Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091317,0410GC43.9% 48.1 / 20.9 41intergenic (‑81/‑448)mokC/nhaAregulatory protein for HokC, overlaps CDS of hokC/sodium‑proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (16/7);  new base C (0/18);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 2.56e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTAT  >  NC_000913/16896‑17186
                                                                                                                                                  |                                                                                                                                                  
tgctTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATAACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCt                                                                                                                                                 >  2:152787/1‑149 (MQ=255)
tgctTCATTGCTCTCTTCTCCTTGACCTTACGGCCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGCCAAACAGCCTc                                                                                                                                                >  2:641699/1‑150 (MQ=255)
         gCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACccgc                                                                                                                                     >  1:188075/1‑151 (MQ=255)
                  tcCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCtattat                                                                                                                            >  1:478646/1‑151 (MQ=255)
                    cTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTatat                                                                                                                          >  1:677641/1‑151 (MQ=255)
                         ccTTCCGGAAAGTAAGAGGCCCTCTACAGGTGTTCAGAAAATAGGAGGCCGGGGTTGGATAAAAAAAAACCCCTCGGGCTTTTTCTCTATCTGCCGTCCAGCAAATGCCTGAAACAGACAGCCAAAAGCACCCGCCGCTATTATATCGct                                                                                                                      <  2:797674/150‑1 (MQ=255)
                           ttACGGTCAGTAAGAGGCAATCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGctct                                                                                                                    >  2:250903/1‑150 (MQ=255)
                            tACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCActctct                                                                                                                  >  1:559921/1‑151 (MQ=255)
                               ggTCATAAAGAGCCACTCTACATTTGTTCAGCATAAGGGGGCCTTGGGGTTGATGAAAAAATTCCCCTCGGGTTTTTTCTCTACCTCCCGTTCATTTAATCCCGGAGCCAGACACCCTAAACCACCCGCCGCTATTATATCGctct                                                                                                                    <  2:365932/146‑1 (MQ=255)
                               ggTCAGTAAGAGGCACTCTACATGTTTTCAGCATATAGGGGCCCTGGGGTTGATGTTAAAATCTCACCCGGGGTTTTTCTCTTTCTGCCTTTCAGCAAATCCCGGAGACAGACACCCTCAAGCACCCGCCGCTATTATATCGctctct                                                                                                                  <  1:356089/148‑1 (MQ=255)
                                                ctACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACACGCCGCTATTATATCTCTCTCTTTAACCCATTTTGTTTTa                                                                                                <  1:609723/149‑1 (MQ=255)
                                                     tgttTTCAGCATATAGGAGGCTGGGGGTGGAGGAAAAAATCTCACTCGGGTTTTTTCTCTTCCCGCCTTTCAGCAAATGCGTGACAAAACCACCTTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATc                                                                                              <  2:699734/146‑1 (MQ=255)
                                                             gCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTg                                                                                 >  1:234749/1‑151 (MQ=255)
                                                                    ggaggCCTCGGGTTAATGAAAAAATATCCCTCGGGTTTTTTCTCTATCTGCCTTTCATCAAACGCCGAACACACACACCCTCAGGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACg                                                                           <  1:117098/150‑1 (MQ=255)
                                                                    ggagcCCTGGGTTTGATGGAAAAATACCCCGGGGGGTTTTCCTCTATCTCCCGTCCAGCAAAGGCCTGAGACAGACACCCTAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGaa                                                                               <  1:292165/146‑1 (MQ=255)
                                                                     gggcccTGGGTTTGATGATAAAATATCCCTCGGTTTTTTTCTTTCCCGCCCTTTCATTTAATCCCTGAAAAAAACACCCTAAACCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGa                                                                                <  2:1393/140‑1 (MQ=255)
                                                                      gaccccttgtgTTGATAGAAAAATCTCCCTCGGGCTTTTTCTCTCTCTGCCTTTCAGTTAATCCCGGAGACAACCACCCTAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTg                                                                                 <  2:804862/133‑1 (MQ=255)
                                                                       ggCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTc                                                                       >  1:211033/1‑151 (MQ=255)
                                                                          cTCGGTTTGATAGTAAAATATCACGCGGGGTTTTTCTCTATCGCCCGTTCAGCTAATGCCTGAGACACACAGCCTCAGGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGaa                                                                               <  2:587165/140‑1 (MQ=255)
                                                                          cTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGaa                                                                               >  1:587348/1‑140 (MQ=255)
                                                                             cGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAtt                                                                  >  1:276228/1‑151 (MQ=255)
                                                                               ggTTTATGAAAAATTCTCCCTCGGGTTTTTTCTTTATCTGCCTTCCATCTATTCCCTGAGACACACGCCTTAAGCCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAtttt                                                                <  2:427544/151‑1 (MQ=255)
                                                                                 ttGATGGTAAAATATCCCGCGGGCTTTTTCTCTATCGGCCGTTCAGCTAATGCCTGAGACAAACACCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAtttt                                                                <  1:243835/149‑1 (MQ=255)
                                                                                         aaaaTATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTa                                                       >  1:352270/1‑150 (MQ=255)
                                                                                         aaaaTACCCCTCGGGGTTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACACCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTa                                                       <  1:41099/150‑1 (MQ=255)
                                                                                         aaaaTACCACTCGGGTTTTTCCTCTGCTGGCCGTTCAACAACTCCCTAAGCAAGACAGCCTAAACCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTgg                                                           <  2:439194/146‑1 (MQ=255)
                                                                                               tCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAAtttttt                                                >  2:230822/1‑151 (MQ=255)
                                                                                                ccctggggttttttCTCTTTCTCCCGTTCAGTTAATCCCTGAGATAAACACCCTAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAAtttt                                                  <  2:432766/138‑1 (MQ=255)
                                                                                                    cGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATg                                            >  1:567099/1‑150 (MQ=255)
                                                                                                     gggttttttCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATg                                            <  1:569240/144‑1 (MQ=255)
                                                                                                     gggttttttCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACACCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGa                                           <  1:627052/145‑1 (MQ=255)
                                                                                                      ggttttttctttttctGCCGTTCAGCTAATGCCTGAGACAGACACCCTCAACCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAtt                                         <  1:860974/147‑1 (MQ=255)
                                                                                                      ggttttttCTCTCTCTGCCTTTCAGCTAATGCCTGAGACAAACACCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAt                                          <  2:138142/146‑1 (MQ=255)
                                                                                                       ggCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAttt                                        >  2:766419/1‑151 (MQ=255)
                                                                                                          ttttCTCTACCTGCCGTTCAGCTAATGCCGGAGACAGACACCCTAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAttt                                        <  1:401658/148‑1 (MQ=255)
                                                                                                                 tATCTGCCGTTAATCTAAGCCCTGAAACAGACCCCCTCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAattatt                                 <  1:19587/148‑1 (MQ=255)
                                                                                                                          ttCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAAt                       <  1:367280/149‑1 (MQ=255)
                                                                                                                            cAGCAAATGCCTAAGAAAGACACCCACAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTa                               <  2:817820/139‑1 (MQ=255)
                                                                                                                              gCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTATCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTAtct                 >  1:27733/1‑151 (MQ=255)
                                                                                                                                     ccTGAGACACACACCCTCACGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCAt           <  2:574519/150‑1 (MQ=255)
                                                                                                                                     ccTGAGAAAAACACCCTCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATc          <  1:498056/151‑1 (MQ=255)
                                                                                                                                             agacacCCTCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAattat  <  1:152828/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTAT  >  NC_000913/16896‑17186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: