| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 102,228 | Δ1 bp | coding (1464/1476 nt) | murC → | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 102,227 | 0 | A | . | 98.1% | 240.8 / ‑3.3 | 52 | coding (1463/1476 nt) | murC | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (27/24); minor base C (1/0); total (28/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTC > NC_000913/102079‑102367 | cTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGgaagac > 2:128762/1‑149 (MQ=255)cTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGgaagac > 1:465216/1‑149 (MQ=255) ggATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACt < 1:625148/151‑1 (MQ=255) gATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTg < 2:713167/151‑1 (MQ=255) gcgGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATc > 2:544374/1‑151 (MQ=255) aGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTc > 1:849058/1‑151 (MQ=255) gCCGGGATGCTCTAACCGGGATTAACCGCTAACGACCCGATTCTCGTCCAGGCGGCTGGGAATATTGGAAAAACTGCCCGCTCTTTAGCTGCAATCCACCTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATACAATCGCGGTc > 2:21918/1‑150 (MQ=255) ccGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCt > 1:306157/1‑151 (MQ=255) aTGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTtgg > 2:854586/1‑151 (MQ=255) gCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTtgggt > 1:290265/1‑151 (MQ=255) catcGGTAATAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTGGCTGAATTGAACCTGAAGCCGCAAACTCCGGAGGAAGCA‑AACACGCCTGATAAAATCGCGGCCCTGTTGGGCGGGa > 2:210570/4‑150 (MQ=255) ggTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTTGGTGGGACCTcc > 1:208414/1‑151 (MQ=255) ttAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTg > 1:723383/1‑151 (MQ=255) aaCCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTg > 1:241856/1‑149 (MQ=255) ccGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCg < 1:610438/151‑1 (MQ=255) aaCGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAg < 1:160522/151‑1 (MQ=255) cGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGtt > 2:52487/1‑151 (MQ=255) gACCTGATTCTCGTTCAGGGGTCTGGTAATATTGGAAAAATTGCCCGTTCTTTCGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGttt > 2:687816/1‑151 (MQ=255) ccTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTtct > 2:793209/1‑151 (MQ=255) gATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTg < 2:860008/151‑1 (MQ=255) ctcGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAAtt > 1:515350/1‑151 (MQ=255) cGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAAtt < 2:638917/149‑1 (MQ=255) ttCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTgg > 1:813807/1‑151 (MQ=255) ttCAGGGGGCGGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACGCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCATGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCt > 2:433954/1‑149 (MQ=255) cAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGgc < 1:548767/150‑1 (MQ=255) aGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGgcgc < 1:794690/151‑1 (MQ=255) gggggCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCa < 1:685536/151‑1 (MQ=255) gggCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGc > 1:446273/1‑151 (MQ=255) gCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCgg > 1:38898/1‑151 (MQ=255) cTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGgt > 1:564534/1‑151 (MQ=255) atatTGGAAAAATTGCCCGTTCTTTAGCTGAAATCCAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGc < 1:270161/151‑1 (MQ=255) atatTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGc < 2:480309/151‑1 (MQ=255) accttGCAAACACTGCCGGTTCTTTAGCTGAAACCCAACTGAAGCCGCAAACTCCGGCGGTAG‑ACAACATGACCGATACAATCTCGGCCCTGTTGGGACGCACCGCCGCTGCGCCGGGAGTTTCTCTCAATTCTGCCGGAGAGGGGTTacc > 2:100496/4‑149 (MQ=255) aaaaaTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTg < 1:735537/151‑1 (MQ=255) aaaaTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGc < 2:234421/151‑1 (MQ=255) acgtcacGTTCTTTAGCTCAAATCAAACTGTAGCCGCAACCCCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCGGTTGGGTGGGACCGCCGCTGAGCGGGAAGTTTCTCTGAATCCTGGCGCAGCGGTGTTAGCCGGACTGCGTg < 2:744856/145‑1 (MQ=255) ctgTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATCACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGc > 2:180673/3‑151 (MQ=255) ccGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGCGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTTTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAgg > 2:707105/1‑150 (MQ=255) gTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCgg > 1:269100/1‑151 (MQ=255) tAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTg < 1:813894/150‑1 (MQ=255) tAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTg < 1:757756/150‑1 (MQ=255) aGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGC‑‑GAAGGCGGTATTGAcgc < 2:176999/151‑1 (MQ=255) gCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGAcg < 1:257215/151‑1 (MQ=255) gAAATCAAACTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGt < 1:790975/151‑1 (MQ=255) aCTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTc > 1:567464/1‑151 (MQ=255) aCTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTc > 2:176793/1‑151 (MQ=255) cTGAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCg > 1:256199/1‑151 (MQ=255) gAAGCCGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGAc > 1:513248/1‑151 (MQ=255) ccGCAAACTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGa < 1:489666/151‑1 (MQ=255) aaaCTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCAGCTGAGCGGGAAGTTTCTCTGCATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTGTCCTGTCGACCCg > 2:72837/1‑146 (MQ=255) aaCTCCGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAaagaa < 2:671183/151‑1 (MQ=255) ccGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGt < 2:813553/149‑1 (MQ=255) ccGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTc < 2:258894/150‑1 (MQ=255) ccGGAGGAAG‑ACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTc < 1:146473/150‑1 (MQ=255) | CTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTC > NC_000913/102079‑102367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |