Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913300,7210GT41.0% 58.7 / 16.2 39Q72K (CAA→AAA) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base G (5/18);  new base T (16/0);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 9.98e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTCGATG  >  NC_000913/300584‑300863
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ggCCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAAAGAAAGCCCGGACGCCCCGCGCTTTTTTCgcgggggcc                                                                                                                                       >  1:260521/1‑143 (MQ=255)
 gCCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATGAAAACGAGTACGCCCCGCGCCCTTTTCGCGGGGGCCGGAt                                                                                                                                   >  2:352240/1‑151 (MQ=255)
    aCTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGTATTACAACCCGGACGCCCCGCGCCTTTTTCgcgggggcc                                                                                                                                       >  1:680372/1‑139 (MQ=255)
     cTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑aa                                                                                                                               <  1:904393/150‑1 (MQ=255)
         cAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCCGGAAGCCCCGCGGCTTTTTTGCGGGGGCCGTATC‑AAagggg                                                                                                                          >  1:6446/1‑149 (MQ=255)
         cAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCg                                                                                                                          <  1:647686/151‑1 (MQ=255)
                     cTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCCGGACGCCCCGCGCTTTTTTTGCGGCGTCCGTATC‑AAGGGCGGGGGGGCGGcc                                                                                                               >  1:123883/1‑150 (MQ=255)
                      tgatgaTAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGAAAACCAGTACGCCCCGCGCTTTTTTTGCGGGGGCCCGATC‑AAGGGGGGGGGGGGGTcc                                                                                                               >  1:515919/1‑149 (MQ=255)
                       gatgatAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAAATAAAAACAATACGCCCCGGGCCTTTTTGGCGGGGGCCCGATCAAAGGGGGGGGGGGGGTCCtt                                                                                                             >  1:535235/1‑151 (MQ=255)
                        atgatAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTg                                                                                                           <  2:748592/151‑1 (MQ=255)
                         tgatAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATTACAGCCCGTACGCCCCGCGCCTTTTTTGCGGCGCGCGGATC‑AAAGGCGGGGGGGCGGcc                                                                                                               >  2:105424/1‑146 (MQ=255)
                         tgatAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAAAGACAACCACAACGCCCCGCGCTTTTTTCGCGGCGGCCCTATC‑CAGGGCGGGGAGGGGGCCttt                                                                                                            >  1:618570/1‑149 (MQ=255)
                           atAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGAAAGCCAGTACGCCCCGCGCCTTTTTCGCGGCGGCCCGATC‑AAGGGGGGGGGGGGGGCCttttg                                                                                                          >  1:501377/1‑147 (MQ=255)
                                 cTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAAATACAGCCCGTACCCCCCGCGCGTTTTTTGCGGCGGCCGGATT‑CAGGGGGGGGGGGCGGCCTTTTGTAATGGCg                                                                                                  >  2:998686/1‑151 (MQ=255)
                                       aGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACcg                                                                                            <  1:155497/151‑1 (MQ=255)
                                           gggCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACcgacga                                                                                        <  1:365500/151‑1 (MQ=255)
                                                 ccTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATTACAGCCAGTTCGCCCCGCGCCTTTTTCGCGGGGGCCGGATT‑AAAGGCGGGGGGGCGGCCCTTTGCGAAGGGGGGACcggcggaaaa                                                                                    >  2:98920/1‑144 (MQ=255)
                                                            ggCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAggcggc                                                                        <  1:265844/150‑1 (MQ=255)
                                                                 tgtTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGAAAGCCAGTACGCCCGGCGCTTTTTTCGCGGCGTCCGTATC‑AAGGGGGGGGGGGCGTCCTTTGGCAATGGCGGGACCGACGGTATAACCATAGGCGGCGGTggg                                                                  >  1:829094/1‑151 (MQ=255)
                                                                   tttttGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATGACACCCAGTACCCCCCGCGCTTTTTTCGCGGCGTCCGTATC‑AAGGGGGGGGGGGGGTCCTTTTGCAATGGCGGGAACGACGATATAGCCCTAGGGGGGGTTggggg                                                                >  1:937345/1‑151 (MQ=255)
                                                                        gTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGctg                                                             <  2:811314/149‑2 (MQ=255)
                                                                          cGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGcttctt                                                          <  1:190948/150‑1 (MQ=255)
                                                                          cGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGcttctt                                                          <  1:666317/150‑1 (MQ=255)
                                                                                 gCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCa                                                   <  2:359427/150‑1 (MQ=255)
                                                                                    gAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACCCCCCGCGCTTTTTTCGCGGCGTCCGTATC‑AAGGGCGGGGAGGCGTCCCTTGGCAATGGCGGGACCGACGATATAGCCCTAGGCGGCGGTGGGGGGTTCTTCATGCCCtt                                                 >  1:53233/1‑149 (MQ=255)
                                                                                      gTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGt                                              <  2:472252/150‑1 (MQ=255)
                                                                                      gTGCCCCGGCGTTACTGGCGGTAAAGACAGCCAACAAGCCCCGCGCTTTTTTCGCGGCGGCCGGATC‑AAGGGGGGGGGGGGGGCCTTTTGTAAAAGCGGAAACCGCCAAATAGCACTAAGCGGCGGTGGGGGGGTCTTCATGcccattgt                                              >  2:832033/1‑144 (MQ=255)
                                                                                              gCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGc                                     <  1:491176/151‑1 (MQ=255)
                                                                                                            aaTGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTgccgggccgg                         >  2:57419/1‑149 (MQ=255)
                                                                                                            aaTGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCgg                         >  1:966780/1‑149 (MQ=255)
                                                                                                              tGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAg                      >  2:516035/1‑150 (MQ=255)
                                                                                                                 cAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGtttt                  <  2:260481/151‑1 (MQ=255)
                                                                                                                 cAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGttt                   <  2:256662/150‑1 (MQ=255)
                                                                                                                   gCCAGTACGCCGGCCGCCTTTTGCGCGGCGTCCGTCTC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGCCGATATAGCCACAGCCCGCGGTCGGGGCTGCTTCATTCCATTCGTACGCGTAGCGTGCCGTGCCGGTCGGTTTc                 >  2:425516/1‑150 (MQ=255)
                                                                                                                   gCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCa                <  1:588488/151‑1 (MQ=255)
                                                                                                                     cAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGAGGATATAGCCATAGGCTGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGc              <  2:824687/151‑1 (MQ=255)
                                                                                                                            cccGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATGGGCGGCGTTGGGTGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCTGt       <  2:176986/151‑1 (MQ=255)
                                                                                                                             ccGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTc      <  2:515789/151‑1 (MQ=255)
                                                                                                                                 cgcTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTCGATg  >  2:787947/1‑151 (MQ=255)
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GGCCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGTCGATG  >  NC_000913/300584‑300863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑