Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 646,777 | 0 | G | T | 40.4% | 68.5 / 27.8 | 52 | Q245K (CAA→AAA) | citG | 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase |
Reads supporting (aligned to +/- strand): ref base G (9/22); new base T (21/0); total (30/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.80e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCA > NC_000913/646627‑646926 | ataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGACCTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTg < 1:804410/151‑1 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTgcgc < 1:467184/151‑1 (MQ=255) aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGGCTGAGATTTCGTTCGATACACTCCCTTTCGAACTGCCGGAGATAATCGAGATCGGCGTGGG‑TTCGAA‑TGCACCCTTTTTGCAATAATGTTTGCGCCt < 1:653627/150‑1 (MQ=255) aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTc < 1:608234/151‑1 (MQ=255) aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGcccc > 1:893906/1‑149 (MQ=255) gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTa < 2:298213/151‑1 (MQ=255) tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGCCCCccgc > 1:499719/1‑145 (MQ=255) tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCCGTAg > 1:224902/1‑150 (MQ=255) tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAAAGTTTTCGCCTCGCGCTGTAGc > 1:587552/1‑151 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAg < 1:425358/149‑1 (MQ=255) ccAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTTGGA‑AGCCCCCCTTTTTCCATAATTTTTTCGcccccccccgttgcc > 1:354248/1‑133 (MQ=255) cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTTGGA‑AGCCCCCCCTTTCCCATAAACTTTTGGCCgcgcgcgccag > 2:679754/1‑138 (MQ=255) aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGCGATCGGCGGGGG‑TTGGGA‑GGGGCCCTCTTTTTAATAATATGTTTTCCCCGGCCCGTAGCCAGCGCAGGcc > 2:629092/1‑150 (MQ=255) aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGGA‑TGCCCCCCTTTTTCAATAAAGTTTTCTCCCCCCGCCGGAGccccacccggcac > 2:722498/1‑140 (MQ=255) aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAAGTGCCCCCTTTTT‑TAATAAAGTTTTCCCCTCCCGCCGTAGGCAGCCCAGGccc > 2:249506/1‑151 (MQ=255) gATCAGTAGGTCATCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGGCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcccacc < 2:337022/151‑1 (MQ=255) ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCAcc < 1:817981/151‑1 (MQ=255) ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGACCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCAcc < 1:495567/151‑1 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGATTGGAG‑GGCCCCCTTTTT‑CAATAATTTTTTCGCCTCCCCCTGTTGGCCCCGCCGGcccccccccccccccc > 1:740660/1‑140 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgc < 2:847618/150‑1 (MQ=255) cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGa < 1:877821/151‑1 (MQ=255) ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCTTTTTGTAATAATATTTTTGCCCCCCGCCGTTGACAGCGCAGGCCCCCCCCTCCCCCCCCCGATGc > 2:69061/1‑149 (MQ=255) gccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCACCCCCCGATGCAAc > 1:342902/1‑150 (MQ=255) cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAAAATTTTCGCCTCGCGCTGGAGCCCGCGCAGGCCCCCCTCGCCACCCCCCGCTGCAACGCTggga > 1:237258/1‑148 (MQ=255) agaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCCATAAATTTTTCGCCCCGCGCCGTAGCCCGCGCAGGGCCCCCCCCCCCCCCCGCGATGCAACGGTgg > 1:884702/1‑145 (MQ=255) aTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGATGCAACGTTGGGATCGcc > 1:945304/1‑150 (MQ=255) tttCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGAGG‑GTCGCA‑TTCCCCCTCTTTTCCATAATGTTTTCGCCTCGCGCCGTAAACCGCGCCGGACCCCCCCGCCCCCGCACGCAGCAACGCTgggttcccc > 1:224265/1‑142 (MQ=255) ttcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTTGAA‑TGCCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCGCCACCCCCCGATGCAACGTTGGGATCGcc > 1:610625/1‑148 (MQ=255) ttcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCTAA‑TTCCCCCCTTTTTCAATAAATTTTTTGCCCCCCGCCGTAGCCCGCCCCCGCGCCCCCCCCCCCCCCGCCATGCAACGTTGGGGTCGCCggt > 2:8420/1‑149 (MQ=255) aTACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCCCA‑TGACCCCCTTTTTCAATAATGTTTTTGCCCCCCGCCGTAGCCAGCGCCGGGCCCCCCCCCCACCCCGCCGGGCAACGTTGGGATATCCGCTGATAGccc > 2:916846/1‑150 (MQ=255) acacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 1:540766/150‑1 (MQ=255) tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGGA‑ATCCCCCCTTTTCTAATCAAATTTTCGCCTCGCGCCGTTGCCAGCGCCGGCCCCCCCCCCCACCCCGCGGGGCCACGCTGGGATCGccccggctcgcccccc > 2:429336/1‑134 (MQ=255) cccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAAAGTTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCACCCCGCGGGGCCACGGTGGTATCGCCGTTGATCGcc > 1:255148/1‑143 (MQ=255) gagaTAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc < 2:420263/151‑1 (MQ=255) agaTAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc < 2:128868/150‑1 (MQ=255) aaTCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATg < 1:325817/150‑1 (MQ=255) agaTCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt < 2:71945/151‑1 (MQ=255) gaTCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTTCCATAATGTTTGCGCCCCGCGCTGTAACCAGCGCCGGCCCCCCTCGCCCCCGCGCGATGCAACGTTGGGATCGCCGTTGATCGCCATCCGTAGGGGCAAGGGATCGAGGAATggccggt > 2:584099/1‑144 (MQ=255) cggcggGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAgg < 1:99818/151‑1 (MQ=255) gcggGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGAt > 1:67939/1‑151 (MQ=255) cggGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATc > 1:160711/1‑151 (MQ=255) ggggg‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCt > 1:340605/1‑151 (MQ=255) g‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAcc < 1:426686/151‑1 (MQ=255) cGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCt < 1:799014/151‑1 (MQ=255) gAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTg > 2:179529/1‑151 (MQ=255) gAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTg < 1:485308/151‑1 (MQ=255) aa‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGa < 1:344571/151‑1 (MQ=255) ccccTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATcc > 1:716453/1‑149 (MQ=255) ccTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagca < 1:96841/151‑1 (MQ=255) ttttGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagcag < 1:304811/149‑1 (MQ=255) gCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCCCCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCatag < 2:471617/147‑1 (MQ=255) ataatGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCACTGATCCAGCAGAGTGa > 2:336556/1‑147 (MQ=255) taatGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTa > 2:526515/1‑150 (MQ=255) aatGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAAt > 2:439190/1‑151 (MQ=255) gcgcCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCa < 1:419812/150‑1 (MQ=255) | ATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCA > NC_000913/646627‑646926 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |