Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,346,6750TG42.9% 132.8 / 13.4 77T218P (ACC→CCC) fumBanaerobic class I fumarate hydratase (fumarase B)
Reads supporting (aligned to +/- strand):  ref base T (7/37);  new base G (33/0);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 5.75e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAT  >  NC_000913/4346527‑4346825
                                                                                                                                                     |                                                                                                                                                      
aaCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTAc                                                                                                                                                      <  2:622632/151‑1 (MQ=255)
  cGCCTGACCCTGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGc                                                                                                                                                    <  1:182179/151‑1 (MQ=255)
   gCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGGACCGGGGGGAc                                                                                                                                                      >  2:188538/1‑148 (MQ=255)
   gCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACCAGGGGACg                                                                                                                                                     >  2:502188/1‑149 (MQ=255)
       gACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCAtt                                                                                                                                                 <  1:856862/149‑1 (MQ=255)
          cATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTtctc                                                                                                                                            <  1:1564/151‑1 (MQ=255)
          cATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTtctc                                                                                                                                            <  2:920309/151‑1 (MQ=255)
           aTGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTcg                                                                                                                                           <  1:172646/151‑1 (MQ=255)
            tGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGGGCCGCAACGGGGGGACGCATTTtct                                                                                                                                             >  1:183689/1‑148 (MQ=255)
                cgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTcgacg                                                                                                                                        >  2:411328/1‑149 (MQ=255)
                  ttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGAGGGGACGCATTTTCTCGACGAg                                                                                                                                      >  2:327197/1‑149 (MQ=255)
                  ttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCCTTTTTTCGACGAgg                                                                                                                                     >  1:372728/1‑150 (MQ=255)
                    cccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTTGTTTCCGAAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGa                                                                                                                                    <  2:4514/149‑1 (MQ=255)
                      cTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCCGGACCGGGGGGACGCATTTTTTTGACGAGGAAGtt                                                                                                                                >  2:645110/1‑151 (MQ=255)
                          cGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTc                                                                                                                            <  1:970902/151‑1 (MQ=255)
                              ggCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGtt                                                                                                                        <  2:188296/151‑1 (MQ=255)
                               gCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGttt                                                                                                                       <  1:815073/151‑1 (MQ=255)
                                     cATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCggg                                                                                                                 <  2:105444/151‑1 (MQ=255)
                                      aTCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGTCAGGCGCCATACCGGGGGGGCGCAATTTCTCTACGAGGGAGTTTTTTAGTTTgatgg                                                                                                                  >  2:920595/1‑145 (MQ=255)
                                          tAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCGGCCGTACCGCGGGGACGCCTTTTTTTGACGAGGGAGGTTTTTCGGTTGTCggggggc                                                                                                             >  1:222731/1‑146 (MQ=255)
                                           aaTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTca                                                                                                            <  1:743978/150‑1 (MQ=255)
                                            aTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTcagc                                                                                                          <  1:155495/151‑1 (MQ=255)
                                            aTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGACGCCTTTTTTCGGCGAGGAAGGTTTTCAGTTTGCCGGGGGGcagc                                                                                                          >  2:918544/1‑151 (MQ=255)
                                             tAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGGGGCGTACCGAGGGGAAGCCATTTCTCGCCGGGGGAGGTTTTTAGTTTGCCGGGAGTgag                                                                                                           >  2:351889/1‑149 (MQ=255)
                                                  aGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCCTTTTTTCGACGAGGGAGGTTTTTAGTTTGCCGGGAGGCAGCAggg                                                                                                      >  2:651386/1‑149 (MQ=255)
                                                    cgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCttt                                                                                                  <  1:210094/151‑1 (MQ=255)
                                                     gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACGAGGGGACGCCTTTTTTCGGCGAGGGAGGTTTTTAGTTTTCCGGGGGTCAGCAGGGCttt                                                                                                  >  1:875908/1‑150 (MQ=255)
                                                     gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGCAACGAGGGGACGCATTTTTTCGCCGAGGGAGGTTTTCCGTTTGGCGGGGGTCCACAggggctt                                                                                                  >  1:161145/1‑146 (MQ=255)
                                                       ttGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCAGCTGCAGTCCCGACGGGACGCTATTTCTTCCCCCGGAAGGTTTGCAGGTTGTCGGGGGGCCGCAGGGGttttg                                                                                                >  1:6971/1‑148 (MQ=255)
                                                        tGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGACCGAGGGGACGCATTTTCTTGACGAGGGAGGGTTTTAGTTTGTCCGGAGGCAGCAGGGCttttggt                                                                                              >  1:293046/1‑147 (MQ=255)
                                                        tGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGACCCCGGGGACGCATTTTCTTTACGGGGAAGTTTTTTAATTTGCCGGGAGGCCGCCGGGGTTTGGtt                                                                                              >  2:765356/1‑151 (MQ=255)
                                                          cTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGCGGGGACGCATTTTCTCTACGAGGGAGGTTTTCAGTTTGCCGGGGGTCCGCAGGGCCTTgggt                                                                                              >  1:37225/1‑147 (MQ=255)
                                                              cTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCCGGACCGGGGGGACGCCTTTTTTCGCCGAGGAAGGTTTTCCGTTTGCCGGGGGTCAGCAGGGGTTTGGgtt                                                                                             >  1:271100/1‑146 (MQ=255)
                                                                tgccGGCTTCCAGGTTTGTTTCCGCAGACGTTCAACCAATCATAAACGCGATAATGTACGGCGTGCATTCTGCAGTACCGAGGGTACTCATTTTCTCGACGAGGAAGTTATACAGTTTGCCGGGAGTCTGCAGGGCTTTGGTTTCCTgg                                                                                        <  1:645913/146‑1 (MQ=255)
                                                                    ggTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGTACCGAGGGTAAGCATTTTCTCGACGGGGGAGGTTTTTAGGTTGTCGGGGGGCAGCAGGGCTTTGGTTTTCTggggg                                                                                     >  2:960679/1‑145 (MQ=255)
                                                                     gTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt                                                                                 <  2:68727/151‑1 (MQ=255)
                                                                         tCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACg                                                                              <  1:503443/150‑1 (MQ=255)
                                                                          cAGGTTGGTTTCCGCAGACGTACCTCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGt                                                                             <  1:80645/150‑1 (MQ=255)
                                                                          cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGt                                                                             <  1:502106/150‑1 (MQ=255)
                                                                          cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGt                                                                             <  2:899451/150‑1 (MQ=255)
                                                                               tggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTttgt                                                                        <  2:531025/150‑1 (MQ=255)
                                                                                ggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGTCACGCGGGGACCCATTTTCTCGACGGGGAAGTTTTTTAAGTTTTCGGGGGGCAACAGGGGTTTGGTTTCCTTGGAGAGGTACGGTttggt                                                                       >  2:302669/1‑148 (MQ=255)
                                                                                ggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGTTTTTTCGTTTGCCGGGGGTCAGCAGGGCTTTGGGTTCCTGGTAGAGATACGTTttgtt                                                                       >  2:225988/1‑150 (MQ=255)
                                                                                 gttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCCACGAGGAAGGTTTTTCGTTTGCCGGGGGTCCGCCGGGCCTTTGTTTCCCGGGAGAGATAAGtttttt                                                                        >  2:751683/1‑146 (MQ=255)
                                                                                   ttCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGGAACGCGGGGACGCATTTTTTCGACGGGGGGGGTTTTCAGTTTTGCGGGGGTCAGCAGGGGGTTGGTTTCCTGGTAAAGGTACGGTttgtttgg                                                                    >  1:714418/1‑147 (MQ=255)
                                                                                     ccGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCag                                                                  <  2:937008/150‑1 (MQ=255)
                                                                                     ccGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCag                                                                  <  2:204773/150‑1 (MQ=255)
                                                                                        cAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCCTTTTTTCGACGAGGAAGTTTTTTAGTTTGCCGGGGGTCAGCAGGGCTTTGGGTTTCTGGTAGAGATGCGTTTTTTTGGCAGAgcc                                                              >  2:993957/1‑151 (MQ=255)
                                                                                            cGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACCAGGGGACGCATTTTCTCGACGAGGGAGGTTTTCAGTTTGCCGGGAGTCAGCCGGGGTTTGGGTTTCTGGTAGAGATACGTTTTGTTGGCAGAgcc                                                              >  2:77079/1‑147 (MQ=255)
                                                                                              tACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCCAGGGGACGCAATTTTTCGACGAGGGAGTTTTTTTGGTTTGCGGGGGGCAGCCGGGGTTTTGGTTTCTGGGAGAGATACGGTTTTTTGGGAGAgccgccgcc                                                        >  1:759458/1‑151 (MQ=255)
                                                                                               aCCGCTAATCACAAACGAGATATGGTACGGGGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGGGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCGGGTAGAGATACGTTTTGTTGGCAGAgccgccgc                                                         <  1:405888/149‑1 (MQ=255)
                                                                                               aCCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACCAGGGGACGCATTTTCTCGACGAGGGAGGTTTTTAGTTTTCCGGGGGTCAGCCGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGGAGAgccgccgcc                                                        >  2:365840/1‑150 (MQ=255)
                                                                                                   ccAATCACAAACGCGATATGGTACGGCGGGCAGGCCTTAGTACCGAGGGTACGCCTTTTTTCCACGCGGAAGTTTTTTTATTTTGCGGGAGACATTCGGGCGTTGGTTTCCTGGTAGAGGTATGTTTTTTTTGGAGAGCCGCCGCCttt                                                     >  2:922815/1‑149 (MQ=255)
                                                                                                   ccAATCACAAACGCGATATGGTACGGCGGGCAAGGTGGAGGAACGAGGGGACGCCATTTTTTGACGAGGGAGGTTTTTAGGTTGCCGGGGGGTAGCCGGGCTTTGGTTTTCTGGGAGAGATAAGGTTTGTTGGGGGAGCCGCCGCCttt                                                     >  2:459733/1‑149 (MQ=255)
                                                                                                      aTCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaa                                                <  1:174791/151‑1 (MQ=255)
                                                                                                        cacaAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaacg                                              <  2:463130/151‑1 (MQ=255)
                                                                                                        cacaAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaacg                                              <  1:556634/151‑1 (MQ=255)
                                                                                                           aaaCGCGATATGGTACGGCGGGCAGGCCGCAGGACCCAGGGGACGCATTTTTTTGACGAGGAAGGTTTTTCGGTTGCCGGGAGGCAGACGGGCTTTGGTTTTCTGGTTGAGGTTCGGTTTTTTTGCAGAGCCGCCCCCTTTcgccacg                                              >  1:371225/1‑148 (MQ=255)
                                                                                                            aaCGCGATATGGTACGGCGGGCCGGCTGCAGTACCCGGGGGACGCATTTTTTCGACGAGGGAGTTTTTTAGTTTGCCGGGGGGCAGCAGGGCTTTGGTTTTCTGGGAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTtgcaacgcaa                                           >  2:264667/1‑150 (MQ=255)
                                                                                                               gcgATATGGTACGGCGGGCAGGCTGCCGTACCGCGGGGACGCCATTTTTTGCCGAGGGAGTTTTTTAGTTTGCCGGGGGGCAGCAGGGCCTTGGTTTCCTGGTAGAGATACGTTTTTTTGGCAGCGCCGCCCCCCTTcgccacgc                                             >  1:310282/1‑145 (MQ=255)
                                                                                                                 gATATGGTACGGCGGGCCGGGTGCAGGACCGAGGGGGAGCAATTTTTTGACGGGGAAGGTTTTTTGTTTGCCGGGGGTGAGCCGGGGTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCCTTCGCAACGCCAAgg                                        >  1:358265/1‑148 (MQ=255)
                                                                                                                 gATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAAt                                     <  2:616963/151‑1 (MQ=255)
                                                                                                                  atatGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGTCGCCGCCTTTCGCAACGCAAAGGAAtt                                    <  1:637016/151‑1 (MQ=255)
                                                                                                                      ggTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGt                                 <  1:896341/150‑1 (MQ=255)
                                                                                                                         aCGGCGGGGAGGCCGGAGTACCCGGGGGACCCATTTTTTCGCCGAGGAAGGTTTTTAGTTTTCCGGGAGTCAGCCGGGCTTTGGGTTCCTGGGAGAGGTACGTTTTGTTGGCAGAGCCGCCGCCCTTTGCAACGCAACGGAATTTGTACTc                             >  1:713242/1‑151 (MQ=255)
                                                                                                                                   aGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATc                    >  2:497166/1‑151 (MQ=255)
                                                                                                                                     gCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTa                  <  1:787027/151‑1 (MQ=255)
                                                                                                                                        gCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTAcct               <  1:247717/151‑2 (MQ=255)
                                                                                                                                         cAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTa                                >  2:264405/1‑133 (MQ=255)
                                                                                                                                         cAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTa                                <  1:264375/133‑1 (MQ=255)
                                                                                                                                          aGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACcgcg             >  2:67327/1‑151 (MQ=255)
                                                                                                                                           gTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACcgcg             <  1:805481/150‑1 (MQ=255)
                                                                                                                                             aCCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTAc          <  2:734550/151‑1 (MQ=255)
                                                                                                                                             aCCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTAc          >  1:79840/1‑151 (MQ=255)
                                                                                                                                              ccGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACa         <  1:574887/151‑1 (MQ=255)
                                                                                                                                                  gggTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTc     <  2:478995/151‑1 (MQ=255)
                                                                                                                                                   ggTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCg    >  1:242252/1‑151 (MQ=255)
                                                                                                                                                     tACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAt  >  1:354889/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAT  >  NC_000913/4346527‑4346825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: