Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913589,9340TG42.9% 50.7 / 10.6 36T341P (ACC→CCC) nfrAbacteriophage N4 receptor, outer membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (1/19);  new base G (14/1);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 9.27e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAA  >  NC_000913/589784‑590078
                                                                                                                                                      |                                                                                                                                                  
gcagcaGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGt                                                                                                                                                    <  2:767645/151‑1 (MQ=255)
gcagcaGTTGCCAGGTTAGGTGGGCCGGGCGGGTAAGATGTGCCGGTTCTTGCTGACACAGCGAGCGTGCCGGTCGCAGAGTTTCAGCCTTGTTACGGGTCGCCACGCTGACAGGATCACGCGCCTCAAGCATTCCAGTGGCGGGGAGGGt                                                                                                                                                    <  2:524726/151‑1 (MQ=255)
gCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGt                                                                                                                                                    <  2:902586/151‑1 (MQ=255)
    cAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCg                                                                                                                                                <  2:866863/151‑1 (MQ=255)
     aGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCCTAACGCTCCTCCAGCATTTCCTTGGCGGGGGGGGCgggg                                                                                                                                                >  1:33863/1‑148 (MQ=255)
     aGTTGCCAGGTTAGTTGATCCAGGCGGGGAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGAAGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGgggggggggggg                                                                                                                                                >  2:623568/1‑148 (MQ=255)
       ttGCCAGGTTAGTTGATCCAGGCGGGGAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGggggggggggggggg                                                                                                                                             >  2:109531/1‑146 (MQ=255)
           cAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGtt                                                                                                                                         <  2:94621/151‑1 (MQ=255)
            aGGTTAGTTGATCCAGGCGGGGAAGATTTGCCGGTTCTTGCTGGTAAAGCAATTGTGCCAGACGCAGAGCTTCTGCCTTGTTACGGGTGGGCCCGCTGACAGCATAACGCTCCCCAAGCCTTTCTTTGGGGGGGGGGGGGGCgtggcggt                                                                                                                                         >  2:229046/1‑143 (MQ=255)
              gTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGttttt                                                                                                                                      <  1:545608/151‑1 (MQ=255)
              gTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGttttt                                                                                                                                      <  1:123269/151‑1 (MQ=255)
                    tGATCCAGGCGGGGAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGggggggggggggggggggtttt                                                                                                                                      >  2:212706/1‑133 (MQ=255)
                       tCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGcg                                                                                                                              <  2:475580/150‑1 (MQ=255)
                                gTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGGTTTTTCGCTGCG‑GCGTACTGAc                                                                                                                   >  1:648888/1‑151 (MQ=255)
                                     aTTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACcttct                                                                                                              <  1:821469/151‑1 (MQ=255)
                                         gCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTa                                                                                                          <  2:193727/151‑1 (MQ=255)
                                             gTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCGGGTTTTTCGCTGCG‑TCGTACCGTACTtttttt                                                                                                           >  2:249463/1‑146 (MQ=255)
                                             gTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGCTTTTGCGCTGGG‑TTGTAGCGACCTTCtttttata                                                                                                       >  1:776391/1‑146 (MQ=255)
                                              ttCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGTGGGGAGCAGATTTTTCGCTGCGTTCGTCTTGACCTTTCTTTacatc                                                                                                      >  2:56413/1‑147 (MQ=255)
                                              ttCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGGCGGTTTTTTGCCTCG‑GCGTACTGACCTTCttttttca                                                                                                       >  2:557258/1‑145 (MQ=255)
                                               tCTTGCTGATACAGCAATCGGGGCAGACGCAGTGCTTCAGCCCTGTTCCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATGTCATTGGCGGGGAGGGGGGCGAGCAGTTTCGGCGCTGCT‑TCTTACTGACCTTCTTTGCACAGc                                                                                                     <  2:540437/150‑1 (MQ=255)
                                                         aCAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCg                                                                                          <  2:361704/150‑1 (MQ=255)
                                                          cAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTc                                                                                        <  1:284775/151‑1 (MQ=255)
                                                           aGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGAAGTTTTTTCGGCCCG‑TCGCAATCAACTTCTTTTTACAAC‑ACCCGGAgcggcg                                                                                       >  1:925576/1‑151 (MQ=255)
                                                               aTCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGGGGGGAGGAGGTTTTTTGCTGCG‑GCGTACTGAACCTCTTTTTACAGCAACCGGT‑GCGGCGCGcc                                                                                   >  1:138487/1‑151 (MQ=255)
                                                                   tGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGCAGGTTTTGCGCCGCG‑TCGTACTGACCCTCTTTTTACAAC‑ACCGGGAGCGTCGCGcc                                                                                   >  1:301184/1‑147 (MQ=255)
                                                                      cAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGCGAGGGTGGCGACCAGTTTTTGCGCTACG‑TCGTACTGACCTTCTTTTAACAAC‑ACCAGTAGCGTCTCCCCAacaaca                                                                            <  2:174725/151‑1 (MQ=255)
                                                                          cGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACt                                                                        <  1:906233/151‑1 (MQ=255)
                                                                                 cTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTGTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCgg                                                                   <  2:902536/149‑1 (MQ=255)
                                                                                  ttCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCCGGTTTTTCGCCGGG‑TCGTACTGAACTTCTTTTTAAAGC‑ACCGGGAGGGGCGCGGCCACAACAAACTGGCgg                                                                   >  2:362275/1‑148 (MQ=255)
                                                                                  ttCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGCAGTTTTTTCGCTGCG‑TCGTACTGACCTTCTTTTTACAGC‑ACCGGGAGCGGCGCGCCCACAACATACTGGCggg                                                                  >  1:518008/1‑148 (MQ=255)
                                                                                        cTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTTGCTGCG‑TCGGACTGACCCTTTTTTTAAAGC‑AACGGGGGGGGCGCGCCCACAACAAACTGGCg                                                                    >  1:815547/1‑141 (MQ=255)
                                                                                        cTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGGGGGGAGCAGTTTTTTGGCTCCG‑GCGTCCTTACCCTCTTTTTAAAGC‑ACCGGGGGCGTTGCGCCCACCACATAATGGCGGgtgt                                                               >  2:809177/1‑146 (MQ=255)
                                                                                         ttGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCCGATTTTTCGCTGCC‑TCCCTCTTACCTTCTTTTAACAGC‑CCCGGTAGGCTCGCGCCACAAACAACCTTGCgggttg                                                               >  2:51507/1‑141 (MQ=255)
                                                                                              aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTg                                                     <  2:33857/150‑1 (MQ=255)
                                                                                                gggTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGGAGGTTTTGCGCTGCC‑TCGTGTTTACCTTCTTTTTAAAGC‑ACCCGGAGCGGCGCGCCAACAACATACTGGCgggggggtgggga                                                        >  1:230333/1‑134 (MQ=255)
                                                                                                  gTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCg                                                <  2:464648/151‑1 (MQ=255)
                                                                                                                      aCGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGa                            <  1:933251/151‑1 (MQ=255)
                                                                                                                               aaGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTa                   <  2:31908/151‑1 (MQ=255)
                                                                                                                                          tGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTAtt         <  2:862084/150‑1 (MQ=255)
                                                                                                                                               gggAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATa   <  1:16385/151‑1 (MQ=255)
                                                                                                                                                ggAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATaa  <  2:299750/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCG‑TCGTACTGACCTTCTTTTAACAGC‑ACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAA  >  NC_000913/589784‑590078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑