Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 663,746 C→T S73N (AGC→AAC)  dacA ← D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913663,7460CT98.8% 300.8 / ‑3.6 82S73N (AGC→AAC) dacAD‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (41/40);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

TGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGA  >  NC_000913/663603‑663893
                                                                                                                                               |                                                                                                                                                   
tGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAg                                                                                                                                               <  1:858126/150‑1 (MQ=255)
tGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAg                                                                                                                                               <  2:96317/150‑1 (MQ=255)
 gAGGAACATCCGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTAATCATTTTGGTTAGGTTGGCAgg                                                                                                                                              >  2:536933/1‑150 (MQ=255)
 gAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAgg                                                                                                                                              <  1:595357/150‑1 (MQ=255)
 gAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAgg                                                                                                                                              <  1:507030/150‑1 (MQ=255)
 gAGGAACATCAGCGAAAAACCTTTAAACACCGGATTACCGGTGGCCCATGCGTCGTGGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGCAAGGTTAGCAg                                                                                                                                               >  2:567137/1‑149 (MQ=255)
  aGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGAt                                                                                                                                            >  1:625634/1‑151 (MQ=255)
    gAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATcg                                                                                                                                          <  1:44281/151‑1 (MQ=255)
        aTCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCgcggc                                                                                                                                      <  2:524195/151‑1 (MQ=255)
              gaagaaCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACAt                                                                                                                                >  2:628589/1‑151 (MQ=255)
               aagaaCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATc                                                                                                                               >  2:294594/1‑151 (MQ=255)
               aagaaCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATc                                                                                                                               >  2:663648/1‑151 (MQ=255)
               aagaaCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATc                                                                                                                               <  2:898393/151‑1 (MQ=255)
                agaaCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACa                                                                                                                                 >  2:646516/1‑148 (MQ=255)
                   aCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCg                                                                                                                           >  1:903918/1‑151 (MQ=255)
                    ccTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGGCGGTAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCg                                                                                                                           <  2:80778/150‑1 (MQ=255)
                       ttAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCt                                                                                                                       >  2:713511/1‑151 (MQ=255)
                        tAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCt                                                                                                                       <  1:826480/150‑1 (MQ=255)
                         aaaCACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGt                                                                                                                     <  2:333182/151‑1 (MQ=255)
                           acacCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGGTc                                                                                                                   <  2:568428/151‑1 (MQ=255)
                           acacCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGACATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTc                                                                                                                   >  2:641132/1‑151 (MQ=255)
                              ccGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGc                                                                                                                >  2:438190/1‑151 (MQ=255)
                              ccGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGc                                                                                                                >  1:671272/1‑151 (MQ=255)
                                   ttACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGc                                                                                                            >  1:110406/1‑150 (MQ=255)
                                    tACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCAc                                                                                                          >  1:592789/1‑151 (MQ=255)
                                       cGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACttt                                                                                                       >  2:12069/1‑151 (MQ=255)
                                       cGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACttt                                                                                                       <  2:263037/151‑1 (MQ=255)
                                        ggTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTg                                                                                                      <  2:729124/151‑1 (MQ=255)
                                          tGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGcc                                                                                                    >  2:114856/1‑151 (MQ=255)
                                           ggCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGcc                                                                                                    <  2:787681/150‑1 (MQ=255)
                                            gCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGTCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCgg                                                                                                  <  1:267570/151‑1 (MQ=255)
                                                aTGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGGGAGCACTTTGCCGGAGtt                                                                                              <  1:282600/151‑1 (MQ=255)
                                                    gtcgtTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTtata                                                                                           <  1:52609/150‑1 (MQ=255)
                                                       gtTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGGTCTGTTCGGCGAGCACTTTGCCGGAGTTATAgg                                                                                         <  2:189168/149‑2 (MQ=255)
                                                        ttGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCa                                                                                       >  1:200862/1‑150 (MQ=255)
                                                             gATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACCTCTGCGTTCTGTTCCGCGAGCACTTTGCCGGAGTTATAATCAATCAgg                                                                                 >  2:697960/1‑151 (MQ=255)
                                                              aTAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTTATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAgg                                                                                 >  1:734119/1‑150 (MQ=255)
                                                              aTAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAgg                                                                                 >  2:555730/1‑150 (MQ=255)
                                                                aGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATg                                                                              >  2:743748/1‑151 (MQ=255)
                                                                   gACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGt                                                                             >  2:312381/1‑149 (MQ=255)
                                                                   gACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAg                                                                           <  1:696551/151‑1 (MQ=255)
                                                                    aCTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAgg                                                                          >  1:394858/1‑151 (MQ=255)
                                                                      tAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCCTCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGCTGTAGGAc                                                                        >  1:223439/1‑151 (MQ=255)
                                                                        aaTCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTc                                                                      >  2:228934/1‑151 (MQ=255)
                                                                        aaTCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTc                                                                      >  2:64897/1‑151 (MQ=255)
                                                                         aTCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTcc                                                                     >  2:605765/1‑151 (MQ=255)
                                                                         aTCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTcc                                                                     <  1:65558/151‑1 (MQ=255)
                                                                          tCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCg                                                                    >  2:858090/1‑151 (MQ=255)
                                                                           cAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCg                                                                    <  1:763370/150‑1 (MQ=255)
                                                                               ttCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATc                                                                <  1:622365/150‑1 (MQ=255)
                                                                               ttCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCg                                                               >  2:247285/1‑151 (MQ=255)
                                                                                   ttAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGAt                                                             <  2:184966/149‑1 (MQ=255)
                                                                                      aaTTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCg                                                        >  1:921689/1‑151 (MQ=255)
                                                                                      aaTTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCg                                                        <  1:244117/151‑1 (MQ=255)
                                                                                      aaTTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCg                                                        >  2:530543/1‑151 (MQ=255)
                                                                                          tACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTa                                                     <  2:142494/150‑1 (MQ=255)
                                                                                           aCCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTac                                                    >  2:289565/1‑150 (MQ=255)
                                                                                           aCCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTac                                                    >  1:948073/1‑150 (MQ=255)
                                                                                                cTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCgg                                              >  1:818726/1‑151 (MQ=255)
                                                                                                  ttCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCggg                                             >  2:860150/1‑150 (MQ=255)
                                                                                                  ttCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCggg                                             <  1:172178/150‑1 (MQ=255)
                                                                                                  ttCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCggg                                             >  1:436469/1‑150 (MQ=255)
                                                                                                        gCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAg                                      <  2:921442/151‑1 (MQ=255)
                                                                                                            ggCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGttt                                   <  2:698555/150‑1 (MQ=255)
                                                                                                             gCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTg                                 <  2:424927/151‑1 (MQ=255)
                                                                                                             gCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTg                                 <  2:866012/151‑1 (MQ=255)
                                                                                                                gATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGata                              <  2:145545/151‑1 (MQ=255)
                                                                                                                     cGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAg                         <  1:506485/151‑1 (MQ=255)
                                                                                                                        aaCTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTc                      <  1:310090/151‑1 (MQ=255)
                                                                                                                         aCTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCa                     <  1:655494/151‑1 (MQ=255)
                                                                                                                             gTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCgg                 <  1:355530/151‑1 (MQ=255)
                                                                                                                               catcatTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGc                >  2:370953/1‑150 (MQ=255)
                                                                                                                               catcatTTTGGTCAGGTTGCCAGGATCGCGGCGGACATCTCCGTTCTGTTCGACGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGCTCATAGTTTTGATATTTAGGTCATCg                  >  2:347665/1‑148 (MQ=255)
                                                                                                                                  catTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGGCATCGGCAtg             <  2:675193/150‑1 (MQ=255)
                                                                                                                                   atTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtgtg           <  2:981504/151‑1 (MQ=255)
                                                                                                                                        ggTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGCGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgcag        >  2:55453/1‑149 (MQ=255)
                                                                                                                                        ggTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgcagc       <  1:438304/150‑1 (MQ=255)
                                                                                                                                         gTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGcagcag     <  1:260205/151‑1 (MQ=255)
                                                                                                                                         gTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGGgcag        >  2:607018/1‑148 (MQ=255)
                                                                                                                                          tCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGACAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGCTTTGATATTCAGGTCATCGGCATgcgccgaag     >  2:725849/1‑145 (MQ=255)
                                                                                                                                           cAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCagag   <  2:671104/151‑1 (MQ=255)
                                                                                                                                            aGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAgaga  <  1:24592/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGA  >  NC_000913/663603‑663893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: