Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,163	G→A	R158C (CGT→TGT)	sapF ←	antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,163	0	G	A	100.0%	186.4 / NA	51	R158C (CGT→TGT)	sapF	antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/23);  total (28/23)

CATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTT  >  NC_000913/1352016‑1352309                                                                                                                                                    |                                                                                                                                                   cATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTg                                                                                                                                                 >  1:561721/1‑151 (MQ=255)      atatACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGttttt                                                                                                                                            <  2:46910/151‑1 (MQ=255)        ataCGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGt                                                                                                                                          >  2:196106/1‑151 (MQ=255)         taCGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTc                                                                                                                                         >  2:204016/1‑151 (MQ=255)              aaTGCCCTTTTTTTCCTTTAATTCCTTCCTCAGCTTTCTCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGt                                                                                                                                    <  2:271412/151‑1 (MQ=255)              aaTGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGt                                                                                                                                    <  1:852061/151‑1 (MQ=255)                      gTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACa                                                                                                                             <  1:354412/150‑1 (MQ=255)                         tttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCCATAATGACTTTTGGGCGCAGTATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAAc                                                                                                                              <  2:332872/146‑1 (MQ=255)                          ttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATg                                                                                                                         <  1:345472/150‑1 (MQ=255)                              tGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGAt                                                                                                                    >  2:784243/1‑151 (MQ=255)                                  aTTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGt                                                                                                                 >  1:663154/1‑150 (MQ=255)                                      cAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTg                                                                                                            >  2:704393/1‑151 (MQ=255)                                           tCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTg                                                                                                       >  2:583250/1‑151 (MQ=255)                                                  aaTCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGt                                                                                                >  1:600448/1‑151 (MQ=255)                                                     cAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagc                                                                                             >  1:490713/1‑151 (MQ=255)                                                       aCTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagcag                                                                                           <  1:618978/151‑1 (MQ=255)                                                           cGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGccc                                                                                        >  2:135225/1‑150 (MQ=255)                                                               cGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCa                                                                                   <  1:230632/151‑1 (MQ=255)                                                                gCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCAt                                                                                  <  1:29567/151‑1 (MQ=255)                                                                     gACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGc                                                                              >  2:798450/1‑150 (MQ=255)                                                                            ccAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTc                                                                      >  1:849013/1‑151 (MQ=255)                                                                             cAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCa                                                                     <  1:593276/151‑1 (MQ=255)                                                                                  aaGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCaataat                                                                >  2:19187/1‑151 (MQ=255)                                                                                    gCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCt                                                              >  2:542982/1‑151 (MQ=255)                                                                                    gCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCt                                                              <  1:416208/151‑1 (MQ=255)                                                                                      gAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGt                                                            >  2:803866/1‑151 (MQ=255)                                                                                      gAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGt                                                            >  1:722527/1‑151 (MQ=255)                                                                                         ggCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTg                                                         <  1:674158/151‑1 (MQ=255)                                                                                            ttCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCg                                                       >  2:850769/1‑150 (MQ=255)                                                                                               aTCGGCAGTAGTGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTTCCAACATATGCGGATAGTAGCTGACGTGATCCGGTAGGAGCCCAACCATGCGCATCGTTTCAATAATCTGTTTGc                                                        <  2:46289/146‑1 (MQ=255)                                                                                                  ggCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGt                                                <  1:391084/151‑1 (MQ=255)                                                                                                   gCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGtt                                               <  2:440718/151‑1 (MQ=255)                                                                                                    cAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGTCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTc                                              <  1:204075/151‑1 (MQ=255)                                                                                                      ataatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCg                                            >  1:776296/1‑151 (MQ=255)                                                                                                       taatGACTTTTGGGAGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCgg                                           >  2:161225/1‑151 (MQ=255)                                                                                                          tGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTc                                        >  1:473182/1‑151 (MQ=255)                                                                                                               tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGt                                   >  1:534262/1‑151 (MQ=255)                                                                                                                        aaTATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCa                          <  1:568177/151‑1 (MQ=255)                                                                                                                         ctatCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTc                           >  1:458115/2‑149 (MQ=255)                                                                                                                         atatCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAg                         <  1:799741/151‑1 (MQ=255)                                                                                                                          tatCAGCGCGCGCGCCAGACCCAGATACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAgg                        >  1:656839/1‑151 (MQ=255)                                                                                                                          tatCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAgg                        >  1:741130/1‑151 (MQ=255)                                                                                                                               gcgcgcgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCa                    <  2:3713/150‑1 (MQ=255)                                                                                                                                  cgcgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTgg                >  1:570157/1‑151 (MQ=255)                                                                                                                                  cgcgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTgg                <  1:406159/151‑1 (MQ=255)                                                                                                                                    cgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGa               >  2:267486/1‑150 (MQ=255)                                                                                                                                    cgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGa               >  1:299619/1‑150 (MQ=255)                                                                                                                                      cgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGaaaa            <  1:36372/151‑1 (MQ=255)                                                                                                                                       gcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAAt           <  2:268833/151‑1 (MQ=255)                                                                                                                                        ccagacccagacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATc          <  1:59989/151‑1 (MQ=255)                                                                                                                                                agacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAAttt  >  2:587426/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTT  >  NC_000913/1352016‑1352309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑