCCGCAGTACCGCGGGGACGCATTTTCTCTACGAGGGAGGTTTTCAGTTTGCCGGGGGTCCGCAGGGCCTTgggt > 1:37225/1‑147 (MQ=255) cTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGGTGCCGGACCGGGGGGACGCCTTTTTTCGCCGAGGAAGGTTTTCCGTTTGCCGGGGGTCAGCAGGGGTTTGGgtt > 1:271100/1‑146 (MQ=255) tgccGGCTTCCAGGTTTGTTTCCGCAGACGTTCAACCAATCATAAACGCGATAATGTACGGCGTG‑CATTCTGCAGTACCGAGGGTACTCATTTTCTCGACGAGGAAGTTATACAGTTTGCCGGGAGTCTGCAGGGCTTTGGTTTCCTgg < 1:645913/146‑1 (MQ=255) ggTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑GAGGCTGCAGTACCGAGGGTAAGCATTTTCTCGACGGGGGAGGTTTTTAGGTTGTCGGGGGGCAGCAGGGCTTTGGTTTTCTggggg > 2:960679/1‑145 (MQ=255) gTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt < 2:68727/151‑1 (MQ=255) tCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACg < 1:503443/150‑1 (MQ=255) cAGGTTGGTTTCCGCAGACGTACCTCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGt < 1:80645/150‑1 (MQ=255) cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGt < 1:502106/150‑1 (MQ=255) cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGt < 2:899451/150‑1 (MQ=255) tggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTttgt < 2:531025/150‑1 (MQ=255) ggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑GAGGCTGCAGTCACGCGGGGACCCATTTTCTCGACGGGGAAGTTTTTTAAGTTTTCGGGGGGCAACAGGGGTTTGGTTTCCTTGGAGAGGTACGGTttggt > 2:302669/1‑148 (MQ=255) ggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGTTTTTTCGTTTGCCGGGGGTCAGCAGGGCTTTGGGTTCCTGGTAGAGATACGTTttgtt > 2:225988/1‑150 (MQ=255) gttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGGACGCATTTTTTCCACGAGGAAGGTTTTTCGTTTGCCGGGGGTCCGCCGGGCCTTTGTTTCCCGGGAGAGATAAGtttttt > 2:751683/1‑146 (MQ=255) ttCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGGTGCAGGAACGCGGGGACGCATTTTTTCGACGGGGGGGGTTTTCAGTTTTGCGGGGGTCAGCAGGGGGTTGGTTTCCTGGTAAAGGTACGGTttgtttgg > 1:714418/1‑147 (MQ=255) ccGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCag < 2:937008/150‑1 (MQ=255) ccGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCag < 2:204773/150‑1 (MQ=255) cAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGGACGCCTTTTTTCGACGAGGAAGTTTTTTAGTTTGCCGGGGGTCAGCAGGGCTTTGGGTTTCTGGTAGAGATGCGTTTTTTTGGCAGAgcc > 2:993957/1‑151 (MQ=255) cGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTAACCAGGGGACGCATTTTCTCGACGAGGGAGGTTTTCAGTTTGCCGGGAGTCAGCCGGGGTTTGGGTTTCTGGTAGAGATACGTTTTGTTGGCAGAgcc > 2:77079/1‑147 (MQ=255) tACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCCAGGGGACGCAATTTTTCGACGAGGGAGTTTTTTTGGTTTGCGGGGGGCAGCCGGGGTTTTGGTTTCTGGGAGAGATACGGTTTTTTGGGAGAgccgccgcc > 1:759458/1‑151 (MQ=255) aCCGCTAATCACAAACGAGATATGGTACGGGGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGGGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCGGGTAGAGATACGTTTTGTTGGCAGAgccgccgc < 1:405888/149‑1 (MQ=255) aCCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTAACCAGGGGACGCATTTTCTCGACGAGGGAGGTTTTTAGTTTTCCGGGGGTCAGCCGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGGAGAgccgccgcc > 2:365840/1‑150 (MQ=255) ccAATCACAAACGCGATATGGTACGGCGGG‑CAGGCCTTAGTACCGAGGGTACGCCTTTTTTCCACGCGGAAGTTTTTTTATTTTGCGGGAGACATTCGGGCGTTGGTTTCCTGGTAGAGGTATGTTTTTTTTGGAGAGCCGCCGCCttt > 2:922815/1‑149 (MQ=255) ccAATCACAAACGCGATATGGTACGGCGGG‑CAAGGTGGAGGAACGAGGGGACGCCATTTTTTGACGAGGGAGGTTTTTAGGTTGCCGGGGGGTAGCCGGGCTTTGGTTTTCTGGGAGAGATAAGGTTTGTTGGGGGAGCCGCCGCCttt > 2:459733/1‑149 (MQ=255) aTCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaa < 1:174791/151‑1 (MQ=255) cacaAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaacg < 2:463130/151‑1 (MQ=255) cacaAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaacg < 1:556634/151‑1 (MQ=255) aaaCGCGATATGGTACGGCGGG‑CAGGCCGCAGGACCCAGGGGACGCATTTTTTTGACGAGGAAGGTTTTTCGGTTGCCGGGAGGCAGACGGGCTTTGGTTTTCTGGTTGAGGTTCGGTTTTTTTGCAGAGCCGCCCCCTTTcgccacg > 1:371225/1‑148 (MQ=255) aaCGCGATATGGTACGGCGGG‑CCGGCTGCAGTACCCGGGGGACGCATTTTTTCGACGAGGGAGTTTTTTAGTTTGCCGGGGGGCAGCAGGGCTTTGGTTTTCTGGGAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTtgcaacgcaa > 2:264667/1‑150 (MQ=255) gcgATATGGTACGGCGGG‑CAGGCTGCCGTACCGCGGGGACGCCATTTTTTGCCGAGGGAGTTTTTTAGTTTGCCGGGGGGCAGCAGGGCCTTGGTTTCCTGGTAGAGATACGTTTTTTTGGCAGCGCCGCCCCCCTTcgccacgc > 1:310282/1‑145 (MQ=255) gATATGGTACGGCGGG‑CCGGGTGCAGGACCGAGGGGGAGCAATTTTTTGACGGGGAAGGTTTTTTGTTTGCCGGGGGTGAGCCGGGGTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCCTTCGCAACGCCAAgg > 1:358265/1‑148 (MQ=255) gATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAAt < 2:616963/151‑1 (MQ=255) atatGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGTCGCCGCCTTTCGCAACGCAAAGGAAtt < 1:637016/151‑1 (MQ=255) ggTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGt < 1:896341/150‑1 (MQ=255) aCGGCGGG‑GAGGCCGGAGTACCCGGGGGACCCATTTTTTCGCCGAGGAAGGTTTTTAGTTTTCCGGGAGTCAGCCGGGCTTTGGGTTCCTGGGAGAGGTACGTTTTGTTGGCAGAGCCGCCGCCCTTTGCAACGCAACGGAATTTGTACTc > 1:713242/1‑151 (MQ=255) aGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATc > 2:497166/1‑151 (MQ=255) gCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTa < 1:787027/151‑1 (MQ=255) gCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTAcct < 1:247717/151‑2 (MQ=255) cAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTa > 2:264405/1‑133 (MQ=255) cAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTa < 1:264375/133‑1 (MQ=255) aGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACcgcg > 2:67327/1‑151 (MQ=255) gTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACcgcg < 1:805481/150‑1 (MQ=255) aCCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTAc < 2:734550/151‑1 (MQ=255) aCCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTAc > 1:79840/1‑151 (MQ=255) ccGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACa < 1:574887/151‑1 (MQ=255) gggTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTc < 2:478995/151‑1 (MQ=255) ggTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCg > 1:242252/1‑151 (MQ=255) tACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAt > 1:354889/1‑151 (MQ=255) | AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGG‑CAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAT > NC_000913/4346527‑4346825 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |