Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	941,713	0	T	G	47.8%	60.8 / 20.4	46	V252G (GTG→GGG)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (3/21);  new base G (22/0);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 2.95e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941569‑941862                                                                                                                                                 |                                                                                                                                                      actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTa                                                                                                                                                 <  1:585429/151‑1 (MQ=255)  ctcctcCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCtt                                                                                                                                                  >  1:836644/1‑149 (MQ=255)     ctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTa                                                                                                                                              >  2:146169/1‑150 (MQ=255)        cgcgCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCtt                                                                                                                                          <  1:471477/151‑1 (MQ=255)         gcgcAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAACACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGaagtccttttt                                                                                                                                           >  2:622102/1‑139 (MQ=255)           gcAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGGAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGa                                                                                                                                        <  1:233147/150‑1 (MQ=255)           gcAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGaa                                                                                                                                       <  1:307357/151‑1 (MQ=255)             aaaTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTATTATCtt                                                                                                                                          >  2:478079/1‑146 (MQ=255)              aaTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTTCTATCTTGAACAg                                                                                                                                    <  1:925187/151‑1 (MQ=255)              aaTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATTTTTAACa                                                                                                                                     >  1:216625/1‑150 (MQ=255)               aTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCTTTaa                                                                                                                                       >  1:12661/1‑147 (MQ=255)                 tGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGc                                                                                                                                  <  2:320614/150‑1 (MQ=255)                 tGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGc                                                                                                                                  <  1:310232/150‑1 (MQ=255)                   cGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGGTATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACg                                                                                                                               <  1:65651/151‑1 (MQ=255)                     gAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGcc                                                                                                                             <  1:509563/151‑1 (MQ=255)                     gAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTGAACAAGCACGcc                                                                                                                             >  1:768540/1‑151 (MQ=255)                      aaGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCa                                                                                                                            <  1:718461/151‑1 (MQ=255)                           ttGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAATTACTATCTTTAACAAGCCCGCCAGaaa                                                                                                                        >  1:277115/1‑150 (MQ=255)                             gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCt                                                                                                                     <  2:321525/151‑1 (MQ=255)                                    aCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGGTTACTATCTTTAACAGGCACGCCCGAAAACTAATGccc                                                                                                              >  2:335703/1‑151 (MQ=255)                                    aCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGCGGGGGGACTTACTTTCTTGAAAAAGCACGCCACAAATCTaaagtcc                                                                                                              >  1:291413/1‑146 (MQ=255)                                             ggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTGAACAAGGACGCCAGAAAACTAATGCCCGc                                                                                                            >  2:563178/1‑144 (MQ=255)                                                tGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCATGatcat                                                                                                    >  1:270260/1‑149 (MQ=255)                                                 gggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcat                                                                                                 <  1:586838/151‑1 (MQ=255)                                                   gCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCg                                                                                               <  2:870755/151‑1 (MQ=255)                                                          gCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCcgcg                                                                                        <  2:563951/151‑1 (MQ=255)                                                          gCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCcgcg                                                                                        <  1:351447/151‑1 (MQ=255)                                                          gCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATATTTTGTACAGGCACGCCAGAAACCTAAAGCCCGCCTGATCATAATCGATcccc                                                                                         >  2:535365/1‑148 (MQ=255)                                                             aCAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTAATATTTTTAAAAGGCCCCCCAGCAATCTAATGACCGCATGAACATCATCGATCCgcgc                                                                                       >  2:288572/1‑149 (MQ=255)                                                               aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTATATCTTTGACAGGGACGCCAGAAATCTAATGGCCGCATGATCATCATCGATCCGCGCtct                                                                                    >  2:236347/1‑148 (MQ=255)                                                                    gTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGac                                                                              <  1:436386/151‑1 (MQ=255)                                                                         atatCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGCTTACCCTTTTTGAAAGGCCCGCCCGAAAACTTATGCCCACATGATCACCATCGATCCCCCCCATACCGACAcc                                                                           >  1:631300/1‑149 (MQ=255)                                                                                   cAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGATTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTCTACCGACACCGGTGCCggg                                                                  >  2:834091/1‑148 (MQ=255)                                                                                      tAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCTTGAAAAAGCCCGCCAGAAAACTAATGCCCGCATGATTATCATCGATCCGCGCCATACCGCCACCGGTGGCGGGcgcg                                                              >  2:651432/1‑149 (MQ=255)                                                                                            ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGa                                                       <  1:335796/150‑1 (MQ=255)                                                                                            ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGTGACCTACCATCTTTTAAAGCGACGCCAGAAAACTTAATCCCCCATGGACCTTAACGCTTCGCGCCCTACCGAAACCGGAGCCTGgcgctgaga                                                          >  1:208613/1‑142 (MQ=255)                                                                                                 tGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGATTAATCTCTTTAACAAGCACGCCCGAAAACTAATGCCCGCCTGATCATCATTGAACCGCGCGATACCGAAACCCGGGCCCGGggcgga                                                            >  2:655553/1‑138 (MQ=255)                                                                                                  gCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATTTTGAACAAGCACGCCCGAAATCTAATGCCCGCATGATCATCATTGATCCCCGCTATACCCACACCGGTGCCGGGGGCGGAGATGAGTGGaac                                                >  1:376246/1‑149 (MQ=255)                                                                                                    tGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCCCGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATccc                                              >  1:853681/1‑151 (MQ=255)                                                                                                       ttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTa                                            <  2:299761/150‑1 (MQ=255)                                                                                                                     ggCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAAc                               <  1:907642/149‑1 (MQ=255)                                                                                                                          aaCGCGAATGAGTGGCGGTGGGGGGAGTTTCTATCTTTAAAAAGCACGCCAGAAATCTAATGCCCGCATGATCATCATAGATCCGCGCTATACCAACACCGGTGCCGGGCGCGAAGAAGAGTGGATCCCTATTCGTCCGGGGACAGATGc                         >  2:396909/1‑150 (MQ=255)                                                                                                                           aCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCg                       <  1:535500/151‑1 (MQ=255)                                                                                                                            cgcgAATGAGTGGCGGTGGGGGGACTTTCTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGGACAGATGCCGc                      >  1:304135/1‑151 (MQ=255)                                                                                                                                aaTGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTg                  <  1:931034/151‑1 (MQ=255)                                                                                                                                     gTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCCGAAATCTAATGCCCGCATGCTCATCATCGATCCCCGCTATACCGACACCCGCGCCCGGGGCGCAGAAGAGTGGGTCCCCATTCGTCCGGGGACAGATGCCGCACTTGtt               >  2:84005/1‑149 (MQ=255)                                                                                                                                                 tGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt  >  2:937576/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                      ACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941569‑941862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑