| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,984,441 | 0 | A | C | 44.9% | 69.6 / 18.0 | 49 | G384G (GGT→GGG) | yqeF | short chain acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (23/4); new base C (0/22); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.34e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATT > NC_000913/2984294‑2984575 | atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACAccc > 2:827368/1‑151 (MQ=255) atGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCt < 2:512865/150‑1 (MQ=255) atGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCt < 1:692917/150‑1 (MQ=255) cATCAGATTGGAAAATTGAGGGAGCCCAGAAACTGTATGTCGAATCGACAGCAATCATTATTCAAC‑AGATTAATGACGGCAGATATTTAACGGTAAGGAGTAAACGCAGAGCAATTCGCCACGTTCAATTGTCAATCCCAAACCCTg > 2:735688/1‑147 (MQ=255) gagaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGccccc < 1:899468/150‑1 (MQ=255) aaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACaa > 2:795508/1‑150 (MQ=255) aGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGtt > 1:513530/1‑151 (MQ=255) aaaTGTATGTCGACTTGCCAGCCATCATTATTCAAA‑AAATAAATGACGGCAGATATTTATCGGTAAGGAGAAAATGAAGAGTTTTTCGTCACGTTCAATGGTCAATCCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTg < 1:877515/151‑1 (MQ=255) tatgtCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGc > 1:541595/1‑151 (MQ=255) tatgtCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGc > 1:696491/1‑151 (MQ=255) tgtCGCCTTGCCATCCTTAATTA‑TCAACAAGATAAAGGCGGGAAAATATTTATGGGTAAGGAGAAAATCCAGGACTATTTGTCCCGATCAATGGTCAGTCCCGCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGc < 2:771909/149‑1 (MQ=255) cGACTTCCCAGCCATCATTATTCAAC‑AGATAAATGACGGCAGATATTTATCGGTAAGGAGAAAATGCAGAGCTTTTCGTCACTTTCAATGGTCAATCCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAc < 1:530657/151‑1 (MQ=255) gACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATTTTTATCGGTAAGGAGGAAATGCAGAGCTTTTCGTCACGTTCAATGGTCAATCCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt < 2:41604/147‑1 (MQ=255) aCTGGCCAGCAATCATTATTAAAA‑AGATAAATGCCGGAAGATATTTATCGGAAAGGAGAAAATGCAAATCTTTTCATCACTTACAATGGCCAACCCCACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCgg < 2:818359/142‑1 (MQ=255) cagccaTAATTATACAAC‑ATATAAATGACGGAAGTTTTTTATGGGAAAGGAGAAAGGGCAGAGCTTTTCGTCACTTTAATTGGTCAATGCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCa < 2:154209/149‑1 (MQ=255) agccaTCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATCCAAAGCGTTGCCAGTCCTTTGGGGGCATTACGTTTCAcc > 2:210754/1‑150 (MQ=255) acccaTCTTTATACAAA‑AGATTAATGACGGCAATTTTTTAGGGGAAAGGAGAAAATGCAGATCTTTTCGTCACTTTCAAGGGTCAACCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGtt < 1:566895/143‑1 (MQ=255) cgatcatTATTCAAA‑AAAGAAATGGGGCCAGATTGTTTAGGGTAAAGTGGAAACGCATATCTAATCGCCACGTTCAGTGGTCAATCACTCCCCCTGCCACCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAt < 2:427084/148‑1 (MQ=255) ccaTCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAtt > 1:676320/1‑151 (MQ=255) tcatTTTTCAAC‑AAATTAATGACGCAAGATTTTTATCGGTAAGGAGAAAATGCAGAGTTTTTCGTCCCTTTAAATGGTCAATGCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc < 1:205904/150‑1 (MQ=255) ttattCAAC‑AGATTAATGCCGGCAGATATTTATCGGAAAGGAGAAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg < 2:578595/150‑1 (MQ=255) aGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCagag > 2:926727/1‑149 (MQ=255) gATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa > 1:294859/1‑150 (MQ=255) ttAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAcc > 2:705258/1‑151 (MQ=255) taaaggACGGCAGATATTTATCGGTAAGGAGAAAATGCAGAGCTTTTCGTCACTTTAAATGGTCAATGCCACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa < 1:827591/143‑1 (MQ=255) cgacatatatTTATCGGAAAGGAGAATATGAAGCGCTTTTCGTCACTTTCAATGTTAAATGCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGAt < 2:716217/143‑1 (MQ=255) ggaaGATATTTATGGGAAAGGAGGAAATGCAGACTTATTCTTCACTTTAAATGGTAAATCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGAtt < 1:492736/146‑1 (MQ=255) ggCAGATATTTATGGGAAAGGAGAAAATGCAGAGCTTTTCGTCACGTTAAATGGTCAATCCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTc < 1:624052/150‑1 (MQ=255) cAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGc > 2:255521/1‑151 (MQ=255) tatTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAAcc > 1:640737/1‑151 (MQ=255) ttttgcGGTAGGGAGGAAATGCAGGCTTTTTCGCCACGTTAAGTGTTCAATCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCg < 2:113731/145‑1 (MQ=255) ttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg > 1:802249/1‑150 (MQ=255) aaGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCt > 1:623034/1‑150 (MQ=255) aaGGAGAAAAGGCAGAGCTATTCGTCCCTTGAAATGGCAAATCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTa < 2:71547/151‑1 (MQ=255) gaggaAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATCACCCCCCACCATCTCATGACCCAGCGCCACCAGCACTCCCCAACCGCAACCCCCTATc > 2:348692/1‑150 (MQ=255) gaAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCggg > 2:21942/1‑150 (MQ=255) aaaTGCAGAGCTTTTCGTCACTTTAAAGGGTAAATCCCACACCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTg < 1:962347/151‑1 (MQ=255) cAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGAcc > 2:540882/1‑149 (MQ=255) gagCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGt > 2:269226/1‑151 (MQ=255) aTTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGAt > 1:187800/1‑151 (MQ=255) aTTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGAAACAAACCGTTGCCAGCCCTTTCGGGGCATTACTTTTCACCATTGCAGGAACCAGAAAAACCAGGATTCTGCAACCGAAAGCTCCTATCGGTTGACCGGGttc > 1:84238/1‑146 (MQ=255) ttCGTCACGTTAAATGGTAAATCCCACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGAt < 1:869984/150‑1 (MQ=255) tCGTCACTTTCAATGGTAAATCCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATc < 1:733769/150‑1 (MQ=255) gTCACGTTCAATGGTCAATGCCACAACCTGGAAACAGAAGATATAAAGAGTTGAAAGTAGTTTGAGGGAATTAAGTTTAAATATTTAATGAAGAAGAGAAAATAGGATTAGGTAATAGGAAGAGAATATAGGGTGATAGAGTGAGAtag > 2:304078/1‑147 (MQ=255) gTCACGTTAAATGGTCAATGCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcgc < 1:40754/150‑1 (MQ=255) cGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGccac > 2:184522/1‑151 (MQ=255) gtaaaaTGGCAAACGCCACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGccacc < 2:229163/147‑1 (MQ=255) aaTGGTCAACCCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGccacca < 2:867754/148‑1 (MQ=255) aaTGGTAAATCCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcatt < 1:715021/150‑1 (MQ=255) | ATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAAC‑AGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATT > NC_000913/2984294‑2984575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |