Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913646,7770GT40.4% 68.5 / 27.8 52Q245K (CAA→AAA) citG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
Reads supporting (aligned to +/- strand):  ref base G (9/22);  new base T (21/0);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 7.80e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCA  >  NC_000913/646627‑646926
                                                                                                                                                        |                                                                                                                                                     
ataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGACCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTg                                                                                                                                                       <  1:804410/151‑1 (MQ=255)
   atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTgcgc                                                                                                                                                    <  1:467184/151‑1 (MQ=255)
      aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGGCTGAGATTTCGTTCGATACACTCCCTTTCGAACTGCCGGAGATAATCGAGATCGGCGTGGGTTCGAATGCACCCTTTTTGCAATAATGTTTGCGCCt                                                                                                                                                  <  1:653627/150‑1 (MQ=255)
      aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTc                                                                                                                                                 <  1:608234/151‑1 (MQ=255)
      aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGcccc                                                                                                                                                 >  1:893906/1‑149 (MQ=255)
              gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTa                                                                                                                                         <  2:298213/151‑1 (MQ=255)
                tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCccgc                                                                                                                                             >  1:499719/1‑145 (MQ=255)
                tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCCGTAg                                                                                                                                        >  1:224902/1‑150 (MQ=255)
                tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAAAGTTTTCGCCTCGCGCTGTAGc                                                                                                                                       >  1:587552/1‑151 (MQ=255)
                 aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAg                                                                                                                                        <  1:425358/149‑1 (MQ=255)
                      ccAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGAAGCCCCCCTTTTTCCATAATTTTTTCGcccccccccgttgcc                                                                                                                                      >  1:354248/1‑133 (MQ=255)
                       cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGAAGCCCCCCCTTTCCCATAAACTTTTGGCCgcgcgcgccag                                                                                                                                        >  2:679754/1‑138 (MQ=255)
                            aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGCGATCGGCGGGGGTTGGGAGGGGCCCTCTTTTTAATAATATGTTTTCCCCGGCCCGTAGCCAGCGCAGGcc                                                                                                                            >  2:629092/1‑150 (MQ=255)
                            aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTCAATAAAGTTTTCTCCCCCCGCCGGAGccccacccggcac                                                                                                                           >  2:722498/1‑140 (MQ=255)
                            aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAGTGCCCCCTTTTTTAATAAAGTTTTCCCCTCCCGCCGTAGGCAGCCCAGGccc                                                                                                                           >  2:249506/1‑151 (MQ=255)
                               gATCAGTAGGTCATCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGGCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcccacc                                                                                                                        <  2:337022/151‑1 (MQ=255)
                                       ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCAcc                                                                                                                <  1:817981/151‑1 (MQ=255)
                                       ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGACCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCAcc                                                                                                                <  1:495567/151‑1 (MQ=255)
                                          cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGATTGGAGGGCCCCCTTTTTCAATAATTTTTTCGCCTCCCCCTGTTGGCCCCGCCGGcccccccccccccccc                                                                                                              >  1:740660/1‑140 (MQ=255)
                                          cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgc                                                                                                              <  2:847618/150‑1 (MQ=255)
                                             cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGa                                                                                                          <  1:877821/151‑1 (MQ=255)
                                                  ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCTTTTTGTAATAATATTTTTGCCCCCCGCCGTTGACAGCGCAGGCCCCCCCCTCCCCCCCCCGATGc                                                                                                       >  2:69061/1‑149 (MQ=255)
                                                    gccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCACCCCCCGATGCAAc                                                                                                    >  1:342902/1‑150 (MQ=255)
                                                           cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAAAATTTTCGCCTCGCGCTGGAGCCCGCGCAGGCCCCCCTCGCCACCCCCCGCTGCAACGCTggga                                                                                             >  1:237258/1‑148 (MQ=255)
                                                              agaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCCATAAATTTTTCGCCCCGCGCCGTAGCCCGCGCAGGGCCCCCCCCCCCCCCCGCGATGCAACGGTgg                                                                                               >  1:884702/1‑145 (MQ=255)
                                                                aTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGATGCAACGTTGGGATCGcc                                                                                        >  1:945304/1‑150 (MQ=255)
                                                                 tttCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGAGGGTCGCATTCCCCCTCTTTTCCATAATGTTTTCGCCTCGCGCCGTAAACCGCGCCGGACCCCCCCGCCCCCGCACGCAGCAACGCTgggttcccc                                                                                        >  1:224265/1‑142 (MQ=255)
                                                                  ttcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCGCCACCCCCCGATGCAACGTTGGGATCGcc                                                                                        >  1:610625/1‑148 (MQ=255)
                                                                  ttcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCTAATTCCCCCCTTTTTCAATAAATTTTTTGCCCCCCGCCGTAGCCCGCCCCCGCGCCCCCCCCCCCCCCGCCATGCAACGTTGGGGTCGCCggt                                                                                     >  2:8420/1‑149 (MQ=255)
                                                                          aTACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCCCATGACCCCCTTTTTCAATAATGTTTTTGCCCCCCGCCGTAGCCAGCGCCGGGCCCCCCCCCCACCCCGCCGGGCAACGTTGGGATATCCGCTGATAGccc                                                                             >  2:916846/1‑150 (MQ=255)
                                                                            acacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt                                                                            <  1:540766/150‑1 (MQ=255)
                                                                                tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAATCCCCCCTTTTCTAATCAAATTTTCGCCTCGCGCCGTTGCCAGCGCCGGCCCCCCCCCCCACCCCGCGGGGCCACGCTGGGATCGccccggctcgcccccc                                                                          >  2:429336/1‑134 (MQ=255)
                                                                                 cccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAAAGTTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCACCCCGCGGGGCCACGGTGGTATCGCCGTTGATCGcc                                                                              >  1:255148/1‑143 (MQ=255)
                                                                                                 gagaTAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc                                                      <  2:420263/151‑1 (MQ=255)
                                                                                                  agaTAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc                                                      <  2:128868/150‑1 (MQ=255)
                                                                                                      aaTCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATg                                                  <  1:325817/150‑1 (MQ=255)
                                                                                                           agaTCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt                                            <  2:71945/151‑1 (MQ=255)
                                                                                                            gaTCGGCGGGGGTTCGAATGCCCCCTTTTTTCCATAATGTTTGCGCCCCGCGCTGTAACCAGCGCCGGCCCCCCTCGCCCCCGCGCGATGCAACGTTGGGATCGCCGTTGATCGCCATCCGTAGGGGCAAGGGATCGAGGAATggccggt                                            >  2:584099/1‑144 (MQ=255)
                                                                                                               cggcggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAgg                                        <  1:99818/151‑1 (MQ=255)
                                                                                                                 gcggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGAt                                      >  1:67939/1‑151 (MQ=255)
                                                                                                                  cggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATc                                     >  1:160711/1‑151 (MQ=255)
                                                                                                                   gggggTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCt                                    >  1:340605/1‑151 (MQ=255)
                                                                                                                       gTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAcc                                <  1:426686/151‑1 (MQ=255)
                                                                                                                           cGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCt                             <  1:799014/151‑1 (MQ=255)
                                                                                                                            gAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTg                            >  2:179529/1‑151 (MQ=255)
                                                                                                                            gAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTg                            <  1:485308/151‑1 (MQ=255)
                                                                                                                             aaTGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGa                           <  1:344571/151‑1 (MQ=255)
                                                                                                                                   ccccTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATcc                        >  1:716453/1‑149 (MQ=255)
                                                                                                                                     ccTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagca                    <  1:96841/151‑1 (MQ=255)
                                                                                                                                        ttttGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagcag                   <  1:304811/149‑1 (MQ=255)
                                                                                                                                            gCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCCCCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCatag                 <  2:471617/147‑1 (MQ=255)
                                                                                                                                               ataatGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCACTGATCCAGCAGAGTGa              >  2:336556/1‑147 (MQ=255)
                                                                                                                                                taatGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTa          >  2:526515/1‑150 (MQ=255)
                                                                                                                                                 aatGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAAt        >  2:439190/1‑151 (MQ=255)
                                                                                                                                                        gcgcCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCa  <  1:419812/150‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
ATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCA  >  NC_000913/646627‑646926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: